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ProteomicsDSSBU: novel cross-linker tested on bovine serum albumin and human hemoglobin
Protein cross-linking has assumed an irreplaceable role in structural proteomics. Recently, significant efforts have been made to develop novel MS-cleavable reagents. These cross-linkers enhance the reliability of cross-link identification. Presently, only water-insoluble MS-cleavable cross-linkers ...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2025-05-07 | PXD055284 | Pride
Dssbu Hemoglobin Xl-ms Ms-cleavable cross-linker Bsa 
ProteomicsDSSBU: An MS-Cleavable Analogue of the BS3 Cross-Linker
In this study, we introduce a new MS-cleavable cross-linker called disulfodisuccinimidyl dibutyric urea (DSSBU), which we developed in-house. DSSBU contains an N-hydroxysulfosuccinimide (sulfo-NHS) reactive group that primarily modifies lysine residues. It therefore serves as a water-soluble counter...
ORGANISM(S): Bos taurus (Bovine) 
2025-12-29 | PXD052450 | Pride
Bsa Protein cross-linking Dssbu cross-linker 
ProteomicsA Glycosidic-Bond-Based Mass-Spectrometry-Cleavable Cross-linker Enables In Vivo Cross-linking for Protein Complex Analysis
Chemical cross-linking mass spectrometry (CXMS) has emerged as a powerful technology to analyze protein complex structure and interaction. However, the spectral fragmentation behavior and spectral data retrieval of cross-linked peptides are more complex than single peptides. In this study, we design...
ORGANISM(S): Homo Sapiens Saccharomyces Cerevisiae 
2023-12-06 | PXD047554 |
ProteomicsCross-linking mass spectrometry analyses of three different kinetochore protein complexes (KMN, NDC80C, MIS12C) using an MS-cleavable cross-linker, BuUrBu (DSBU)
In order to develop a simplified cross-linking mass spectrometry protocol, we applied one-step size-exclusion chromatography (SEC) for peptide purification after proteolysis with Lys-C and trypsin. Three benchmark protein complexes (KMN, NDC80C, MIS12C) from human kinetochore were cross-linked with ...
ORGANISM(S): Homo sapiens (Human) 
2019-04-01 | PXD010070 | Pride
Dsbu Q-exactive Cross-linking ms Buurbu Merox Human Kinetochore Protein complex Ms-cleavable cross-linker 
ProteomicsHigher dimensional cross-linking with a tetra-reactive cross-linker
A tetrameric CID-cleavable cross-linker was synthesized and applied to BSA and isolated mitochondria from mouse hearts. A real-time instrument method was developed to dynamically target released peptides from cross-linked species, enabling their characterization through a series of ms3 scans.
ORGANISM(S): Bos taurus (Bovine) Mus musculus (Mouse) 
2023-11-27 | PXD032222 | Pride
Mitochondria Cross-linking 
ProteomicsDevelopment of an sulfoxide-based, CuAAC-enrichable cross-linker
Cross-linking of BSA with a novel cross-linker. Modification of the cross-linker containing peptides with CuAAC-chemistry to attach a cleavable biotin-derivative. Enrichment with streptavidin-beads.
ORGANISM(S): Bos taurus (Bovine) 
2019-10-14 | PXD015080 | Pride
Cuaac Xl-ms Sulfoxide 
ProteomicsCleavable Photo-Crosslinker Enables sFFP-Driven Strategy for Mapping HSP90β Oligomer Structure
Crosslinking technology is a pivotal tool for resolving dynamic protein interaction networks. While traditional chemical crosslinkers face limitations in sensitivity, reaction kinetics, and in situ activation, cleavable and photo-crosslinkers have emerged as alternatives. However, their complex spec...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2026-04-09 | PXD064219 | Pride
Photo-cross-link Hsp90 Randomforest Cleavable cross-linker 
Proteomics2) Twisting Urea- to Imide-Based Mass Spectrometry-Cleavable Cross-Linkers to Embed Affinity Tags
Disuccinimidyl dibutyric urea (DSBU) is a mass spectrometry (MS)-cleavable cross-linker that has been applied to multiple applications in structural biology, ranging from isolated protein complexes to comprehensive system-wide interactomics. DSBU facilitates a rapid and reliable identification of cr...
ORGANISM(S): Homo sapiens (Human) 
2024-09-03 | PXD050960 | Pride
Protein-protein interactions  structural proteomics Dsbu Cross-linking mass spectrometry  xlms Idp Intrinsically disordered protein 
ProteomicsCross-linking mass spectrometry analysis of human ADAR1 deaminase domain WT using an MS-cleavable cross-linker, DSBU
Cross-linking mass spectrometry analysis of human ADAR1 deaminase domain WT protein (hADAR1d WT) was carried out using an MS-cleavable cross-linker, DSBU, to support the homology model structure of hADAR1d WT.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-08-21 | MSV000085996 | MassIVE
ProteomicsCharacterizing the dynamic oligomerization of Hsp90β using photo-cross-linking
Cross-linking mass spectrometry (XL-MS) is a powerful tool for probing protein structures. While conventional chemical cross-linkers react with specific residues with defined chemistry, photo-cross-linkers, despite their superior reactivity, have been hindered by incomplete mechanistic understanding...
ORGANISM(S): Homo sapiens (Human) 
2026-04-09 | PXD072009 | Pride
Hsp90 Cleavable cross-linkers Diazirine Photo-cross-linking 
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