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ProteomicsMass spectrometry proteomics detecting interaction proteins of Kindlin-2
We aim to find out the Kindlin-2 interaction proteins. We transfected plasmids Flag or Flag-Kindlin-2 to HKC cell lines and then used Flag-M2 beads to do the coimmunoprecipitation experiments and followed by NuPAGE Tris-Acetate Mini Gels detection and stained by Coomassie brilliant blue. Compared th...
ORGANISM(S): Homo sapiens (Human) 
2019-12-16 | PXD016008 | Pride
Human renal tubular epithelial cell(hkc) 
ProteomicsPEPPI-MS- and GELFrEE-fractionated E. coli MG1655 whole cell lysate
Supplemental Files accompanying manuscript “PEPPI-MS: polyacrylamide gel-based prefractionation for analysis of intact proteoforms and protein complexes by mass spectrometry”.
ORGANISM(S): Escherichia Coli Str. K-12 Substr. Mg1655 (ncbitaxon:511145) 
2020-04-08 | MSV000085258 | MassIVE
ProteomicsComplementarity of different SDS-PAGE gel staining methods for the identification of short open reading frame-encoded peptides
Short open reading frame-encoded peptides (SEP) have been identified across all domains of life and are predicted to be involved in many biochemical processes, however, for the vast majority of SEP their biological function is still unknown. Special methodologies have to be used for the mass spectro...
ORGANISM(S): Methanosarcina mazei Go1 
2023-07-06 | PXD018269 | Pride
Gelc-ms  microproteins  peptidomics  sorf  smorf 
UnknownQuantification of AAV particle titers by infrared fluorescence scanning of coomassie-stained sodium dodecyl sulfate-polyacrylamide gels.
Not available
S-EPMC4015068 | biostudies-literature
ProteomicsMass spectrometry analysis of in vitro PKCe phosphorylated ADSL
Bacterially purified His-ADSL proteins on Ni-NTA agarose beads were incubated with or without active PKCε in the presence of ATP for an in vitro kinase assay. After centrifugation, the agarose beads were collected and mixed with 30 μL of SDS-PAGE sample loading buffer. The samples were then separate...
ORGANISM(S): Homo sapiens (Human) 
2026-02-16 | PXD074509 | Pride
In vitro kinase assay  lc-ms/ms  phosphorylation 
ProteomicsIdentification of phosphorylation sites of BAG3
HEK293 cells were cultured in two 10-cm dishes and transfected with BAG3-FLAG as well as Myc vector, CaMKIIβ(D-S)-Myc, and CaMKIIδ(D-S)-MYC, respectively. After the cells were transfected for 24 h, they were treated with the indicated regent. The cells were lysed with NP-40 alternative cell lysis bu...
ORGANISM(S): Homo sapiens (Human) 
2024-08-20 | PXD054722 | Pride
Phosphorylation sites Bag3 Human 
ProteomicsIdentification of the ubiquitination sites of EV71-VP1
EV71-VP1-Flag plasmid was transfected into 293T cells for 36 hours and then treated with MG132 for 12 hours. After drug treatment, cells were collected, cell lysates were incubated with FLAG-Beads overnight and competed with FLAG peptides for 4 hours the next day. Proteins were separated by SDS-PAGE...
ORGANISM(S): Human Enterovirus 71 (strain 7423/ms/87) 
2025-01-07 | PXD059532 |
ProteomicsIdentification of a novel Glis3-348aa protein in MIN6 cells by LC-MS
To identify the novel protein encoded by circGlis3, total protein lysates were extracted from MIN6 cells transfected with circGlis3 plasmid or empty vector and separated by SDS-PAGE, followed by Coomassie brilliant blue staining. The differential gel bands near 35 kDa were excised and subjected to m...
ORGANISM(S): Mus musculus (Mouse) 
2023-11-02 | PXD037447 | Pride
Min6 cells Lc-ms Translation Circglis3 
ProteomicsAurora A phosphorylates PKM2 at T45.
Flag-PKM2 was expressed in H1299 cells. Then the H1299 cells were treated with DMSO or 100nM MLN8237. Then, cell lysate was incubated with anti-Flag M2-agarose. Elutes were separated with 10% SDS-PAGE. The gel was stained with Coomassie Brilliant Blue. Visualized bands were excised and subjected to ...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD036575 | Pride
Aurora a  pkm2 
ProteomicsImmunoprecipitation of STING/ADSL and mass spectrometry analysis
BT-549 cells with or without expression of Flag-STING were treated with or without hypoxia for 6 h. An immunoprecipitation assay was performed using anti-Flag antibody, and immunoprecipitates of Flag-STING were eluted with Flag peptide, separated using SDS-PAGE and stained with Coomassie Brillian...
ORGANISM(S): Homo sapiens (Human) 
2025-02-15 | PXD059420 | Pride
Mass spectrometry Protein-protein interaction Phopshorylation 
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