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ProteomicsMouse cerebellum (phospho)proteome LC-MSMS
Cerebella from wild-type and cGKI knockout mice, on 129/Sv background, were subjected to protein digestion using trypsin o/n and chemically labeled with dimethyl labeling. After labeling the samples were mixed and subjected to both strong cation exchange and phosphopeptide enrichment. The SCX frac...
ORGANISM(S): Mus musculus (Mouse) 
2014-06-17 | PXD000502 | Pride
Cerebellum Lc-msms Phosphoproteome Cgki knock out Proteome 
ProteomicsCharting the interactome of PDE3A in human cells using an IBMX based chemical proteomics approach
We attempt to study the interactome of PDEs by synthesizing a broad spectrum PDE-capturing resin based on the non-selective PDE inhibitor 3-isobutyl-1-methylxanthine (IBMX). Chemical proteomics characterization of this resin in HeLa cell lysates led to the capture of several different PDEs. Combinin...
ORGANISM(S): Homo sapiens (Human) 
2016-05-25 | PXD001781 | Pride
Ibmx Mass spectrometry Phosphodiesterase Papaverine Cilostamide Chemical proteomics 
ProteomicsInteraction of cGMP-dependent protein kinase and HAP1
Here, we use cAMP-based chemical proteomics approach, employing competitive concentrations of free cyclic nucleotides to isolate PKA and PKG and their secondary interactors.
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2015-02-09 | PXD001434 | Pride
Pkg Mass spectrometry Cgmp Gkap Chemical proteomics Hap1 
OtherHuntingtin associated protein (HAP1) is a cGMP-dependent kinase anchoring protein (GKAP), specific for the cGMP-dependent protein kinase Iβ isoform.
Data from ProteomeXchange, PXD ID: PXD001434. Published as part of J Biol Chem. 2015 Feb 4. pii . From the Abstract: {{i}} ... Here, we extend on this cAMP-based chemical proteomics approach, employing competitive concentrations of free cyclic nucleotides to isolate each kinase and its secondary in...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32320007631 | GPMDB
Resource Reanalysis 
ProteomicsMitochondrial CaMKII phosphoproteome.
Mice overexpressing CaMKII in myocardial mitochondria have dilated cardiomyopathy that appears to be exclusively driven by metabolic defects that lead to reduced ATP production. Phosphoproteomics study of mitochondrial proteins supports the metabolomics data.
ORGANISM(S): Mus musculus (Mouse) 
2020-07-31 | PXD004631 | Pride
Heart Tiimac Phosphoproteome Mitochondria Lc-ms/ms 
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