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MetabolomicsAftpo3 and Afdur3, the Aspergillus fumigatus Plasma Membrane Transporters of Polyamines, Regulate Polyamine Homeostasis and Itraconazole Susceptibility

Aspergillus fumigatus is a notorious opportunistic pathogen that causes Invasive Aspergillosis (IA) infections with high mortality in immunosuppressed individuals. Long-term antifungal drug azole abuse in clinical treatment and agriculture renders it ineffectual or drug resistant. Drug resistance...

2020-12-21 | MTBLS1739 | MetaboLights
ProteomicsPlasma and crude membrane fraction from mouse liver and kidney
The purpose of the present study was to validate the enrichment of transporter proteins in plasma membrane fraction prepared from mouse liver and kidney with Membrane Protein Extraction Kit by means of beads homogenize for efficient subcellular fractionation. The crude and plasma membrane fractions ...
ORGANISM(S): Mus Musculus (mouse) 
2020-09-26 | PXD018161 | JPOST Repository
MetabolomicsThe Impacts of Sortase A and the 4'-Phosphopantetheinyl Transferase Homolog Sfp on Streptococcus mutans Extracellular Membrane Vesicle Biogenesis

Extracellular membrane vesicles (EMVs) are produced by many Gram-positive organisms, but information regarding vesiculogenesis is incomplete. We used single gene deletions to evaluate the impacts on Streptococcus mutans EMV biogenesis of Sortase A (SrtA), which affects S. mutans...

2021-10-06 | MTBLS1812 | MetaboLights
ProteomicsRNAi-mediated downregulation of poplar plasma membrane intrinsic proteins (PIPs) changes plasma membrane proteome composition and affects leaf physiology
Plasma membrane intrinsic proteins (PIPs) are one subfamily of aquaporins that mediate the transmembrane transport of water. To reveal their function in poplar, we generated transgenic poplar plants in which the translation of PIP genes was downregulated by RNA interference investigated these plants...
ORGANISM(S): Populus canescens (Grey poplar) (Populus tremula x Populus alba) 
2015-08-10 | PXD002544 | Pride
Plasma membrane intrinsic protein  populus × canescens  plasma membrane proteomics  photosynthesis  stomatal movement Aquaporin  pip 
ProteomicsA Peptidisc-based Survey of the Plasma Membrane Proteome of a Mammalian Cell
The low abundance and hydrophobicity of plasma membrane proteome relative to soluble proteins makes it difficult to characterize. Here, we apply the peptidisc membrane mimetic to purify the cell membrane proteome. We used HeLa cells as a reference to establish the method, and compared the plasma mem...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD039990 | Pride
Plasma membrane proteome Peptidisc 
ProteomicsImproved plasma membrane proteome coverage with a label-free non-affinity-purified workflow
The proteins of the cellular plasma membrane perform important functions relating to homeostasis and intercellular communication. Due to its overall low cellular abundance, amphipathic character, and low membrane-to-cytoplasm ratio, the plasma membrane proteome has been challenging to isolate and ch...
ORGANISM(S): Homo sapiens (Human) 
2017-01-31 | PXD004801 | Pride
Label-free  proteomics  plasma membrane  go term  leukemia 
ProteomicsiTRAQ-based proteomic study investigate the effects of pre-partum dietary crude protein level on colostrum fat globule membrane proteins
Purpose: This study aimed to investigate the variation of MFGM proteins in colostrum of Hu sheep under different dietary crude protein levels. Experimental design: Collecting the colostrum from Hu sheep fed three different levels of dietary crude protein levels. Plasma membrane proteins were enriche...
ORGANISM(S): Ovis Aries 
2022-10-17 | PXD037465 |
ProteomicsA Peptidisc-based Survey of the Plasma Membrane Proteome of a Mammalian Cell
The low abundance and hydrophobicity of plasma membrane proteome relative to soluble proteins makes it difficult to characterize. Here, we apply the peptidisc membrane mimetic to purify the cell membrane proteome. We used HeLa cells as a reference to establish the method, and compared the plasma mem...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD041913 | Pride
Hela Peptidiscs Membrane proteins Hpsc Panc-1 cells Lc-ms/ms 
ProteomicsSTUDY OF THE PLASMA MEMBRANE PROTEOME DYNAMICS REVEALS NOVEL TARGETS OF THE NITROGEN REGULATION IN YEAST
Yeast cells, to be able to grow on a wide variety of nitrogen sources, regulate the set of nitrogen transporters present at their plasma membrane. Such regulation relies on both transcriptional and post-translational events. While large-scale microarray studies have identified most nitrogen-sensitiv...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2017-07-10 | PXD005273 | Pride
Yeast plasma membrane Proteomics Transporters Nitrogen sources Endocytosis Arrestin 
ProteomicsPlasma membrane proteomics
Aluminum (Al) toxicity in acid soil is a worldwide agricultural problem that inhibits crop growth and productivity. However, the signal pathways associated with Al tolerance in plants still remain largely unclear. In this study, tandem mass tag (TMT)-based quantitative proteomic methods was used to ...
ORGANISM(S): Glycine max 
2020-06-25 | PXD017160 | Pride
Aluminum resistance Plasma membrane Tmt Prm Tbs Proteomics 
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