Sort   by:  
 Page size 
ProteomicsCysteinome profiling of AgTU-induced cysteien oxidation (with TMT)
Apply a cysteine probe, DBIA, to profile AgTU-induced cysteine oxidation in HeLa cells
ORGANISM(S): Homo sapiens (Human) 
2024-03-17 | PXD043636 | Pride
Dbia probe Tmt Cysteine profiling 
ProteomicsCysteine profiling-abpp-label free quantification
Apply a cysteine probe, DBIA, to profile AgTU-induced cysteine oxidation in HeLa cells, quantified by LFQ
ORGANISM(S): Homo sapiens (Human) 
2024-03-17 | PXD048202 | Pride
Cysteine profiling Abpp Label free quantification 
ProteomicsChemoproteomic Profiling of Reactive Cysteines in Response to Oxidative Stress Induced by 6-Hydroxydopamine
Oxidative stress is a well-known driver of neurodegeneration, yet it remains poorly understood how the global cysteine (Cys) proteome is remodeled in such conditions. Proteins with aberrantly modified Cys in response to oxidative stress can induce and exacerbate neurodegeneration. In this study, we ...
ORGANISM(S): Homo sapiens (Human) 
2025-10-20 | PXD060980 | Pride
Quantitative proteomics Cell stress response Sh-sy5y Oxidative stress Neurodegeneration Neurodegenerative signaling 6-hydroxydopamine Cysteine proteomics 
ProteomicsReimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries
Current methods for measuring amino-acid side-chain reactivity lack the throughput needed to screen large chemical libraries for interactions across the proteome. Here we redesigned the workflow for activity-based protein profiling of reactive cysteine residues by using a smaller desthiobiotin-based...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-01-26 | PXD022511 | Pride
Reactive cysteines Chemical proteomics 
UnknownChemoproteomic Profiling of Reactive Cysteines in Response to Oxidative Stress Induced by 6-Hydroxydopamine.
Not available
S-EPMC12522117 | biostudies-literature
ProteomicsProteins modified by succination
Fumarate accumulation during renal fibrosis may be involved in renal inflammation by conferring succination to certain proteins to influence intracellular signaling. We applied a Desthiobiotin-Iodoacetamide (DBIA) probe as a competitive probe for covalently labelling fumarate and combined with a liq...
ORGANISM(S): Mus Musculus 
PXD063006 |
ProteomicsDevelopment of inhibitors, probes, and PROTAC provides a complete toolbox to study PARK7 in the living cell
The innovation of combined chemical tools, such as small molecule inhibitors, activity-based probes (ABPs), and proteolysis targeting chimeras (PROTACs) for a specific target, not only advances clinical drug discovery but also provides a chemical toolbox to study the diverse biological perspective o...
ORGANISM(S): Homo sapiens (Human) 
2024-06-16 | PXD047093 | Pride
Park7 Small molecule inhibitors Protacs Activity-based probes 
ProteomicsCellular Validation of Chemically Improved Inhibitor Identifies Monoubiquitination on OTUB2
Ubiquitin thioesterase OTUB2, a cysteine protease from the ovarian tumor (OTU) deubiquitinase superfamily, is associated with enhanced expression during tumor progression and metastasis. Development of OTUB2 inhibitors is therefore believed to be therapeutically important, yet potent and selective s...
ORGANISM(S): Homo sapiens (Human) 
2023-06-27 | PXD041346 | Pride
Otub2 Small-molecule inhibitor Monoubiquitination 
ProteomicsTMT-ABPP cysteine profiling of SETDB1 modulators in MCF7 native proteome
We prodiled the cystene engagement of SETDB1 modulators at 10 uM in native MCF7 proteome/lysate with or without spiked of recombinant SETDB1 proteins.
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD056190 | Pride
Abpp Cysteine Tmt Setdb1 
ProteomicsDrugMap: A quantitative pan-cancer analysis of cysteine ligandability
Cysteine-focused chemical proteomic platforms have accelerated the clinical development of covalent inhibitors of a wide-range of targets in cancer. However, how different oncogenic contexts influence cysteine targeting remains unknown. To address this question, we have developed DrugMap, an atlas o...
ORGANISM(S): Homo sapiens (Human) 
2024-04-23 | PXD047840 | Pride
Cysteine ligandability Quantitative proteomics Lc-ms/ms 
Sort   by:  
 Page size