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ProteomicsIntegrating serum protein electrophoresis with mass spectrometry, a new workflow for M-protein detection and quantification
Serum protein electrophoresis (SPE) and immunofixation electrophoresis (IFE) are standard tools for multiple myeloma (MM) routine diagnostics. M-protein is a biomarker for MM that can be quantified with SPE and characterized with IFE. We have investigated combining SPE/IFE with targeted mass spectro...
ORGANISM(S): Homo sapiens (Human) 
2020-01-07 | PXD016325 | Pride
Serum protein electrophoresis Immunofixation electrophoresis Targeted mass spectrometry Multiple myeloma M-protein 
ProteomicsIdentification of a collagen marker in urine improves the detection of colorectal liver metastases
Previously, we reported that a combination of a collagen alpha-1(I) natural occurring peptide (NOP) AGPP(-OH)GEAGKP(-OH)GEQGVP(-OH)GDLGAP(-OH)GP (AGP) and serum carcinoembryonic antigen (CEA) has the potential to detect colorectal liver metastases (CRLM). The combined method needs to be adapted to i...
ORGANISM(S): Homo sapiens (Human) 
2019-11-15 | PXD013533 | Pride
Logistic regression model Colon cancer Colorectal cancer Prm Collagen Colorectal liver metastases Mass spectrometry Natural occurring peptides Urine 
ProteomicsRapid detection of beta-lactam, aminoglycoside and fluoroquinolone resistance mechanisms in blood cultures positive for E. coli or K. pneumoniae using targeted LC-MS/MS
New and rapid antimicrobial susceptibility/resistance testing methods are required for bacteria from positive blood cultures. In the current study we developed and evaluated a targeted LC-MS/MS assay for the detection of beta-lactam, aminoglycoside and fluoroquinolone resistance mechanisms in blood ...
ORGANISM(S): Klebsiella pneumoniae JH1 Klebsiella variicola At-22 Acinetobacter sp. PM-14 Escherichia coli 
2022-07-01 | PXD025365 | Pride
Blood cultures Gyra E. coli Liquid chromatography-mass spectrometry Sepsis Qnr proteins Parallel reaction monitoring Aminoglycoside modifying enzymes K. pneumoniae 16s ribosomal rna methyltransferases Beta-lactamases Antimicrobial resistance 
ProteomicsIdentification of a collagen marker in urine improves the detection of colorectal liver metastases
Previously, we reported that a combination of a collagen alpha-1(I) natural occurring peptide (NOP) AGPP(-OH)GEAGKP(-OH)GEQGVP(-OH)GDLGAP(-OH)GP (AGP) and serum carcinoembryonic antigen (CEA) has the potential to detect colorectal liver metastases (CRLM). The combined method needs to be adapted to i...
ORGANISM(S): Homo sapiens (Human) 
2019-11-15 | PXD013705 | Pride
Logistic regression model Colon cancer Colorectal cancer Prm Collagen Colorectal liver metastases Mass spectrometry Natural occurring peptides Urine 
ProteomicsEffects of the IDH1 R132H mutation on the energy metabolism: a comparison between tissue and corresponding primary glioma cell cultures
The R132H mutation in the metabolic enzyme isocitrate dehydrogenase 1 (IDH1) is the most important prognostic factor for survival of glioma patients. This resulted in many studies investigating the effects of this mutation, including those on energy metabolism. This led to the discovery of a panel o...
ORGANISM(S): Homo sapiens (Human) 
2022-02-16 | PXD024019 | Pride
Idh1 r132h mutation Cell cultures Glioma Proteomics Energy metabolism 
ProteomicsRapid and accurate detection of aminoglycoside modifying enzymes and 16S ribosomal RNA methyltransferases by targeted LC-MS/MS
New and rapid diagnostic methods are needed for the detection of antimicrobial resistance to aid in the curbing of drug-resistant infections. Targeted LC-MS/MS is a method that could serve this purpose, as it can detect specific peptides of resistance mechanisms with high accuracy. In the current st...
ORGANISM(S): Escherichia coli Klebsiella pneumoniae BIDMC 40 
2021-04-30 | PXD024339 | Pride
E. coli Aminoglycosides Parallel reaction monitoring Aminoglycoside modifying enzymes Liquid chromatography mass spectrometry K. pneumoniae 16s ribosomal rna methyltransferases Antimicrobial resistance 
ProteomicsLC-MS/MS analysis demonstrates a decrease in porins and increase in CMY-2-like beta-lactamases in E. coli exposed to meropenem
While ESBL and AmpC beta-lactamases barely degrade carbapenems, they are able to bind them and prevent them from interacting with penicillin binding proteins thereby preventing their effect. When these beta-lactamases are expressed at a high level and combined with a decreased influx of carbapenems ...
ORGANISM(S): Escherichia coli 
2022-04-04 | PXD025363 | Pride
E. coli Cmy-2-like Liquid chromatography-mass spectrometry Ompc Meropenem Parallel reaction monitoring Chromosomal ampc Ompf Antimicrobial resistance 
ProteomicsA systematic exploration of antimicrobial resistance to beta-lactams, aminoglycosides and fluoroquinolones in Escherichia coli and Klebsiella pneumoniae using proteogenomics
Background: Antimicrobial resistance is generally studied using a combination of growth inhibition measurements, sometimes in combination with DNA detection methods. However, the actual proteins that cause resistance such as enzymes, efflux pumps and a lack of porins cannot be detected by these meth...
ORGANISM(S): Escherichia coli 
2021-07-09 | PXD023736 | Pride
Liquid chromatography Mass spectrometry Aminoglycosides K. pneumoniae Data dependent acquisition Porins Comprehensive antibiotic resistance database Beta-lactams Fluoroquinolones Minimum inhibitory concentrations E. coli Whole genome sequencing Antimicrobial resistance 
ProteomicsA systematic exploration of antimicrobial resistance to beta-lactams, aminoglycosides and fluoroquinolones in Escherichia coli and Klebsiella pneumoniae using proteogenomics
Background: Antimicrobial resistance is generally studied using a combination of growth inhibition measurements, sometimes in combination with DNA detection methods. However, the actual proteins that cause resistance such as enzymes, efflux pumps and a lack of porins cannot be detected by these meth...
ORGANISM(S): Klebsiella pneumoniae 1162281 
2021-07-09 | PXD023739 | Pride
Liquid chromatography Mass spectrometry Aminoglycosides K. pneumoniae Data dependent acquisition Porins Comprehensive antibiotic resistance database Beta-lactams Fluoroquinolones Minimum inhibitory concentrations E. coli Whole genome sequencing Antimicrobial resistance 
ProteomicsIdentification of barrier-specific proteins in the human brain
We present a proteomic analysis of the basal lamina of the human BBB. We combined laser capture microdissection with shotgun proteomics for selective enrichment and identification of specific proteins present in the cerebral microvasculature and arachnoidal vessels collected from normal human brain ...
ORGANISM(S): Homo sapiens (Human) 
2022-01-17 | PXD018602 | Pride
Blood-brain barrier Proteomics Mass spectrometry 
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