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MetabolomicsDynamic utilization of low-molecular-weight organic substrates across a microbial growth rate gradient

AIM: Low-molecular-weight organic substances (LMWOSs) are at the nexus between micro-organisms, plant roots, detritus and the soil mineral matrix. The nominal oxidation state of carbon (NOSC) has been suggested as a potential parameter for modelling microbial uptake rates of LMWO...

2023-08-14 | MTBLS3558 | MetaboLights
TranscriptomicsFluidigm Biomark 96.96 nanofluidic arrays for qRT-PCR of 93 miRNAs: salivary extracellular vesicles
Crude extracellular vesicles (EVs) from eight healthy volunteers were separated into 6 fractions based on their densities by using the iodixanol-based density gradient centrifugation method. To determine the distribution of miRNAs among these fractions, quantities of 93 miRNAs were quantified by the...
ORGANISM(S): Homo sapiens 
2016-08-01 | E-GEOD-78763 | biostudies-arrayexpress
TranscriptomicsImmune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube and standard density gradient
High quality genetic material is an essential pre-requisite when analyzing gene expression using microarray technology. Peripheral blood mononuclear cells (PBMC) are frequently used for genomic analyses, but several factors can affect the integrity of nucleic acids prior to their extraction, includi...
ORGANISM(S): Homo sapiens 
2015-08-28 | E-GEOD-67321 | biostudies-arrayexpress
ProteomicsIn-depth protein profiling of mouse hippocampal postsynaptic density using data-independent acquisition proteomics
In the present study, we catalogued the proteome of postsynaptic density (PSD) derived from the hippocampus of three-week old (P19) mice. samples in 100 min gradient time.
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2019-08-27 | MSV000084240 | MassIVE
ProteomicsThe concept of "RNA dependence" - Proteome-wide and quantitative identification of protein interactions dependent on RNA
The general role of RNA in the cell beyond protein biosynthesis is only beginning to emerge with little knowledge about the structural or organizational functions of RNA. In turn, functional non-coding RNAs (ncRNAs) are often identified by their regulation or impact on cellular phenotypes. However, ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-06-13 | MSV000082468 | MassIVE
TranscriptomicsDensity Gradients Separate Dormant and Treatment-Resistant Human Glioblastoma Cells
Glioblastoma (GBM) is among the most aggressive cancers. Despite aggressive radiotherapy and treatment with the alkylating agent temozolomide (TMZ), patients ultimately succumb to the disease. Although much interest has focused on highly tumorigenic GBM stem cells (GSCs), adaption of a concept from ...
ORGANISM(S): Homo sapiens 
2016-07-01 | E-GEOD-71116 | biostudies-arrayexpress
ProteomicsCardiac Atrial Compartmentalisation Proteomics: A Modified Density Gradient Method to Analyse Endo-lysosomal Proteins
Evidence that lysosomes are important in cardiac physiology and pathology is now emerging. We describe a label-free method suitable for small cardiac tissue biopsies based on a density-separated fractionation protocol, which allows us to study endo-lysosomal (EL) proteins in guinea pig atria. The t...
ORGANISM(S): Cavia porcellus (Guinea pig) 
2021-09-09 | PXD021277 | Pride
Cardiac Rab proteins Lysosome Guinea-pig Lysosome storage disease Atria 
ProteomicsComparison of purified membrane vesicles from L. casei submitted or not to density gradient ultracentrifugation
Probiotics are defined by the World Health Organization as “live microorganisms which, when administered in adequate amounts confer a health benefit on the host”. A large number of probiotic bacteria used in the food industry are members of the diverse Lactobacillaceae family and are “Generally Rega...
ORGANISM(S): Lacticaseibacillus casei BL23 
2023-01-25 | PXD036342 | Pride
Membrane vesicles Density gradient Lacticaseibacillus casei Nanolc-ms/ms 
ProteomicsHuman Cardiovascular Tissue Extracellular Vesicles – Density Gradient
Fewer than 50% of patients develop vascular and valvular calcification, implying differential pathogenesis. Tissue-entrapped extracellular vesicles (EVs) are found in mineralization but their contents and functions are unstudied. Tissue EVs were isolated from diseased human carotid arteries and aort...
ORGANISM(S): Hathewaya proteolytica Homo sapiens (Human) HATHI 
2023-07-10 | PXD035580 | Pride
Human Lc-msms Extracellular vesicle Exosome Calcification Aortic valve Carotid endarterectomy Atherosclerotic plaque 
ProteomicsProteomic Analysis of the Protein Composition of Migrasomes Enriched by Magnetic Aptamer Probe System and Density Gradient Centrifugation
This study aims to evaluate the performance of the magnetic aptamer probe (MAP) system in the enrichment of migrasomes. First, the MAP system was constructed by functionalizing biotin-labeled migrasome-targeting aptamers (Apt_B3) onto the surface of NeutrAvidin-coated magnetic sub-micrometre particl...
ORGANISM(S): Homo sapiens (Human) 
2026-04-27 | PXD074521 | Pride
Density gradient centrifugation Proteomic Aptamer Migrasome 
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