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ProteomicsThe Polycomb group protein CBX6 is an essential regulator of embryonic stem cell identity
Polycomb group proteins are transcriptional repressors that control cell identity and development. In mammals, at least five different CBX proteins are believed to associate with the core Polycomb repressive complex 1 (PRC1). CBX6 and CBX7 are the most highly expressed CBX proteins in embryonic stem...
ORGANISM(S): Mus musculus (Mouse) 
2017-09-25 | PXD007577 | Pride
Mouse Pluripotency Esc Affinity capture followed by ms Cbx6 
ProteomicsSUZ12 immunoprecipitation followed by PRM of SUZ12 peptides to identify alternatively spliced isoforms of SUZ12 at the protein level.
Alternative splicing of SUZ12 exon 4 is predicted to generate two alternative isoforms differing by the inclusion or exclusion of 69 nucleotides in exon 4, which we named SUZ12-long (SUZ12-L) and SUZ12-short (SUZ12-S) respectively. At the protein level SUZ12 exon 4 encodes 23 amino acids (aa 129–152...
ORGANISM(S): Mus musculus (Mouse) 
2024-03-18 | PXD046742 | Pride
Exon As Ip Prc2 Mescs Alternative splicing Prm. Escs Suz12 Polycomb 
ProteomicsSUZ12 immunoprecipitation followed by protein indentification via mass spectrometry (SUZ12 IP-MS) in mESCs of SUZ12 WT, delta exon 4, KO, and KO rescues with SUZ12-L and SUZ12-S
Suz12 exon 4 encodes 23 amino acids (aa 129–152 in SUZ12-L) that partially overlap with the WD-binding domain 1 (WDB1, 110–145). We reasoned that exon 4 skipping might alter the structure of SUZ12 and, possibly, PRC2 composition. To explore this possibility, we generated ESCs that lack the Suz12 exo...
ORGANISM(S): Mus musculus (Mouse) 
2024-03-18 | PXD046660 | Pride
Flag-tag Rescues Ko Ip Prc2 Interactions Mescs Escs Suz12 Polycomb 
TranscriptomicsHuman NTERA2 (NT2/D1) cells lines
This SuperSeries is composed of the following subset Series: GSE16858: Human NTERA2 (NT2/D1) cells lines: shZRF1 vs. shRandom during Retinoic Acid (RA) administration GSE16878: Human NTERA2 (NT2/D1) cells lines: Genomewide distribution of ZRF1 at gene promoters during RA administration Refer to indi...
ORGANISM(S): Homo sapiens 
2010-12-24 | E-GEOD-16891 | biostudies-arrayexpress
TranscriptomicsNon-Overlapping Functions of the Polycomb group Cbx Family of Proteins in Embryonic Stem Cells
Gene expression profile in control and Cbx7 depleted (shRNA#33) mouse embryonic stem cells and embryoid bodies at day 9 4 samples, each one contains four biological replicas, except for ES-shCtr which contains 3 replicas
ORGANISM(S): Mus musculus 
2012-01-06 | E-GEOD-34191 | biostudies-arrayexpress
GenomicsZrf1 is required to establish and maintain neural progenitor identity (ChIP-Seq)
We report ChIP-seq data for Zrf1 and Ring1B occupancy in NPC Examination and comparison of DNA binding profile of Zrf1 and Ring1B in NPC
ORGANISM(S): Mus musculus 
2013-12-21 | E-GEOD-53540 | biostudies-arrayexpress
TranscriptomicsGene regulation by phosphomimetic Myc (MycD), cytosine arabinoside (AraC) and retinoic acid (RA)
We aimed to investigate whether the previously observed synergistic effects of AraC and RA in stimulating leukemia differentiation could be at least partially dependent on Pak2-mediated phosphorylation of Myc. Genome-wide analysis of NB4 cells treated with RA, AraC with RA, or MycD with RA revealed ...
ORGANISM(S): Homo sapiens 
2012-01-05 | E-GEOD-29213 | biostudies-arrayexpress
ProteomicsAP-MS of EPOP in mouse embryonic stem cells
A Flag-tagged version of murine EPOP was expressed in mouse embryonic stem cells (ESCs) at near to endogenous levels and purified using an anti-FLAG affinity matrix.
ORGANISM(S): Mus musculus (Mouse) 
2016-12-13 | PXD005082 | Pride
Esc Prc2 Polycomb 
ProteomicsAP-MS of Elongin B in mouse embryonic stem cells
A Flag-tagged version of murine Elongin B was expressed in mouse embryonic stem cells (ESCs) at near to endogenous levels and purified using an anti-FLAG affinity matrix.
ORGANISM(S): Mus musculus (Mouse) 
2016-12-13 | PXD005081 | Pride
Esc Elob Prc2 Tceb2 Polycomb 
TranscriptomicsGene regulation by phosphomimetic Myc and retinoic acid
We aimed to investigate the E-box and Max-independent functions of the oncogene Myc in gene regulation and differentiation of leukemic cells. Expression in HL60 leukemic cells of a mutant form of Myc with impaired Max and E-box interaction by replacing the phosphorylation sites of Pak2 kinase to asp...
ORGANISM(S): Homo sapiens 
2012-01-05 | E-GEOD-24731 | biostudies-arrayexpress
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