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The S831 phosphosite on AFF4 lies within a disordered region adjacent to the ENL/AF9 binding site. We therefore hypothesized that phosphorylation may regulate protein:protein interactions within the Super Elongation Complex. To investigate this, HEK293T cells expressing RFP negative control, FLAG-ta...
ORGANISM(S): Homo sapiens (Human) 
2025-12-16 | PXD054250 | Pride
Aff4 was recently identified as an insulin-regulated phosphoprotein. To investigate upstream kinases, we analysed immunoprecipitated Aff4 treated with our without insulin and treated with either an Akti or a P70S6Ki. These results showed that AFF4 is dependent on P70S6K and therefore an in vitro kin...
ORGANISM(S): Mus musculus (Mouse) 
2025-12-16 | PXD054247 | Pride
We compared the in vivo insulin signaling response in bone tissue of 10- verses 73-week old C57BL/6J male mice (Figure 1A). Saline or insulin (2.5 mU/kg) was interperitoneally injected and mice sacrificed after 20 min. Tibia bone was rapidly dissected and flushed with ice-cold PBS within 30 sec to r...
ORGANISM(S): Mus musculus (Mouse) 
2025-12-16 | PXD054205 | Pride
Knocked-down of P70S6K in zebrafish (double knockdown of rps6kb1/2) using embryo Cas9/gRNA RNP injections followed by phosphoproteomics of caudal fin.
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2025-12-16 | PXD054212 | Pride
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