Sort   by:  
 Page size 
ProteomicsPerchlorate-Specific Proteomic Stress Responses of Debaryomyces hansenii Could Enable Microbial Survival in Martian Brines
If life exists on Mars, it would face several challenges including the presence of perchlorates, which destabilize biomacromolecules by inducing chaotropic stress. However, little is known about perchlorate toxicity for microorganism on the cellular level. Here we present a proteomic investigation o...
ORGANISM(S): Debaryomyces hansenii 
2023-06-06 | PXD033237 | Pride
Debaryomyces hansenii Astrobiology Mars Martian brines Perchlorate Stress response 
ProteomicsIncreasing proteome depth while maintaining quantitative precision in short gradient data-independent acquisition proteomics
The combination of short liquid chromatography (LC) gradients and data independent acquisition (DIA) by mass spectrometry (MS) has proven its huge potential for high-throughput proteomics. However, the optimization of isolation window schemes resulting in a certain number of data points per peak (DP...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2023-05-10 | PXD036451 | Pride
Isolation windows Dia Multi-species Short-gradient 
ProteomicsTriggered offset fragmentation allows massively multiplexed target profiling on quadrupole-orbitrap mass spectrometers
Parallel reaction monitoring (PRM) on quadrupole-orbitrap mass spectrometers has a limited multiplexing capacity which depends heavily on the reproducibility of peptide retention times. To overcome these limitations, we aimed to establish a data acquisition mode that allows retention-time-independen...
ORGANISM(S): Candida albicans (Yeast) Homo sapiens (Human) 
2020-06-19 | PXD017778 | Pride
Prm Multiplexing Targeted proteomics Quantification Lc-ms/ms 
ProteomicsDirect translation of incoming retroviral genomes
Viruses that carry a positive-sense, single-stranded (+ssRNA) RNA translate their genomes soon after entering the host cell to produce viral proteins, with the exception of retroviruses. A distinguishing feature of retroviruses is reverse transcription, where the +ssRNA genome serves as a template t...
ORGANISM(S): Homo sapiens (Human) Human immunodeficiency virus 1 
2024-01-26 | PXD046777 | Pride
Hiv Silac Ip-ms 
ProteomicsSurviving Martian Brines: Proteomic Analysis of Escherichia coli’s Adaptation to Perchlorate Stress
Brines, potentially formed by the deliquescence and freezing point depression of highly hygroscopic salts, such as perchlorates (ClO4-), may allow for the spatial and temporal stability of liquid water on present-day Mars. It is therefore of great interest to explore the microbial habitability of Ma...
ORGANISM(S): Escherichia coli 
2025-01-21 | PXD055273 | Pride
Mars Adaptation Astrobiology Brines Perchlorate Habitability 
ProteomicsSample Preparation by Easy Extraction and Digestion (SPEED) - A Universal, Rapid, and Detergent-free Protocol for Proteomics based on Acid Extraction
We report a universal detergent-free method, named Sample Preparation by Easy Extraction and Digestion (SPEED), which consists of three mandatory steps, acidification, neutralization and digestion. SPEED is a universal method for peptide generation from various sources and is easily applicable even ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) Bacillus cereus ATCC 10987 Escherichia coli Staphylococcus aureus 
2019-11-25 | PXD011189 | Pride
Speed Sample preparation Quantitative proteomics 
ProteomicsComparison of the Cowpox Virus and Vaccinia Virus Mature Virion Proteome: Analysis of the species- and strain-specific Proteome
Cowpox virus (CPXV) causes most zoonotic orthopoxvirus (OPV) infections in Europe and Northern as well as Central Asia. The virus has the broadest host range of OPV and is transmitted to humans from rodents and other wild or domestic animals. Increasing numbers of human CPXV infections in a populati...
ORGANISM(S): Orthopoxvirus 
2015-11-16 | PXD003013 | Pride
Ltq-orbitrap Virion proteome Orthopoxvirus 
ProteomicsvPro-MS enables identification of human-pathogenic viruses from patient samples by untargeted proteomics
Viral infections are commonly diagnosed by the detection of viral genome fragments or proteins using targeted methods such as PCR and immunoassays. In contrast, metagenomics enables the untargeted identification of viral genomes, expanding its applicability across a broader spectrum. In this study, ...
ORGANISM(S): Homo sapiens (Human) 
2025-08-04 | PXD055135 | Pride
Pathogen detection Virus identification Virome Virus 
ProteomicsProteomic identification of a Toxoplasma gondii sporozoite-specific antigen using HDAC3 inhibitor-treated tachyzoites as surrogate
The apicomplexan parasite Toxoplasma gondii has a complex life cycle involving multiple host species. Access to sexual stages and sporozoite-containing oocysts, essential for the parasite’s environmental transmission, is limited and requires animal experiments with cats. In vitro alternatives and re...
ORGANISM(S): Toxoplasma gondii Homo sapiens (Human) 
2025-05-07 | PXD055279 | Pride
Toxoplasma gondii Protein-protein interaction Ip-ms 
ProteomicsSilence as a way of niche adaptation: mecC-MRSA with variations in the accessory gene regulator (agr) functionality express kaleidoscopic phenotypes
Functionality of the accessory gene regulator (agr) quorum sensing system is an important switch promoting either acute or chronic infections, mediated by the notorious opportunistic human and veterinary pathogen Staphylococcus aureus. Spontaneous alterations of the agr system are known to frequentl...
ORGANISM(S): Staphylococcus aureus 
2021-09-09 | PXD016486 | Pride
Bacteria Mecc-mrsa S. aureus 
Sort   by:  
 Page size