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ProteomicsEndo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons
We developed a toolkit for capturing endosomes (Endo-IP) and lysosomes (Lyso-IP) in human stem cell(hESC)-derived induced neurons (iNeurons). We target a sub-population of early endosomes marked by endogenously tagged EEA1 (Early Endosome Antigen 1) protein, which represents one of the earliest popu...
ORGANISM(S): Homo sapiens (Human) 
2025-01-11 | PXD055978 | Pride
Human Tmt Endosomes Ineurons Lysosomes 
UnknownEndo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons.
Not available
S-EPMC11463543 | biostudies-literature
UnknownEndo-IP and lyso-IP toolkit for endolysosomal profiling of human-induced neurons.
Not available
S-EPMC11670117 | biostudies-literature
ProteomicsCharacterization of endosomes and interactions from TMEM230 and TMEM9/9B mutants in human neurons
EndoMAP aims to systematically dissect the human endosomal protein complexes. To examine TMEM9 and TMEM230 interactions, we generated TMEM230 and TMEM9 knockouts (KOs) using CRISPR/Cas9 in human embryonic stem (ES) cells and converted the cells to induced neurons (iNeurons). TMEM230 and TMEM9 were i...
ORGANISM(S): Homo sapiens (Human) 
2025-03-29 | PXD054765 | Pride
Tmt Human Tmem9 Endosomes Ineurons Tmem230 Endomap 
ProteomicsBlue Native PAGE co-fractionation of purified human endosomes
EndoMAP aims to systematically dissect the human endosomal protein complexes. To purify endosomes from human cells, a modified Endo-IP protocol was developed to be compatible with complexomics methodologies and evaluated by DIA-MS. Three independent biological replicates of human endosomes purified ...
ORGANISM(S): Homo sapiens (Human) 
2025-03-29 | PXD054684 | Pride
Co-fractionation Human Endosomes Bn-page Endomap 
UnknownAutocrine A2 in the T-system of ventricular myocytes creates transmural gradients in ion transport: a mechanism to match contraction with load?
Not available
S-EPMC4052267 | biostudies-literature
TranscriptomicsC. elegans PRG-1 co-IP small RNAs, 5p-end-independent
These datasets examine the association of small RNAs with 3p hydroxyls (including endo-siRNAs, miRNAs, and 21U-RNAs) with the PRG-1 protein through co-immunoprecipitation. Two datasets are provided; one sequences small RNAs that co-IP with PRG-1; the other sequences small RNAs from the IP input samp...
ORGANISM(S): Caenorhabditis elegans 
2008-06-16 | GSE11737 | GEO
UnknownSpatial snapshots of amyloid precursor protein intramembrane processing via early endosome proteomics.
Not available
S-EPMC9573879 | biostudies-literature
UnknownThe IP3 receptor regulates cardiac hypertrophy in response to select stimuli.
Not available
S-EPMC2933281 | biostudies-literature
TranscriptomicsExpression data from from the E9.25 hearts of mouse embryos
Congenital heart defects (CHDs) occur in 0.5–1% of live births, yet the underlying genetic etiology remains mostly unknown. Recently, a new source of myocardial cells, namely the second heart field (SHF), was discovered in the splanchnic mesoderm. Abnormal development of the SHF leads to a spectrum ...
ORGANISM(S): Mus musculus 
2011-03-26 | E-GEOD-28186 | biostudies-arrayexpress
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