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ProteomicsCell-type specific tagging of glycoproteins in murine-human co-culture samples
We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK/Nh-GalNAc-T2) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected ce...
ORGANISM(S): Homo sapiens (Human) 
2022-09-10 | PXD035438 | Pride
Mcf7 Co-culture  lfq Proteomics Human-murine 4t1 Secretome 
ProteomicsCell-type specific tagging of glycoproteins in murine-human co-culture secretome glycoproteomics
We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK/Nh-GalNAc-T2) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected ce...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-09-10 | PXD035445 | Pride
Mcf7 Human-murine 4t1 Secretome Co-culture  glycoproteomics 
ProteomicsCell-type specific tagging of glycoproteins in murine-human co-culture samples glycoproteomics lysates
We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK/Nh-GalNAc-T2) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected ce...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-09-10 | PXD035449 | Pride
Mcf7 Human-murine Lysate 4t1 Co-culture  glycoproteomics 
ProteomicsCell-type specific labelling of O-linked glycoproteins in engineered K-562 cells
We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected cells incorpora...
ORGANISM(S): Homo sapiens (Human) 
2022-09-10 | PXD035437 | Pride
Human Mono-culture Silac Whole lysates K-562 
ProteomicsCell-type specific labelling of glycoproteins in engineered K-562 cells (BOCTAG)
We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected cells incorpora...
ORGANISM(S): Homo sapiens (Human) 
2022-09-10 | PXD035430 | Pride
Human Mono-culture Silac Whole lysates K-562 
ProteomicsHepG2 secretome tagged with the sugar analogs GalNAz and GalNAzMe
Chemically modified analogs of GalNAc, termed GalNAz or GalNAzMe, were incorporated in secreted glycoproteins. The compounds contain bioorthogonal, clickable functional groups that allowed for enrichment using biotin. Chemical glycoproteomics was performed after on-bead digestion and cleavage of gly...
ORGANISM(S): Homo sapiens (Human) 
2020-09-14 | PXD020648 | Pride
Glycosylation Bioorthogonal Mucin Chemical proteomics Enrichment Biotin 
UnknownResults of the two incidence screenings in the National Lung Screening Trial.
Not available
S-EPMC4307922 | biostudies-literature
UnknownThe National Lung Screening Trial: overview and study design.
Not available
S-EPMC3009383 | biostudies-literature
UnknownReduced lung-cancer mortality with low-dose computed tomographic screening.
Not available
S-EPMC4356534 | biostudies-literature
UnknownAcute rehabilitation following traumatic anterior shoulder dislocation (ARTISAN): pragmatic, multicentre, randomised controlled trial.
Not available
S-EPMC10792684 | biostudies-literature
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