Study of EVs from CNS tissues becomes challenging with the current available methods of EVs enrichment. We recently developed PROSPR, a standardized and easy-to-perform method to enrich EVs from biological fluids. We have optimized the use of PROSPR to efficiently enrich EVs from CNS tissues.
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human)
In this study we have profiled the temporal lobe proteome of AD+CVD subjects and age-matched controls. We have studied the occurrence of uDPMs in the particulate and soluble brain proteomes isolated and analyzed by ultracentrifugation-electrostatic repulsion hydrophilic interaction chromatography (U...
In this project we provide profiling of brain-derived and circulating EVs LC-MS/MS data from mice subjected to bilateral common carotid stenosis (BCAS) and Sham controls. Furthermore, data from brain-derived EVs of early AD and mixed dementias (AD+CVD) is also provided.
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse)
Here, we report a novel and inexpensive method of rapidly isolating EVs from small volumes of human blood plasma by PRotein Organic Solvent PRecipitation (PROSPR). PROSPR encompasses a rapid three-step protocol to remove soluble proteins from plasma via precipitation in cold acetone, leaving the lip...
Unbiased discovery-driven quantitative proteomics was performed to profile expression changes and degenerative modifications in the temporal lobe proteome (BA21) of AD+CVD patients compared to normal age-matched controls.
Data from ProteomeXchange, PXD ID: PXD002668. File: UltraCushion_R1_28.mzml. Published as part of Sci Rep. 2015 Sep 30;5:14664 . From the Abstract: {{i}} Extracellular vesicles (EVs) such as exosomes and microvesicles mediate intercellular communication and regulate a diverse range of crucial biolo...
Extracellular vesicles (EVs) constitute an intricate system of molecular exchange that has recently gained tremendous interest. However, sustainable sources of safe biological EVs remain scarce and elusive. In this study we explore and define the use of food industry byproducts (BP) as a circular...
