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ProteomicsTalin1-GFP pulldown in U2OS cells
In this study, we pull-down human Talin1-GFP and GFP from U2OS cells plated on fibronectin. Binding partners were then analysed using mass-spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2021-05-12 | PXD024634 | Pride
Talin1 Gfp trap U2os 
ProteomicsTLNRD1-GFP pulldown in HEK cells
In this study, we pull-down human TLNRD1-GFP and GFP from HEK cells. Binding partners were then analysed using mass-spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2024-01-27 | PXD045258 | Pride
Cell lines Gfp trap 
ProteomicsGFP pulldown for the RiboVD project
GFP-tagged viral peptide sequences and their controls were used to pull down interactors from human (uninfected) or green monkey (viral infected) cell lysate. After sample preparation, digestion and bottom-up LC-MS/MS analysis, the enriched proteins were identified.
ORGANISM(S): Homo sapiens (Human) Cercopithecus aethiops (Green monkey) (Grivet) 
2023-03-09 | PXD033874 | Pride
Pulldown Motif Virus Interaction Coronavirus 
ProteomicsIdentification of ESAG4-interacting proteins in Trypanosoma brucei bloodstream forms
How flagellar signaling regulates the host interaction of parasites remains a challenge due to poor conservation of signaling systems with those in cilia of higher organisms. Flagellar cAMP signaling is implicated in interaction of the unicellular parasite Trypanosoma brucei with its hosts. The seco...
ORGANISM(S): Trypanosoma brucei brucei 
2022-08-31 | PXD025412 | Pride
Adenylate cyclase Cyclase-interacting protein Camp signaling Gfp trap 
ProteomicsPVY Coat Protein Interactome - In order to identify cellular proteins interacting with the coat protein (CP) of Potato virus Y (PVY), we used coimmunoprecipitation of GFP-CP in PVY-infected Nicotiana benthamiana plants. To be able to pull down non-abundant interactors and to stabilize transient interactions, we used the crosslinker dithiobis(succinimidyl propionate) (DSP). With this we were able to identify a total of 147 potential CP interactors.
In order to identify cellular proteins interacting with the coat protein (CP) of Potato virus Y (PVY), we used coimmunoprecipitation of GFP-CP in PVY-infected Nicotiana benthamiana plants. To be able to pull down non-abundant interactors and to stabilize transient interactions, we used the crosslink...
ORGANISM(S): Nicotiana benthamiana ssRNA viruses 
2017-12-18 | PXD007836 | Pride
Coat protein Crosslinking Pvy Potato virus y Cp Nicotiana benthamiana Dsp 
ProteomicsMass spectrometry hits for GFP::BBLN-1/bioGFP::BBLN1 pull down
Epithelial tubes are essential components of metazoan organ systems that control the flow of fluids and the exchange of materials between body compartments and the outside environment. The size and shape of the central lumen confer important characteristics to tubular organs and need to be carefully...
ORGANISM(S): Homo sapiens (Human) Caenorhabditis elegans 
2021-04-07 | PXD024387 | Pride
C. elegans Bublin Affinity purification Mass spectrometry Bbln-1 Hek293t 
ProteomicsIdentification of CARP3-interacting proteins in Trypanosoma brucei bloodstream forms and procyclic forms
How flagellar signaling regulates the host interaction of parasites remains a challenge due to poor conservation of signaling systems with those in cilia of higher organisms. The trypanosome-specific cAMP response protein 3 (CARP3) shows developmentally regulated localization at the flagellar tip me...
ORGANISM(S): Trypanosoma brucei brucei 
2022-08-31 | PXD025398 | Pride
Cyclase-interacting protein Adenylate cyclase Carp3 Camp response protein Gfp trap 
ProteomicsTurboID proximity labelling with protein TRIM52: Identification of biotinylated proteins through pull-down and label-free quantification
All constructs were expressed in RKO cells (human rectal carcinoma cell line). A fusion construct of TurboID (mutated BirA* from E. Coli) and green fluorescent protein (GFP) or human TRIM52. Cells were stimulated with Epoxomicin (proteasome inhibitor) or left untreated. Biotinylated proteins were e...
ORGANISM(S): Homo sapiens (Human) 
2025-03-25 | PXD051272 | Pride
Trim52 Proximity labelling Turboid 
ProteomicsSvf1-GFP pulldowns from Limar et al. 2023 JCB
Svf1-GFP pulldowns compared to untegged WT cells from SILAC labelled yeast cells. See Limar et al. 2023 JCB
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-03-06 | PXD039395 | Pride
Svf1-gfp pulldowns from yeast svf1 
ProteomicsNUP153 interactome in human infected/uninfected
GFP-tagged NUP153 and suitable controls were used to pull down interactors from human cell lysate, where the cells were uninfected/infected with TBEV/infected with LGTV. After sample preparation, digestion and bottom-up LC-MS/MS analysis, the enriched proteins were identified.
ORGANISM(S): Homo sapiens (Human) 
2026-03-10 | PXD052523 | Pride
Tbev Nup153 Lgtv 
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