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ProteomicsComplementary proteomics strategies identify the ataxin-1 interactome in Neuro-2a cells
To identify protein partners of ataxin-1 in neuronal cells under control or stress conditions, we use complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-trap pulldown) in Neuro-2a cells expressing epitope-tagged forms of ataxin...
ORGANISM(S): Mus musculus (Mouse) 
2018-10-18 | PXD010352 | Pride
Gfp-trap Ataxin-1 Bioid Interactome Neuro-2a Lc – msms 
ProteomicsTalin1-GFP pulldown in U2OS cells
In this study, we pull-down human Talin1-GFP and GFP from U2OS cells plated on fibronectin. Binding partners were then analysed using mass-spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2021-05-12 | PXD024634 | Pride
Talin1 Gfp trap U2os 
ProteomicsTLNRD1-GFP pulldown in HEK cells
In this study, we pull-down human TLNRD1-GFP and GFP from HEK cells. Binding partners were then analysed using mass-spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2024-01-27 | PXD045258 | Pride
Cell lines Gfp trap 
TranscriptomicsRNA-seq and TRAP-seq of astrocytes and brain tissue in mouse models of Alzheimer's pathology
Alzheimer’s disease is known to alter astrocytes, but the effect of Aß and Tau pathology on these cells remains poorly understood. We investigated the transcriptomic behaviour of astrocytes (via translating ribosome affinity purification (TRAP)), and bulk brain tissue, in mouse models of APP/PS1 ß-a...
ORGANISM(S): Mus musculus 
2021-12-02 | E-MTAB-10985 | biostudies-arrayexpress
ProteomicsIP-MS of GFP tagged MRV μ2 protein
To determine which cellular proteins interacts with the viral MRV protein μ2 and its mutant μ2-P208S, GFP-tagged constructions of μ2 and μ2-P208S in both C- and N- terminus were transfected in 293T cells alongside control GFP alone for 24h, and submitted to GFP pulldown before IP were prepared for M...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD029701 | Pride
Virus ip-ms ap-ms gfp mrv reovirus μ2 
ProteomicsPianalto KM et al "Characterization of novel components of the environmental pH-sensing complex in Cryptococcus neoformans"
Three analyses performed by the Duke Proteomics and Metabolomics Shared Resource in support of the project entitled "". Sample ID21519 was called Rra1-GFP, a 1D coomassie-stained gel band from a GFP-Trap pulldown of Rra1-GFP from the membrane protein fraction of Cryptococcus neoformans. Sample ID21...
ORGANISM(S): Cryptococcus Neoformans (ncbitaxon:5207) 
2018-03-06 | MSV000082127 | MassIVE
ProteomicsPlasmodium falciparum bromo-domain containing epigenetic protein complexes recruited to modified histone tails as identified by histone peptide pulldown (HPP)-MS/MS and further characterized by GFP-IP-MS/MS and HA-IP-MS/MS
In order to identify epigenetic protein complexes recruited to histone PTMs in the deadly human malaria parasite Plasmodium falciparum, we set out to perform histone peptide pulldowns with various histone peptides. Biotinylated peptides corresponding to P. falciparum histone variant H2A.Z, H2B.Z and...
ORGANISM(S): Plasmodium falciparum 
2019-10-15 | PXD010644 | Pride
Epigenetic complex Bromo-domain protein Hpp Gcn5 Gfp-ip Histone peptide pulldown Interactor Plasmodium falciparum Taf1 Histone acetylation Ha-ip Reader complex Histone methylation 
ProteomicsGFP Pulldown from A375 cells expression microprotein-GFP candidates
Bottom-up mass spectrometric identification of interaction partners of candidate microproteins-GFP fusions after anti-GFP pulldown and tryptic digest Sample preparation For each of the conditions, we prepared two biological replicates. For each replicate, a confluent T75 flask of cells was pelleted...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-05-12 | MSV000091927 | MassIVE
ProteomicsInteracting partners of Rac1 (P29S/Q61L) compared to constitutively active Rac1 (Q61L)
Interactors of double mutant Rac1 (P29S/Q61L) compared to constitutively active Rac1 (Q61L).
ORGANISM(S): Chlorocebus sabaeus 
2019-11-12 | PXD013941 | Pride
Cos7 cells Rac1 Gfp trap pulldown 
ProteomicsSam68 interactome in mouse embryonic stem cells (mESCs)
Pellets from a mESC line with GFP tagged Sam68 and a control line (mESC expressing GFP alone) were collected at different time points during differentiation (Day 0, Day 3, Day 10).Pull-down was performed from normalized total cell lysates with GFP-trap beads (Chromotek) and analyzed by mass spectrom...
ORGANISM(S): Mus musculus (Mouse) 
2026-06-08 | PXD009209 | Pride
Rna binding protein Stemm cells Lc-ms/ms 
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