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ProteomicsProteome-wide identification of ISG15 sites in HeLa cells
We employed a GlyGly-peptidomics approach to comprehensively map ISG15 modification sites in HeLa cells under three conditions: mock-transfected controls, IFN-induced cells, and cells transfected with the ISGylation machinery. Lysates from wild-type and Isg15 knockout (KO) cells were processed using...
ORGANISM(S): Homo sapiens (Human) 
2026-03-02 | PXD065158 | Pride
Ptm Hela Glygly Isg15 Gg-peptidomics 
ProteomicsProteome-wide identification of ISG15 sites targeted by SARS-CoV-2 PLpro
Here, we applied an ISG15-GlyGly peptidomics approach on HeLa cells producing high levels of ISGylated proteins upon stimulation with IFN-α. After obtaining cellular lysates from wild-type and Isg15-/-cells, the samples were incubated with recombinant wild-type or mutant PLpro to catalyze deISGylati...
ORGANISM(S): Homo sapiens (Human) 
2021-10-12 | PXD026748 | Pride
Interferon Sars-cov-2 Isg15 
ProteomicsUSP24 is an ISG15 cross-reactive deubiquitinase that mediates IFN-I production by de-ISGylating the RNA helicase MOV10
The interferon Stimulated Gene-15 (ISG15) is a ubiquitin-like modifier induced by type I Interferon (IFN-I) and plays a crucial role in the innate immune response against viral infections. ISG15 is conjugated to target proteins by an enzymatic cascade through a process called ISGylation. While USP18...
ORGANISM(S): Homo sapiens (Human) 
2026-05-13 | PXD055843 | Pride
Human Innate immunity Ubiquitomics Isg15 Immunology Usp24 
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