Sort   by:  
 Page size 
ProteomicsExpanding the substantial interactome of NEMO using protein microarrays
Signal transduction by the NF-kappaB pathway is a key regulator of a host of cellular responses to extracellular and intracellular messages. The NEMO adaptor protein lies at the top of this pathway and serves as a molecular conduit, connecting signals transmitted from upstream sensors to the downstr...
ORGANISM(S): Homo sapiens 
2010-06-19 | E-GEOD-19841 | biostudies-arrayexpress
TranscriptomicsGenome-wide identification of BRAT- and PUM-associated mRNAs reveals PUM-independent functions for BRAT in translational repression and mRNA degradation during the Drosophila maternal-to-zygotic transition
Here, we analyze the RNA-binding preferences of the Drosophila melanogaster BRAT protein using RNAcompete. In the RNAcompete assay, purified GST-tagged BRAT is incubated with an excess of RNA pool and bound RNA from individual pulldown experiments are directly labeled and hybridized to a custom Agil...
ORGANISM(S): synthetic construct 
2015-03-30 | E-GEOD-60498 | biostudies-arrayexpress
TranscriptomicsThe crystal structure of the NHL domain in complex with RNA reveals the molecular basis of Drosophila Brain tumor-mediated gene regulation
Here, we analyze the RNA-binding preferences of several NHL containing RNA-binding proteins (BRAT, NCL-1, TRIM71, WECH, LIN-41, TRIM56, and MEI-P26) using RNAcompete. In the RNAcompete assay, purified GST-tagged RNA-binding protein is incubated with an excess of RNA pool and bound RNA from individua...
ORGANISM(S): synthetic construct 
2015-09-14 | E-GEOD-73000 | biostudies-arrayexpress
ProteomicsThe proteins interacting with GST-betaTub85D from the GST pulldown
First, GST-betaTub85D and GST proteins are expressed, followed by pulldown experiments with glutathione sepharose, and finally, mass spectrometry is used to identify the captured proteins.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2024-09-29 | PXD056093 | Pride
Betatub85d Wolbachia Drosophila 
ProteomicsPulldown GST-SH3 domains of Endophilin and rat brain lysates
Pulldown experiments using GST-SH3 domains from hman Endophilin A1, A2 and A3 and rat brain lysates
ORGANISM(S): Rattus norvegicus (Rat) 
2018-08-06 | PXD010090 | Pride
Pulldown Endophilin Rat brain lysates 
ProteomicsGST-OsHSFA4d pulldown of rice total proteins
For liquid GST pull-down tandem with LC-MS/MS analysis, the gels were cut and sent to Shanghai Bioprofile (Shanghai, China) for analysis.
ORGANISM(S): Oryza sativa (Rice) 
2025-06-09 | PXD064744 | Pride
Gst pulldown  oshsfa4d 
ProteomicsIdentification of SUN5 interactors from mouse testis lysate
GST pulldown assay was used to identify SUN5 interactors from testis lysate. Two bands were pulled down, subjected to LC-MS analysis.
ORGANISM(S): Mus musculus (Mouse) 
2018-01-25 | PXD007815 | Pride
Lc-ms/ms Gst pulldown Sun5 
ProteomicsPulldown of Maspin-GST in Human Cells Treated or Untreated with Nocodazole
Human cells, either asynchronous or synchronized with nocodazole, were subjected to a pulldown assay using GST-maspin. Interacting proteins were eluted using 10 mM reduced glutathione, 150 mM NaCl, 1 mM DTT, and 50 mM Tris-HCl (pH 8.5).
ORGANISM(S): Homo sapiens (Human) 
2025-09-01 | PXD057847 | Pride
Pulldown Human cells Nocodazole Maspin 
TranscriptomicsConserved functional antagonism between CELF and MBNL proteins regulates stem cell-specific alternative splicing and regeneration in planarians
Here, we analyze the RNA-binding preferences of the planarian smed-mbnl-like-2, smed-mbnl-1 (isoform X1), smed-bruli, smed-mbnl-1 (isoform Xins), and smed-mbnl-like-1 protein using RNAcompete. In the RNAcompete assay, a purified GST-tagged protein is incubated with an excess of RNA pool and bound RN...
ORGANISM(S): synthetic construct 
2016-08-01 | E-GEOD-75554 | biostudies-arrayexpress
ProteomicsIdentification of novel KRT32-associated proteins in primary keratinocytes cells lysate by GST-KRT32 pulldown assays coupled with mass spectrometry.
GST-KRT32 and its interacting proteins in primary keratinocytes cells were enriched by pull-down using glutathione-Sepharose beads. Then, KRT32-interacting proteins were identified by mass spectrum. Proteins obtained in Pulldown were subjected to SDS-PAGE gel electrophoresis and silver staining. The...
ORGANISM(S): Homo sapiens (Human) 
2024-06-17 | PXD050141 | Pride
Keratin  krt32 
Sort   by:  
 Page size