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ProteomicsReduced structural rigidity of MDMX protein enhances binding to TP53 mRNA
The two murine double minute (MDM) family members, MDM2 and MDMX are a well-established negative regulator of p53 activity. Under DNA damage conditions, MDM2 and MDMX are phosphorylated near their RING domains (serine 395 at MDM2 and serine 403 at MDMX) and switch to act as p53 positive regulators. ...
ORGANISM(S): Homo sapiens (Human) 
2026-01-05 | PXD060074 | Pride
Human Mdmx-mdm2 interaction P53 mrna stabilization Ring domain Hdx-ms mapping Lc-ms/ms 
ProteomicsThe sequence-specific peptide-binding activity of the protein sulfide isomerase AGR2 directs its’ stable binding to the oncogenic receptor EpCAM
AGR2 is an oncogenic endoplasmic reticulum (ER)-resident protein disulfide isomerase. AGR2 protein has a relatively unique property for a chaperone in that it can bind sequence-specifically to a peptide motif (TTIYY). A synthetic TTIYY-containing peptide column can be used to affinity-purify AGR2 f...
ORGANISM(S): Escherichia coli 
2018-01-22 | PXD005782 | Pride
Human Hdx Agr2 Protein-protein interaction Epcam Lc-ms/ms 
ProteomicsPathway Analysis of Dominant CHIP E3 Ligase-Dependent Protein Steady State Changes Highlight a Role in Neuronal Cell Membrane Integrity Linked to the Actin Cytoskeleton
Functional integrity of the E3-ubiquitin ligase CHIP is essential for healthy aging in the brain. By virtue of its association with the core molecular chaperone machinery, studies into the physiological role(s) of CHIP have been dominated by its involvement in the quality control and degradation of ...
ORGANISM(S): Homo sapiens (Human) 
2021-07-29 | PXD021404 | Pride
Human Neurons Proteomics Swath Mass spectrometry 
ProteomicsHydrogen Deuterium Exchange Mass Spectrometry implicates dynamic CD20 antigen binding to two discontinuous CDR regions in the NCD1.2 monoclonal antibody
A comparative canine-human therapeutics model is being developed in B-cell lymphoma through the generation of a hybridoma cell that produces a murine monoclonal antibody specific for canine CD20. The hybridoma cell produces two light chains, light chain-3 and light chain-7. However, the contribution...
ORGANISM(S): Canis lupus x Canis lupus familiaris 
2021-09-09 | PXD018510 | Pride
Human Comparative medicine Lymphoma Monoclonal antibody Hdx Adept Phage display Cd20 Lc-ms/ms 
ProteomicsHDX-MS implicates dynamic CD20 antigen binding to two discontinuous CDR regions in the NCD1.2 monoclonal antibody
A comparative canine-human therapeutics model is being developed in B-cell lymphoma through the generation of a hybridoma cell that produces a murine monoclonal antibody specific for canine CD20. The hybridoma cell produces two light chains, light chain-3 and light chain-7. However, the contribution...
ORGANISM(S): Canis lupus x Canis lupus familiaris 
2021-09-09 | PXD018550 | Pride
Human Comparative medicine Lymphoma Monoclonal antibody Hdx Adept Phage display Cd20 
ProteomicsTranslation of bi-directional transcripts generates peptides for the MHC-I pathway.
Antisense transcripts play an important role in generating regulatory non-coding RNAs but if these transcripts are also translated and for what purpose is yet poorly understood. Isolation of polysome fractions followed by RNA sequencing and mass spectrometry analysis of a six frame data-base reveale...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD057573 | Pride
Human Mass spectrometry Polysomes Mhc-i pathway 
ProteomicsHDX-MS of CHIP wt and K30A mutant
HDX-MS was used to establish that conformational-inhibition-signals extended from the TPR-domain to the U-box of CHIP. This underscores inter-domain allosteric regulation of CHIP by the core molecular chaperones. Defining the chaperone-associated TPR-domain of CHIP as a manager of inter-domain commu...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2015-07-30 | MSV000079205 | MassIVE
ProteomicsExcess protein synthesis, denatured proteins or molecular crowding spontaneously induce HSF1 trimerisation and activation
Stress responses are a key feature of normal physiology and are usurped by cancer cells to ensure enhanced protein synthesis for growth, to compensate for genomic instability, and to protect cancer cells from therapy induced stress. Heat shock factor-1 (HSF1) is a major stress-response transcription...
ORGANISM(S): Homo sapiens (Human) 
2024-08-14 | PXD037662 | Pride
Lc-msms Hsf1 Stress Hsp70 Hsp90 Crowding Hdx-ms Protein folding 
ProteomicsInherited anemia-associated mutations disrupt Codanin1 and CDIN1 mutual direct binding
Congenital dyserythropoietic anemia type I (CDA-I) is a rare hereditary disease that causes ineffective erythropoiesis and morphological abnormalities in bone marrow erythroblasts. The congenital abnormalities are manifested by internuclear bridges and Swiss-cheese-like heterochromatin. CDA-I is as...
ORGANISM(S): Homo sapiens (Human) 
2026-09-07 | PXD037661 | Pride
Rare diseases Cdin1 Lc-msms C15orf41 Codanin1 Congenital dyserythropoietic anemia Hdx-ms 
ProteomicsSRSF1 as a novel interacting partner for IFITM1/3 unravels the emergent role of IFITM1/3 mediating protein expression
IFITM proteins play a role in cancer cell progression through undefined mechanisms. Recent data demonstrated that interferon induced transmembrane 1 and 3 (IFITM1/3) proteins are required for the interferon gamma (IFNγ) stimulated synthesis of a subset of IFN-stimulated proteins; including human leu...
ORGANISM(S): Homo sapiens (Human) 
2022-10-13 | PXD030540 | Pride
Mass spectrometry Interferon Ribosome Isoform of serine and arginine rich splicing factor 1 Crispr/cas Mrna Interferon induced transmembrane 1 and 3 
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