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MetabolomicsRedirected nuclear glutamate dehydrogenase supplies Tet3 with α-ketoglutarate in neurons

Tet3 is the main α-ketoglutarate (αKG)-dependent dioxygenase in neurons that converts 5-methyl-dC into 5-hydroxymethyl-dC and further on to 5-formyl- and 5-carboxy-dC. Neurons possess high levels of 5-hydroxymethyl-dC that further increase during neural activity to establish transcriptional plast...

2021-06-24 | MTBLS2852 | MetaboLights
TranscriptomicsIdentification of the miRNA targetome in hippocampal neurons using RIP-seq
We established a neuron-specific Argonaute2:GFP-RNA immunoprecipitation followed by high throughput sequencing (AGO2-RIP-seq) to analyse the regulatory role of miRNAs in mouse hippocampal neurons. Using this technique, we identified more than two thousand miRNA target genes in hippocampal neurons, r...
ORGANISM(S): Mus musculus 
2015-07-31 | E-GEOD-65543 | biostudies-arrayexpress
TranscriptomicsStudy on miRNA expression pattern in Aging Mouse Hippocampal Neurons
Mouse hippocampal microRNA 3 samples
ORGANISM(S): Mus musculus 
2016-10-27 | E-GEOD-74796 | biostudies-arrayexpress
ProteomicsRat hippocampal neurons' proteomes
We induced over-expression and under-expression of Camk2b in cultured rat hippocampal neurons through transfection with lentivirus plasmids. Then isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomics followed by bioinformatics analyses were carried out to explore...
ORGANISM(S): Rattus Norvegicus 
2022-07-27 | PXD033931 |
ProteomicsPhactr1/PP1 phosphatase substrates in mouse primary neurons
Neurons express Phactr1 at high level (Allen et al., 2004), and Phactr1 mutations are associated with morphological and functional developmental defects in cortical neurons (Hamada et al., 2018). To identify targets of the Phactr1/PP1 phosphatase holoenzyme, we cultured hippocampal and cortical neur...
ORGANISM(S): Mus musculus (Mouse) 
2020-10-28 | PXD019882 | Pride
Neurons Phosphatase Mouse Phactr1 
TranscriptomicsThe histone lysine demethylase Kdm6b is required for activity-dependent preconditioning of hippocampal neuronal survival
We profiled basal and bicuculline+4-AP inducible mRNA expression in cultured mouse hippocampal neurons with or without viral shRNA mediated knockdown of Kdm6b We harvested mRNA from neurons under four conditions (pLKO vector/treatment control, pLKO vector/3hr bicuculline+4AP, Kdm6b knockdown/treatme...
ORGANISM(S): Mus musculus 
2014-06-12 | E-GEOD-58398 | biostudies-arrayexpress
TranscriptomicsHyper-excitability of Neurons generated from Patients with Bipolar Disorder
Bipolar Disorder (BD) is a complex neuropsychiatric disorder that is characterized by intermittent episodes of mania and depression and, without treatment, 15% of patients commit suicide1. Hence, among all diseases, BD has been ranked by the WHO as a top disorder of morbidity and lost productivity2....
ORGANISM(S): Homo sapiens 
2015-10-30 | E-GEOD-58933 | biostudies-arrayexpress
TranscriptomicsDCDC2 overexpression in rat primary hippocampal neurons
Transcriptome profiling of rat primary hippocampal neurons when the dyslexia candidate gene DCDC2 is overexpressed using transient transfections.

Additional processed data files and their associated custom CDF are available on the FTP site for this experiment.
ORGANISM(S): Rattus norvegicus 
2011-03-01 | E-MTAB-518 | biostudies-arrayexpress
ProteomicsPhosphoproteome of five-minute KCl-depolarised mouse cultured hippocampal neurons
Cultured hippocampal neurons from mice were treated with high concentration KCl for five minutes to depolarise the plasma membrane and model sustained brain activity. A high KCl condition was compared to low KCl using phosphoproteomics. Phosphopeptides were enriched from trypsin digested bio-replica...
ORGANISM(S): Mus musculus (Mouse) 
2023-10-15 | PXD020232 | Pride
Neurons Depolarisation Phosphoproteome Mouse Hippocampal Tau Kcl Lc-ms/ms 
TranscriptomicsMolecular Signatures of Neurogenesis in the Hippocampal Subgranular Zone of Rodents
We sought to find molecular signatures of the SGZ cell types, and to characterize the molecular pathways and transcription factor cascades that define the neurogenic niche. We used laser capture microdissection and DNA microarrays to profile gene expression in the inner (SGZ) and outer portions of t...
ORGANISM(S): Mus musculus 
2013-12-04 | E-GEOD-39697 | biostudies-arrayexpress
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