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ProteomicsA Parallel Accumulation-Mobility Aligned Fragmentation Strategy Utilizing High-Resolution Ion Mobility for High Performance Proteomics Analysis: Low-Load HeLa Data Sets for PAMAF and DDA Modes
Here we present a parallel accumulation-mobility aligned fragmentation (PAMAF) mode that expands the capabilities of the MOBILion Systems MOBIE® high-resolution ion mobility (HRIM) system for mass spectrometry-based proteomics. The MOBIE system leverages structures for lossless ion manipulation (SL...
ORGANISM(S): Homo sapiens (Human) 
2026-07-11 | PXD069951 | Pride
Human Pamaf Ion mobility Qtof Slim Hela Hrim Bottom-up proteomics 
UnknownKinesin-8B controls basal body function and flagellum formation and is key to malaria transmission.
Not available
S-EPMC6696982 | biostudies-literature
ProteomicsA Parallel Accumulation-Mobility Aligned Fragmentation Strategy Utilizing High-Resolution Ion Mobility for High Performance Proteomics Analysis: Bulk HeLa Data Sets for PAMAF, pDIA-PAMAF, and DDA Modes
Here we present a novel data independent acquisition mass spectrometry operating mode termed parallel accumulation-mobility aligned fragmentation (PAMAF) that offers enhanced speed and sensitivity for ion fragmentation analysis for nontargeted discovery workflows such as bottom-up proteomics. This m...
ORGANISM(S): Homo sapiens (Human) 
2026-07-11 | PXD069730 | Pride
Human Pamaf Ion mobility Qtof Slim Hela Hrim Bottom-up proteomics 
ProteomicsReal-time dynamics of Plasmodium Ndc80 reveals unusual modes of chromosome segregation during parasite proliferative stages
Eukaryotic cell proliferation requires chromosome replication and precise segregation to ensure daughter cells have identical genomic copies. The genus Plasmodium, the causative agent of malaria, has developed remarkable characteristic of nuclear division throughout its lifecycle to meet some peculi...
ORGANISM(S): Plasmodium berghei ANKA 
2020-06-09 | PXD017619 | Pride
Ndc80 Chromosome segregation Malaria Parasite Proliferation Warwick-rtp Plasmodium 
UnknownReal-time dynamics of <i>Plasmodium</i> NDC80 reveals unusual modes of chromosome segregation during parasite proliferation.
Not available
S-EPMC7340582 | biostudies-literature
ProteomicsFish Liver Immune Response LC-MSMS
High-throughput proteomics was used to determine the role of the fish liver in defense responses to bacterial infection, done using a rainbow trout (Oncorhynchus mykiss) model following infection with Aeromonas salmonicida, the causative agent of furunculosis. The vertebrate liver has multifaceted r...
ORGANISM(S): Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) 
2018-10-08 | PXD010186 | Pride
Lc-msms Rainbow trout Maxquant Fish Liver Immune 
UnknownPlasmodium kinesin-8X associates with mitotic spindles and is essential for oocyst development during parasite proliferation and transmission.
Not available
S-EPMC6786531 | biostudies-literature
ProteomicsPlasmodium condensin core subunits (SMC2/SMC4) mediate atypical mitosis and are essential for parasite proliferation and transmission
Condensin is a multi-subunit protein complex regulating chromosome condensation and segregation during cell division. In Plasmodium spp., the causative agent of malaria, cell division is atypical and the role of condensin is unclear. Here we examine the role of SMC2 and SMC4, the core subunits of co...
ORGANISM(S): Plasmodium berghei ANKA 
2020-05-27 | PXD016833 | Pride
Condensin Malaria Parasite Lc-ms Scm2 Warwick-rtp Plasmodium berghei Scm4 
TranscriptomicsExposure to ionizing radiation induced persistent gene expression changes in mouse mammary gland
Six to eight week old female C57BL/6J mice were exposed to 2 Gy of whole body γ radiation and mammary glands were surgically removed 2-month after radiation. RNA was isolated and microarray hybridization performed for gene expression analysis. 5 samples were analyzed: 2 controls at 2 months, 1 2 Gy ...
ORGANISM(S): Mus musculus 
2014-05-01 | E-GEOD-57196 | biostudies-arrayexpress
TranscriptomicsGenomeTiling
Samples were purchased from Clontech as mRNA. Hybridization material was generated through a random-priming amplification procedure (RP-AMP) using primers with a random sequence at the 3' end and a fixed motif at the 5' end. shDNP256 (first strand synthesis): 5'- TAG ATG CTG TTG NNN NNN NNN -3' sh...
ORGANISM(S): Homo sapiens 
2004-03-03 | E-GEOD-1097 | biostudies-arrayexpress
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