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ProteomicsRegulated local synthesis of ribosomal proteins maintains ribosome function in axons
Ribosome assembly occurs mainly in the nucleolus yet recent studies have revealed robust enrichment and translation of mRNAs encoding many ribosomal proteins (RPs) in axons, far away from neuronal cell bodies. Here, we report a physical and functional interaction between locally synthesized RPs and ...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2019-12-09 | PXD015574 | Pride
Ribosomes Sucrose density gradient fractionation Axon 
ProteomicsLC-MSMS of DCC and Neuropilin-1 immunoprecipitated samples from Xenopus Laevis brains
During neuronal wiring, extrinsic cues trigger the local translation of specific mRNAs in axons via cell surface receptors. The coupling of ribosomes to receptors has been proposed as a mechanism linking signals to local translation but it is not known how broadly this mechanism operates, nor whethe...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2019-12-02 | PXD015650 | Pride
Lc-msms Neuropilin-1 Dcc Xenopus laevis brains Lfq Immunoprecipitation 
ProteomicsSILAC-labeled proteins in ribosome samples
we developed a novel method combining axon-pSILAC17 with axonal ribosome purification. In this method, we first labeled newly synthesized proteins with heavy amino acids (AAs) in somaless axons cultured in a Boyden chamber. Approximately 2000 eyes were cultured for each experiment to obtain sufficie...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2019-12-09 | PXD011032 | Pride
Xenopus laevis Axon Ribosome Silac 
TranscriptomicsmiR-124 acts through coREST to control the onset of Sema3A sensitivity in navigating retinal growth cones
During axon pathfinding, growth cones commonly exhibit changes in sensitivity to guidance cues that follow a strict timetable, even in the absence of pathway feedback, implicating cell-intrinsic regulation. Cellular timer mechanisms, however, are poorly understood. Here we have investigated microRNA...
ORGANISM(S): Xenopus laevis 
2011-12-12 | E-GEOD-33444 | biostudies-arrayexpress
TranscriptomicsSubcellular expression profiling of the growth cones of retinal ganglion cells (RGC)
Cue-directed axon guidance depends partly on local translation in growth cones. Many mRNA transcripts are known to reside in developing axons yet little is known about their subcellular distribution or, specifically, which transcripts are in growth cones. Laser capture microdissection (LCM) was used...
ORGANISM(S): Xenopus laevis 
2010-11-23 | E-GEOD-25166 | biostudies-arrayexpress
TranscriptomicsThe dynamic translatome of retinal ganglion cell axons during assembly and maintenance of the mouse visual system
Local mRNA translation mediates the adaptive responses of axons to extrinsic signals but direct evidence that it occurs in mammalian CNS axons in vivo is scant. We developed an axon-TRAP-RiboTag approach in mouse that allows deep-sequencing analysis of ribosome-bound mRNAs in the retinal ganglion ce...
ORGANISM(S): Mus musculus 
2016-06-16 | E-GEOD-79352 | biostudies-arrayexpress
TranscriptomicsGlobal transcriptome analysis reveals differences between embryonic and adult dorsal root ganglion axonal mRNAs that are implicated in axonal growth and pain
Local protein synthesis in sensory neuron axons is necessary for axonal regeneration with the efficiency of regeneration decreasing with age. Because the full repertoire of transcripts in embryonic and adult rat sensory axons is unknown we asked how the pool of mRNAs dynamically changes during age...
ORGANISM(S): Rattus norvegicus 
2010-11-23 | E-GEOD-22638 | biostudies-arrayexpress
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