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ProteomicsHPLC-MS datasets of LINP1 RNA pulldown samples in A431 cells
We performed LINP1 RNA pulldown followed by HPLC-MS analysis to identify LINP1-interacting proteins in A431 cells. With high performance liquid chromatography-mass spectrometry (HPLC-MS), the binding protein of LINP1 from RNA pull down was detected by removing non-specific protein which were binding...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD032027 | Pride
Human a431 cells hplc-ms 
ProteomicsSac1 degron A431 cells 3h DMSO vs 3h IAA
Sac1 is a conserved phosphoinositide phosphatase, whose loss-of-function compromises cell and organism viability. Here, we employed acute auxin-inducible Sac1 degradation to identify its immediate downstream effectors in human cells. Most of Sac1 was is degraded in ~1 h, paralleled by increased PI(4...
ORGANISM(S): Homo sapiens (Human) 
2025-07-08 | PXD065794 | Pride
Human a431 cells Sac1 Auxin inducible degradation 
ProteomicsProbing tyrosine kinase inhibitors specificity
Using a three-pronged MS-based proteomic approach, we assessed the mode-of-action of four clinically-relevant tyrosine kinase inhibitors in an epithelial cancer cell line. Here we defined the: (I) semi-quantitative tyrosine kinome; (II) drug interactome; and (III) drug-induced effects on the tyrosin...
ORGANISM(S): Homo sapiens (Human) 
2014-05-12 | PXD000681 | Pride
Human Tyrosine kinase inhibitors Chemical proteomics Phosphoproteomics Phosphotyrosine Lc-ms/ms 
UnknownStudy of the betulin enriched birch bark extracts effects on human carcinoma cells and ear inflammation.
Not available
S-EPMC3527166 | biostudies-literature
UnknownSynthesis and biological evaluation of novel benzothiazole derivatives as potential anticancer and antiinflammatory agents.
Not available
S-EPMC10982501 | biostudies-literature
ProteomicsProximity biotinylation of A431 cells with gefitinib alone treatment by EGFR-FabID
A431cells were pretreated with DMSO or gefitinib. Then AGIA-FabID or EGFR-FabID were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by...
ORGANISM(S): Homo Sapiens (human) 
2023-11-14 | PXD043526 | JPOST Repository
UnknownPhytochemical investigations and antiproliferative secondary metabolites from Thymus alternans growing in Slovakia.
Not available
S-EPMC6130594 | biostudies-literature
ProteomicsIon mobility tandem mass spectrometry enhances performance of bottom-up proteomics
We describe a novel method utilizing ion mobility-based concentration of peptide fragment ions on a qTOF mass spectrometer improving the sensitivity of bottom-up proteomics by up to 10-fold. This enabled the identification of 7,500 human proteins within one day and 8,600 phosphorylation sites within...
ORGANISM(S): Homo sapiens (Human) 
2015-08-18 | PXD000863 | Pride
Quadrupole time of flight mass spectrometer Proteomics Ion mobility mass spectrometry Chemical proteomics Phosphoproteomics 
ProteomicsThe formation of the 'footprint of death' as a mechanism for generating large substrate-bound extracellular vesicles that mark the site of cell death
Apoptotic cells communicate to phagocytic cells through releasing soluble factors and apoptotic cell derived extracellular vesicles. However, whether there are additional factors that remain attached at the site of cell death to signal to phagocytic cells is currently unknown. Here we show that apop...
ORGANISM(S): Homo sapiens (Human) 
2025-10-15 | PXD067718 | Pride
Ev Extracellular vesicle The footprint of death F-apoev Food 
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