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ProteomicsApplication of VHH-immobilized cryogel-based immunoaffinity chromatography for isolation of extracellular vesicles from human plasma
Proteomics profiles of extracellular vesicles isolated using a new cryogel-based immunoaffinity chromatography system functionalized with single-domain VHH antibodies for selective isolation of EVs from human plasma.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2025-12-08 | PXD070239 | Pride
Lc-msms Immunoaffinity chromatography Nkuaprot002 Ubfc-evlab Extracellular vesicles Plasma Homo sapiens Nanobodies Expand-ev Timstof flex Nanoelute2 
ProteomicsCD9-HPLC-IAC Isolation of Serum Extracellular Vesicles for Proteomic Analysis
We developed a column-based CD9 antibody-immobilized HPLC immunoaffinity (CD9-HPLC-IAC) technology for EV isolation from a microliter-scale of serum for downstream proteomic analysis. The CD9-HPLC-IAC method achieved EV isolation from 40 μL of serum in 30 min with a yield of 8.0×109 EVs. Proteomic a...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD027025 | Pride
Serum Immunoaffinity isolation Cd9 Extracellular vesicles Hplc Proteomics 
UnknownIntegration of Lateral Filter Arrays with Immunoaffinity for Circulating-Tumor-Cell Isolation.
Not available
S-EPMC6534423 | biostudies-literature
UnknownHigh-throughput surface epitope immunoaffinity isolation of extracellular vesicles and downstream analysis.
Not available
S-EPMC11272960 | biostudies-literature
UnknownColumn-based Technology for CD9-HPLC Immunoaffinity Isolation of Serum Extracellular Vesicles.
Not available
S-EPMC8496948 | biostudies-literature
UnknownApplication of VHH-Immobilized Cryogel-Based Immunoaffinity Chromatography for Isolation of Extracellular Vesicles.
Not available
S-EPMC12654627 | biostudies-literature
ProteomicsAdaptation of an immunoaffinity chromatography protocol for the isolation of the cell line immunopeptides
BACKGROUND. For developing targeted immunotherapy against ovarian adenocarcinoma, direct identification of peptides presented by major histocompatibility complex class I (HLA I) on the surface of tumor cells can be utilized. However, standard immunopeptidomics protocols may vary substantially in the...
ORGANISM(S): Homo sapiens (Human) 
2026-04-15 | PXD077080 | Pride
UnknownImmunoaffinity-based isolation of melanoma cell-derived exosomes from plasma of patients with melanoma.
Not available
S-EPMC5827723 | biostudies-literature
ProteomicsIdentification and Quantification of Human Relaxin Proteins by Immunoaffinity-Mass Spectrometry
We developed immunoaffinity-selected reaction monitoring assays for the measurment of human relaxin-1 and relaxin-2 proteins in biological and clinical samples.
ORGANISM(S): Homo sapiens (Human) 
2024-07-04 | PXD051230 | Pride
Relaxin h2 Relaxin h1 Rel1_human Mass spectrometry Rln2 Prostate Rln1 Immunoaffinity Rel2_human Maternal serum Enzyme-linked immunosorbent assay 
ProteomicsAdaptation of immunoaffinity protocol for the isolation of the cell line immunopeptides - immunopeptidomics part
BACKGROUND: The peptide repertoire presented by Human Leukocyte Antigens (HLA) provides a snapshot of intracellular proteins, which is monitored by the immune system. Although mass spectrometry is the gold standard for identification of these peptides, the upstream steps of the immunopeptidomics wor...
ORGANISM(S): Homo sapiens (Human) 
2026-04-15 | PXD077041 | Pride
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