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ProteomicsIdentification and Quantification of Human Relaxin Proteins by Immunoaffinity-Mass Spectrometry
We developed immunoaffinity-selected reaction monitoring assays for the measurment of human relaxin-1 and relaxin-2 proteins in biological and clinical samples.
ORGANISM(S): Homo sapiens (Human) 
2024-07-04 | PXD051230 | Pride
Relaxin h2 Relaxin h1 Rel1_human Mass spectrometry Rln2 Prostate Rln1 Immunoaffinity Rel2_human Maternal serum Enzyme-linked immunosorbent assay 
ProteomicsMET-dependent modulation of cellular responses to ionizing radiation by quantitative phosphoproteomics
Increasing evidence suggests that inhibiting growth factor receptor tyrosine kinases (RTKs) signaling may modulate cellular responses to DNA-damaging agents widely used in cancer treatment. In that respect, the MET RTK is deregulated in abundance and/or activity in a variety of human tumors. Using t...
ORGANISM(S): Homo sapiens (Human) 
2020-05-15 | PXD009433 | Pride
Phosphoproteomics Immunoaffinity Selected reaction monitoring (srm) Targeted proteomics 
ProteomicsApplication of VHH-immobilized cryogel-based immunoaffinity chromatography for isolation of extracellular vesicles from human plasma
Proteomics profiles of extracellular vesicles isolated using a new cryogel-based immunoaffinity chromatography system functionalized with single-domain VHH antibodies for selective isolation of EVs from human plasma.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2025-12-08 | PXD070239 | Pride
Lc-msms Immunoaffinity chromatography Nkuaprot002 Ubfc-evlab Extracellular vesicles Plasma Homo sapiens Nanobodies Expand-ev Timstof flex Nanoelute2 
ProteomicsMouse Stereocilia Capping Protein Immunoaffinity Purification
Capping protein controls stereocilia length and width during hair bundle development. To determine what other proteins are involved in capping protein regulation, we carried out immunoaffinity purifications targeted at either CAPZA or CAPZB2. The starting material for immunopurification was crude st...
ORGANISM(S): Mus musculus (Mouse) 
2017-08-25 | PXD006077 | Pride
Auditory Balance Vestibular Hair cells Stereocilia Hearing Actin Twinfilin Capz Hair bundle 
UnknownImmunoaffinity-enriched salivary small extracellular vesicles in periodontitis.
Not available
S-EPMC11648426 | biostudies-literature
UnknownA robust multiplex immunoaffinity mass spectrometry assay (PromarkerD) for clinical prediction of diabetic kidney disease.
Not available
S-EPMC7576806 | biostudies-literature
UnknownPeptide level immunoaffinity enrichment enhances ubiquitination site identification on individual proteins.
Not available
S-EPMC3879610 | biostudies-literature
OtherHuman Argonaute Immunoaffinity Purification-associated RNA
Immunoaffinity Purifications of Human Argonaute Twelve hours after transfections with mock or with miR-124, we washed each 15-cm plate once with phosphate-buffered saline (usually two plates were used per IP), then added 1 ml of 4 C lysis buffer (150 mM KCl, 25 mM Tris-HCl [pH 7.4], 5 mM Na-EDTA [pH...
ORGANISM(S): Homo sapiens 
2009-11-10 | GSE18834 | GEO
ProteomicsMyosin VIIA 138-1 immunoaffinity purification
Through its activity in stereocilia, MYO7A (myosin VIIA) is essential for hair cell function in the inner ear. Utilizing multiple stages of immunoaffinity enrichment, we have developed a strategy that allows us to partially purify stereocilia membranes from thousands of chick inner ears and isolate ...
ORGANISM(S): Gallus gallus (Chicken) 
2016-08-16 | PXD004221 | Pride
Auditory Myosin Vestibular Stereocilia Hair cell Pdz Hair bundle 
UnknownIncorporation of lateral microfiltration with immunoaffinity for enhancing the capture efficiency of rare cells.
Not available
S-EPMC7450051 | biostudies-literature
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