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ProteomicsIn vivo identification of GTPase interactors by mitochondrial relocalizationand proximity biotinylation
The ~160 small GTPases that comprise the Ras superfamily include many major regulators of growth, membrane traffic and the cytoskeleton, and a wide range of diseases are caused by mutations in particular members. They function as switchable landmarks with the active GTP-bound form recruiting to the ...
ORGANISM(S): Homo sapiens (Human) 
2019-07-17 | PXD013668 | Pride
Human Mitochondria gtpase Bira* Ras Lc-ms/ms Membranes Gefs 
ProteomicsBIOSITe: A novel method for direct detection of site specific biotinylation
Proximity-dependent biotinylation methods, such as those mediated by BioID and APEX, are being widely adopted for studying in vivo protein-protein interaction dynamics and subcellular structures. However, these along with other biotin-based technologies are severely dependent on avidin family protei...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2017-12-19 | PXD007862 | Pride
Bioid Proximity dependent biotinylation Bcr abl Mass spectrometry Ip Peptide Biotinylation Biosite 
ProteomicsA genetic platform to delineate tissue-specific in vivo secretion
At present, it remains difficult to deconvolute serum in order to identify the cell or tissue origin of a given circulating protein. Here, by exploiting the properties of proximity biotinylation, we describe a mouse model that enables the elucidation of the in vivo tissue-specific secretome. As an e...
ORGANISM(S): Mus musculus (Mouse) 
2021-01-14 | PXD022694 | Pride
Er-bioidha Endothelial cells Serum Secretome Biotinylation Muscle 
ProteomicsRapid proximity biotinylation of the Plasmodium falciparum exported protein, SBP1
The deadly human malaria-causing parasite, Plasmodium falciparum relies on its capacity to completely remodel its host red blood cell (RBC) through the export of several hundred parasite proteins across several membranes to the RBC.We fused the exported membrane protein, skeleton binding protein 1 (...
ORGANISM(S): Plasmodium Falciparum Nf54 (ncbitaxon:5843) 
2022-06-27 | MSV000089743 | MassIVE
UnknownChromatin Affinity Purification (ChAP) from Arabidopsis thaliana Rosette Leaves Using in vivo Biotinylation System.
Not available
S-EPMC8203944 | biostudies-literature
ProteomicsProteomic analysis of Mycobacterial Biofilm Matrix and Development of Biofilm-binding Synthetic Nanobodies
Tissue-penetrating nanobodies as drug-delivery vehicles have shown efficacy in treating hard-to-reach and -treat diseases. The present study reports nanobodies with application potential in the treatment of tuberculosis (TB), especially the antibiotic tolerant biofilms that form in TB granulomas. Us...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) Mycobacterium marinum 
2023-03-30 | PXD033425 | Pride
Synthetic nanobody Mycobacterium Proteomics Sybody Surface proteome Biofilm Biotinylation 
ProteomicsOff the shelf proximity biotinylation using ProtA-TurboID
Proximity biotinylation is a commonly used method to identify the in vivo proximal proteome for proteins of interest. This technology typically relies on fusing a bait protein to a biotin ligase using overexpression or CRISPR-based tagging, thus prohibiting such assays in (primary) cell types that a...
ORGANISM(S): Homo sapiens (Human) 
2022-10-17 | PXD030963 | Pride
Cell lines Centromeres Biotinylation Histone ptms Protein-protein interactions 
UnknownRegulatable in vivo biotinylation expression system in mouse embryonic stem cells.
Not available
S-EPMC3646753 | biostudies-literature
UnknownNovel system for in vivo biotinylation and its application to crab antimicrobial protein scygonadin.
Not available
S-EPMC4447092 | biostudies-literature
ProteomicsDevelopment of a method for quantitative analysis of in vivo interactions of pluripotency transcription factors Sox2, Oct4, Nanog by mass spectrometry
Protein-protein proximity of core pluripotency transcription factors plays an important role during cell reprogramming. Pluripotent embryonic stem (ES) cell identity is controlled by a trio of transcription factors: Sox2, Oct4, and Nanog. These proteins often bind to closely localized genomic sites....
ORGANISM(S): Homo sapiens (Human) 
2020-01-21 | PXD015756 | Pride
Mass spectrometry Oct4 Proximity utilizing biotinylation Transcription factors Sox2 Pluripotency 
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