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ProteomicsWhole cell proteomics analysis of Trypanosoma brucei 75kDa invariant surface glycoprotein knockout cells
A superfamily of invariant surface glycoproteins (ISGs) populates the African trypanosome surface, one of which, ISG75, is implicated in uptake of the century-old drug suramin. We disrupted the ISG75 gene locus encoding an array of six ISG75 paralogs on chromosome V by CRISPR/Cas9 knockout. Here we ...
ORGANISM(S): Trypanosoma brucei 
2023-03-11 | PXD031887 | Pride
Xenobiotics Drug metabolism Drug accumulation Trypanosome Crispr/cas9 Invariant surface glycoprotein Suramin 
ProteomicsPulldown of human serum proteins with Trypanosoma brucei Invariant Surface Glycoprotein 75
Subspecies of Trypanosoma brucei are the causative agents of Human African Trypanosomiasis (HAT) in Sub-Saharan Africa. The surface proteome of trypanosome parasites serves a critical function at the host-parasite interface and is essential for immune evasion. Most of the surface area is covered by ...
ORGANISM(S): Homo sapiens (Human) 
2024-02-06 | PXD047614 | Pride
Trypanosome Sleeping sickness Isg75 
UnknownInvariant surface glycoprotein 65 of Trypanosoma brucei is a complement C3 receptor.
Not available
S-EPMC9424271 | biostudies-literature
ProteomicsThe Trypanosoma brucei MISP family of invariant proteins are co-expressed with BARP as triple helical bundle structures on the surface of salivary gland forms, but is dispensable for parasite development within the tsetse vector
Trypanosoma brucei spp. develop into mammalian-infectious metacyclic trypomastigotes inside tsetse salivary glands. Besides acquiring a variant surface glycoprotein (VSG) coat, little is known about the metacyclic expression of invariant surface antigens. Proteomics analyses of saliva from T. brucei...
ORGANISM(S): Trypanosoma brucei 
2023-05-10 | PXD039684 | Pride
Proteomics Mass spectrometry Crystal structure Metacyclic Trypanosoma brucei Tsetse Misp Gpi 
ProteomicsTrypanosoma brucei SURFEOME: a validated surface proteome of the host-parasite interface
Surface membrane organization and composition is key to cellular function, and membrane proteins serve many essential roles in endocytosis, secretion and cell recognition. The surface of parasitic organisms, however, is a double-edged sword; this is the primary interface between parasites and their ...
ORGANISM(S): Trypanosoma brucei 
2015-06-15 | PXD002180 | Pride
Vsg Esag Surfeome Trypanosoma brucei Plasma membrane Cell surface proteome 
UnknownThe role of invariant surface glycoprotein 75 in xenobiotic acquisition by African trypanosomes.
Not available
S-EPMC9896412 | biostudies-literature
TranscriptomicsMaturation of myeloid dendritic cells through cognate interaction with invariant NKT cells
Aim of the experiment was to characterize the gene activation profile

induced within immature myeloid DCs by cell-cell contact with activated

invariant NKT cells under steady-state conditions.
ORGANISM(S): Mus musculus 
2010-10-08 | E-MEXP-2938 | biostudies-arrayexpress
ProteomicsThe endosomal TbTpr86/TbUsp7/SkpZ (TUS) complex controls surface protein abundance in trypanosomes
In trypanosomes two deubiquitilating enzymes (DUBs), orthologous to human USP7 and VDU1, control abundance of a cohort of surface proteins, including invariant surface glycoproteins (ISGs). Silencing TbUsp7 partially inhibits endocytosis and invariant surface glycoprotein turnover. S-phase kinase-as...
ORGANISM(S): Trypanosoma brucei 
2025-01-08 | PXD021000 | Pride
Ubiquitylation Skp1 Trypanosoma brucei Surface proteome Tus complex Cullin e3 ligase Usp7 
TranscriptomicsNKT-10 cells represent a novel invariant NKT cell subset with regulatory characteristics
We compared splenic Va14i NKT cells from C57BL/6 control mice and from mice injected 4 weeks earlier intravenously with 4ug/mouse of the iNKT cell antigen alpha-galactosylceramide (aGalCer). These mice were either left unstimulated or were stimulated with 1ug/mouse aGalCer i.v.. All mice were female...
ORGANISM(S): Mus musculus 
2014-07-25 | E-GEOD-47959 | biostudies-arrayexpress
UnknownUbiquitylation and developmental regulation of invariant surface protein expression in trypanosomes.
Not available
S-EPMC3147413 | biostudies-literature
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