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ProteomicsProteome profile of peripheral myelin in healthy mice and in a neuropathy model
Using the sciatic nerve as a model, we biochemically purified peripheral nervous system (PNS) myelin and systematically assessed its proteome by dedicated, partly ion mobility-enhanced data-independent acquisition (DIA) workflows with alternating low and elevated energy (MSE) modes. Specifically, we...
ORGANISM(S): Mus musculus (Mouse) 
2020-02-20 | PXD015960 | Pride
Transcriptome Axon degeneration Mouse Dynamic range enhancement Peripheral nervous system (pns) Charcot-marie-tooth disease Label-free quantification Myelin proteome Neuropathy Demyelination 
ProteomicsDifferential metabolic adaptations of brain cells to feeding a ketogenic diet in mice
It is largely unknown how cells in the central nervous system (CNS) adapt their energy metabolism to nutritional challenges or disease. To unravel the molecular basis of these adaptations, we generated a comprehensive proteome dataset of all major CNS cell types. Magnetic bead sorting was used to ac...
ORGANISM(S): Mus musculus (Mouse) 
2022-08-20 | PXD031376 | Pride
Inflammatory demyelinating disease Metabolomics Brain metabolism Label-free proteomics Mass spectrometry Ketogenic diet Transcriptomics 
ProteomicsSUMO1-conjugation is altered during normal aging but not by increased amyloid burden
SUMOylation is a post-translational protein modification that consists of the attachment of a SUMO (small ubiquitin-related modifier) moiety to a lysine residue of a target protein. Alteration in protein SUMOylation has been linked to the amyloid pathology apparent in Alzheimer’s disease. In the pre...
ORGANISM(S): Mus musculus (Mouse) 
2018-04-20 | PXD009166 | Pride
Brain Affinity purification Post-translational modification Alzheimer Mouse Small ubiquitin-related modifier (sumo) Label-free quantification Aging 
ProteomicsThe CNS myelin proteome: Deep profile and persistence after post-mortem delay
We revisited the proteome of myelin biochemically purified from the brains of healthy c56Bl/6N-mice utilizing complementary proteomic approaches for deep qualitative and quantitative coverage. A data-independent acquisition (DIA) workflow with alternating low and elevated energy (MSE) provided the h...
ORGANISM(S): Mus musculus (Mouse) 
2020-07-16 | PXD020007 | Pride
Mass spectrometry Oligodendrocyte Proteolipid protein (plp) Central nervous system (cns) Label-free proteomics Myelin basic protein (mbp) Post mortem-delay Cyclic nucleotide phosphodiesterase (cnp) Myelin proteome Transcriptome Demyelination Autopsy 
ProteomicsProteome profile of myelin in the zebrafish brain
While the myelin proteome is well characterized in mice, much less is known about the protein composition of myelin in non-mammalian species such as zebrafish (Danio rerio), despite its increasing popularity as model organism for myelin biology. Here, we assess the proteome of myelin biochemically p...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2021-03-22 | PXD023037 | Pride
Transcriptome Oligodendrocyte Mbp Label-free proteomics Teleost Cd59 Central nervous system (cns) Mpz Danio rerio Plp Zebrafish Myelin proteome 
ProteomicsA brain-enriched circular RNA controls excitatory neurotransmission and restricts sensitivity to aversive stimuli
The biological significance of circular RNAs remains largely unexplored due to the lack of loss-of-function animal models. In this study, we focused on circTulp4, a highly abundant circRNA that is enriched in the brain and synaptic compartments. We created a circTulp4-deficient (CD) mouse model and ...
ORGANISM(S): Mus musculus (Mouse) 
2024-04-15 | PXD045787 | Pride
Synaptosomes Label-free proteomics Mass spectrometry Circtulp4 Phenotyping Neurophysiology Circular rna 
ProteomicsHippocampal proteome profiling of a mouse model with dysfunctional STAT1 signaling
Signal transduction via the Signal Transducer and Activator of Transcription 1 (STAT1) pathway is indispensable for mediating the intracellular effects of interferon-α (IFN-α), interferon-γ (IFN-γ) and other cytokines in the brain and, thereby, crucial for antiviral and antibacterial responses durin...
ORGANISM(S): Mus musculus (Mouse) 
2025-07-14 | PXD062965 | Pride
Label-free protein quantification Interferon signaling Hippocampus Mass spectrometry Proteome analysis Transcriptome analysis Stat signaling 
ProteomicsA mouse photoreceptor proteome resource
To enrich mouse rod and cone photoreceptors for mass spectrometric analysis, we optimized our previously established trituration-based fluorescence-activated cell sorting (FACS) strategy (see Lux et al., 2024 for details; PMID: 38481472). For quantitative mass spectrometry, we utilized an ion mobili...
ORGANISM(S): Mus musculus (Mouse) 
2025-12-08 | PXD066755 | Pride
Label-free protein quantification Mass spectrometry Facs Retina Rod photoreceptors Cone photoreceptors Mpp6/pals2 
ProteomicsConservation and divergence of myelin proteome profiles between humans and mice
While myelin is commonly assessed in mice as a model for humans, it remained unclear to which extent their myelin protein composition is similar. We analyzed the proteome of myelin biochemically purified from human white matter by two steps of discontinuous sucrose density gradient centrifugation in...
ORGANISM(S): Homo sapiens (Human) 
2022-05-16 | PXD029727 | Pride
Oligodendrocyte Human post mortem brain tissue Label-free proteomics Mass spectrometry Central nervous system (cns) Autopsy Myelin proteome Translation 
ProteomicsProgressive axonopathy when oligodendrocytes lack the myelin protein CMTM5
Chemokine-like factor-like MARVEL-transmembrane domain containing protein 5 (CMTM5) is a tetraspan-transmembrane protein highly enriched in oligodendrocytes and central nervous system (CNS) myelin. We analyzed the proteome of myelin biochemically purified from mouse brains upon genetic disruption of...
ORGANISM(S): Mus musculus (Mouse) 
2022-04-04 | PXD029443 | Pride
Chemokine-like factor-like marvel-transmembrane domain containing protein (cmtm) family Oligodendrocyte Label-free proteomics Mass spectrometry Central nervous system (cns) Wallerian degeneration Neurodegeneration Myelin proteome 
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