Sort   by:  
 Page size 
ProteomicsLFQ proteomics analysis of M. smegmatis treated with BacPROTACs.
To monitor selective degradation of ClpC1 and ClpC2 using BacPROTAC compounds in M. smegmatis we performed a LFQ proteomics analysis treating the cells at bactericidal compound concentration. We detected degradation of both proteins and additional dysregulation of the mycobacterial PQC system.
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
2023-06-07 | PXD037235 | Pride
Antibiotics Tpd Bacprotac Protac Protein degradation 
ProteomicsLFQ proteomics analysis of the M. smegmatis ClpC2 interactome.
To assess the ClpC2 interactome, a IP-MS analysis was performed using ectopically expressed tagged ClpC2 in a ClpC2 deficient cell line. To evaluate potential binding sites on ClpC2, CymA treatment was applied to compete with potential interactors for a shared ClpC2 binding site.
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
2023-06-07 | PXD037231 | Pride
Antibiotics Tpd Protein degradation 
ProteomicsA quantitative proteomics approach to monitor targeted protein degradation in M. smegmatis
Targeted protein degradation via the ubiquitin proteasome has been established in eukaryotes, however no application has been shown in bacteria so far. In the present work, selective protein degradation is shown using hetero bifunctional drugs, bringing substrates in close proximity to the bacterial...
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Bacteria 
2022-08-12 | PXD021505 | Pride
Mycobacteria Targeted protein degradation Protease Tpd 
ProteomicsLFQ proteomics analysis of M. smegmatis treated with CymA and ecumicin (Part 1).
To assess the impact of the bactericidal compounds CymA and ecumicin on the mycobacterial proteome and to monitor proteome remodeling by the AAA+ ClpC1 protease machinery, we performed a LFQ proteomics analysis treating the cells at sub-bactericidal compound concentration. We identified new players ...
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
2023-06-07 | PXD037232 | Pride
Tuberculosis Tpd Protein degradation 
ProteomicsLFQ proteomics analysis of M. smegmatis treated with CymA and ecumicin (Part 2).
To assess the impact of the bactericidal compounds CymA and ecumicin on the mycobacterial proteome and to monitor proteome remodeling by the AAA+ ClpC1 protease machinery, we performed a LFQ proteomics analysis treating the cells at bactericidal compound concentration. We identified new players of t...
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
2023-06-07 | PXD037234 | Pride
Antibiotics Tuberculosis Tpd Protein degradation 
UnknownDual roles of mTORC1-dependent activation of the ubiquitin-proteasome system in muscle proteostasis.
Not available
S-EPMC9613904 | biostudies-literature
Transcriptomics3' mRNA-seq of human Ma-Mel-47 (melanoma) and Panc89 (PDAC) cells treated with the USP7 degraders NK266 and NK250, respectively, for 72 h.
3' mRNA-seq was used to study the effects of USP7 degradation by the degraders NK266 and NK250 on gene expression in a human melanoma and PDAC cell line, respectively.
ORGANISM(S): Homo sapiens 
2026-03-27 | E-MTAB-16302 | biostudies-arrayexpress
UnknownThe neuromuscular junction is a focal point of mTORC1 signaling in sarcopenia.
Not available
S-EPMC7481251 | biostudies-literature
ProteomicsUnexpected role of a truncated ribosome-associated chloroplast trigger factor homolog serving ribosome biogenesis in plants - Data set 2: Analysis of sedimented ribosomes
Molecular chaperones are essential throughout a protein's life and act already during protein synthesis. Bacteria and chloroplasts of plant cells share the ribosome-associated chaperone trigger factor (Tig1 in plastids), facilitating maturation of emerging nascent polypeptides. While typical trigger...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-12-09 | PXD057264 | Pride
Ribosomes Chaperones Arabidopsis 
ProteomicsUnexpected role of a truncated ribosome-associated chloroplast trigger factor homolog serving ribosome biogenesis in plants - Data set 1 (Global proteome of wt and tig2 mutants)
Molecular chaperones are essential throughout a protein's life and act already during protein synthesis. Bacteria and chloroplasts of plant cells share the ribosome-associated chaperone trigger factor (Tig1 in plastids), facilitating maturation of emerging nascent polypeptides. While typical trigger...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-12-09 | PXD057252 | Pride
Chaperone Arabidopsis 
Sort   by:  
 Page size