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ProteomicsIn vivo proximity labeling identifies the interactome of the endoribonuclease Zucchini in Drosophila ovary.
PIWI-interacting RNAs (piRNAs) are small RNAs that play a conserved role in genome defense by silencing transposable elements. The piRNA processing pathway is dependent on the sequestration of RNA precursors and protein factors in specific subcellular compartments. Therefore, a highly resolved spati...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-02-08 | PXD036182 | Pride
Turboid Drosophila Proximity labeling Lc-ms/ms 
ProteomicsDynamic tracking and identification of tissue-specific secretory proteins in the circulation of live mice
Accurate analysis of tissue-specific secretory proteins in blood plasma can provide valuable information regarding therapeutic target development but current approaches are mostly limited to ex vivo proteomic analysis. Here, we developed an in situ secretory protein labeling technology using endopla...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2021-08-06 | PXD025141 | Pride
Proteomics Proximity labeling Turboid 
ProteomicsCellular dsRNA interactome captured by K1 antibody reveals the regulatory map of exogenous RNA sensing
We developed an anti-dsRNA antibody co-immunoprecipitation coupled with quantitative mass spectrometry to comprehensively identify RBPs bound to cellular dsRNAs. To minimize false positives, we conducted a quantitative mass spectrometry analysis under two additional conditions: first, by treating th...
ORGANISM(S): Homo sapiens (Human) 
2025-02-03 | PXD053100 | Pride
Dsrna Dsrbp Interferon response Viral infection Mass spectrometry Rna sensing Crispr-cas9 screening Lc-ms/ms 
ProteomicsTissue-specific in vivo mitochondrial matrix proteome profiling in mouse
Targeting proximity labeling enzymes to specific cellular locations is a viable strategy for profiling subcellular proteomes with minimal perturbance to normal physiology. Here, we generate transgenic mice expressing a mitochondrial matrix-targeted ascorbate peroxidase (MTS-APEX2) to enable analysis...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-08-31 | PXD026793 | Pride
Coenzyme q Proximity labeling Mitochondrial proteome Apex Rtn4ip1 Nadph Muscle Lc-ms/ms 
ProteomicsMechanisms of Chemotherapy Failure in Refractory/Relapsed Acute Myeloid Leukemia: The Role of Cytarabine Resistance and Mitochondrial Metabolism
Acute myeloid leukemia (AML) originates from malignant, immature myeloid progenitor cells that differentiate into dysfunctional myeloblasts. While cytarabine (Ara-C) and daunorubicin (DNR)-based chemotherapy regimens are the standard treatments, approximately 10-40% of AML patients under the age of ...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD054797 | Pride
Mitochondrial metabolism Acute myeloid leukemia Cytrabine 
ProteomicsSafe and Stable Germline transmission of MSTN Mutations in Cattle
This project evaluates the genetic and proteomic safety of MSTN gene-edited cattle. Using TMT-based LC-MS/MS, we profiled protein expression in muscle tissue and confirmed stable inheritance and minimal off-target effects, supporting the viability of gene editing for livestock productivity.
ORGANISM(S): Bos taurus (Bovine) 
2026-09-14 | PXD065679 | Pride
Longissimus dorsi quantitative proteome Lc-ms3 Bovine muscle tissue Mstn-mutated cattle mass spectrometry Tmt-based proteomics 
ProteomicsUnbiased characterization and systematic profiling of metabolically labeled RNA-binding sites
Here we introduce pRBS-ID, an amenable platform that enables robust profiling of PAR-crosslinked RBSs. Using tandem mass spectrometry (MS/MS) approaches, we unbiasedly characterize the PAR adducts on peptides, to develop an optimal MS/MS search pipeline that enables robust identification and quantif...
ORGANISM(S): Homo sapiens (Human) 
2021-11-02 | PXD023401 | Pride
Human Lc-ms/ms 
ProteomicsMaximally-multiplexed proteome quantification platform for isotopic metabolic and chemical labeling
Quantitative proteomic platforms based on precursor intensity in mass spectrometry (MS1-level) uniquely support in vivo metabolic labeling with superior quantification accuracy but suffers from limited multiplexity (≤3-plex) and frequent missing quantities. Herein, we present a maximally-multiplexed...
ORGANISM(S): Homo sapiens (Human) 
2020-03-19 | PXD009952 | Pride
Diethylation Heat shock Silac-6plex Protein quantification Hela cell Quantification Silac De-6plex Heat shock response 
ProteomicsFormaldehyde-crosslinking enables comprehensive profiling of in vivo RNA-interactome in UV-impermeable samples
Development of UV-crosslinking based RNA-interactome capture protocol have expanded the repertoire of RBPs. Apparent limitations of UV-crosslinking, however, have made the RBP profiling to be incomplete and inapplicable to many of the biological samples in physiological contexts. Here we developed f...
ORGANISM(S): Homo sapiens (Human) Xenopus laevis (African clawed frog) 
2020-11-25 | PXD010046 | Pride
Rbp formaldehyde 
ProteomicsEpigenetic resetting in the Parkinson's disease entail histone modification remodeling.
Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by the loss of dopaminergic neurons (DN) in the substantia nigra and disrupted cellular maintenance. Here, we demonstrate that NDST3-mediated epigenetic reprogramming halts neuronal degeneration and restores motor...
ORGANISM(S): Homo sapiens (Human) 
2026-03-16 | PXD067971 | Pride
Human Tmt Parkinson's Organoid Lc-ms/ms 
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