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ProteomicsHighly reproducible improved label-free quantitative analysis of cellular phosphoproteome by optimization of LC-MS/MS gradient and analytical column construction
Expanding the sequencing depth of the peptides showing a statistically significant quantitative change arising from a biological stimulation is critical. Here we demonstrate that optimization of LC gradient and analytical column construction can reveal over 30,000 unique peptides and 23,000 phosphop...
ORGANISM(S): Homo sapiens (Human) 
2018-10-26 | PXD005650 | Pride
Human T cell Acute leukemia Q exactive Phosphoproteomics Label free 
ProteomicsLabel-free quantitative phosphoproteomics with novel pairwise abundance normalization reveals synergistic RAS and CIP2A signaling
Label-free quantification is a powerful method for studying cellular protein phosphorylation dynamics. However, whether current data normalization methods achieve sufficient accuracy has not been examined systematically. Here, we demonstrate that a large uni-directional shift in the phosphopeptide ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080775 | MassIVE
UnknownPhosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer.
Not available
S-EPMC6126612 | biostudies-literature
ProteomicsDiscovery of Mouse Spleen Signaling Responses to Anthrax using Label-Free Quantitative Phosphoproteomics via Mass Spectrometry
From article abstract: Inhalational anthrax is caused by spores of the bacterium Bacillus anthracis (B. anthracis), and is an extremely dangerous disease that can kill unvaccinated victims within 2 weeks. Modern antibiotic-based therapy can increase the survival rate to ~50%, but only if administere...
ORGANISM(S): Bacillus anthracis str. Sterne Mus musculus (Mouse) 
2017-03-22 | PXD006148 | Pride
Lc-msms Phosphoproteomics Mouse Label-free Spleen Anthrax 
UnknownHighly reproducible improved label-free quantitative analysis of cellular phosphoproteome by optimization of LC-MS/MS gradient and analytical column construction.
Not available
S-EPMC5542054 | biostudies-literature
ProteomicsProbing the global kinome and phosphoproteome in Chlamydomonas reinhardtii via sequential enrichment and quantitative proteomics
Identifying dynamic protein phosphorylation events is critical for understanding kinase/phosphatase regulated signaling pathways. To date, protein phosphorylation and kinase expression have been examined independently in photosynthetic organisms. Presented here is a method to study the global kinome...
ORGANISM(S): Chlamydomonas reinhardtii 
2016-10-05 | PXD004681 | Pride
Phosphoproteomics Quantitative label-free proteomics Kinome Mass spectrometry Chlamydomonas reinhardtii 
UnknownIdentification of Phosphorylated Cyclin-Dependent Kinase 1 Associated with Colorectal Cancer Survival Using Label-Free Quantitative Analyses.
Not available
S-EPMC4934865 | biostudies-literature
ProteomicsLabel-free quantitative phosphoproteomics with novel pairwise abundance normalization reveals synergistic RAS and CIP2A signaling
Label-free quantification is a powerful method for studying cellular protein phosphorylation dynamics. However, whether current data normalization methods achieve sufficient accuracy has not been examined systematically. Here, we demonstrate that a large uni-directional shift in the phosphopeptide ...
ORGANISM(S): Homo sapiens (Human) 
2015-08-24 | PXD001374 | Pride
Mascot Phosphoproteomics Cip2a Hela Okadaic acid Ras Simspectrast Lc-ms/ms 
ProteomicsA systematic comparison of label-free, SILAC and TMT techniques to study early adaption towards inhibition of EGFR signaling in the colorectal cancer cell line Difi
The epithelial growth factor receptor (EGFR) signalling network plays an essential role inproliferation and survival of colorectal cancer cells. For this reason, EGFR is targeted using therapeutic antibodies blocking EGFR signalling (e.g. cetuximab). However, anti-EGFR therapy is rarely curative as ...
ORGANISM(S): Homo sapiens (Human) 
2019-12-11 | PXD014565 | Pride
Phosphoproteomics Tmt Anti-egfr therapy Colorectal cancer Silac Mapk Label-free Erbb3 Adaptive mechanism Cetuximab 
ProteomicsLabel-Free Phosphoproteomics Profiling of Fission Yeast Schizosaccharomyces pombe
The phosphorylation of proteins modulates various functions of proteins and plays an important role in regulation of cell signaling. In the recent years, the label-free quantitative (LFQ) phos-phoproteomics has become the powerful tool to analyze the phosphorylation of proteins within the complex sa...
ORGANISM(S): Schizosaccharomyces pombe 927 
2021-02-12 | PXD023818 | Pride
Pgc Schizosaccharomyces pombe Sax Lfq phosphoproteomics 
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