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ProteomicsLow-melting point agarose as embedding medium for MALDI mass spectrometry imaging and laser-capture microdissection-based proteomics
We report an investigation of low-temperature embedding media in terms of their mechanical properties and embedding temperature, with the goal of determining a reliable embedding medium for the combination of MALDI MSI of lipids, laser-capture microdissection of tissue regions, and quantitative prot...
ORGANISM(S): Mus musculus (Mouse) 
2024-01-26 | PXD045349 | Pride
Proteomics Laser-capture microdissection Maldi msi Embedding media 
ProteomicsSpectral histopathology supported laser capture microdissection and quantitative proteome analysis of diffuse malignant mesothelioma
FTIR based spectral histopathology was used for label-free classification of tumor tissue sections. The regions of interest were identified and annotated with high spatial resolution. The spatial information was used for automated laser capture microdissection of relevant tissue areas. Subsequently,...
ORGANISM(S): Homo sapiens (Human) 
2017-03-31 | PXD004818 | Pride
Diffuse malignant mesothelioma Ftir Colorectal carcinoma Laser capture microdissection Infrared spectral histopathology 
ProteomicsLaser-capture proteomics of human FFPE substantia nigra
Laser-capture microdissection (LCM) allows the visualization and isolation of morphologically distinct subpopulations of cells from heterogeneous tissue specimens. In combination with formalin-fixed and paraffin-embedded (FFPE) tissue it provides a powerful tool for retrospective and clinically rele...
ORGANISM(S): Homo sapiens (Human) 
2020-03-05 | PXD016563 | Pride
Laser-capture microdissection Tmt Ffpe Substantia nigra 
UnknownLaser Capture Proteomics: spatial tissue molecular profiling from the bench to personalized medicine.
Not available
S-EPMC10720974 | biostudies-literature
ProteomicsProteomics on formalin-fixed and stained laser-capure microdissected human lung sections
A broad range of tissues have been examined by microscopy following a process of formalin fixation, paraffin embedment, sectioning and staining. Haematoxylin and eosin (H&E) – which respectively stain nuclei blue and other cellular and stromal material pink – are routinely used for clinical diagnosi...
ORGANISM(S): Homo sapiens (Human) 
2021-09-08 | PXD014762 | Pride
Lung Human Laser-capture Dissection Spatial proteomics Fixed 
ProteomicsProteomic analysis of matrisome proteins from laser capture microdissected FFPE tissue slides of HnSCC xenograft
Liquid chromatography mass spectrometry was used to study tumor matrisome from laser capture microdissected FFPE tissue sections of human neck and squamous cell carcinoma (HnSCC) xenograft grown in mouse.
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2023-10-21 | PXD033023 | Pride
Tumor matrisome Xenograft Laser capture microdissection 
ProteomicsMALDI informed microdissected airway proteomics
After performing MALDI metabolomics analysis, we identified airway regions. On a consecutive slide we used laser capture microdissection (LCM) to cut out regions of interest that had an airway specific signal by MALDI. The samples were prepped using the MicroPOTS protocol (PMID: 33491460) and analyz...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-10-20 | MSV000090561 | MassIVE
ProteomicsSampling and homogenization of nanoliter tissue with a nanosecond infrared laser (NIRL) for mass spectrometric proteomics
It was recently shown that ultrashort pulse infrared (IR) lasers, operating at the wavelength of the OH vibration stretching band of water, are highly efficient at softly sampling and homogenizing biological tissue. We utilized a nanosecond infrared laser (NIRL) for tissue sampling and homogenizatio...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-17 | PXD027700 | Pride
Laser ablation Tissue sampling  nanosecond infrared laser Proteomics  mass spectrometry 
ProteomicsQuantitative Proteomics Analysis of Laser Capture Microdissected Alveolar Tissue Samples
Proteomics characterization of the ontogeny of protein changes during normal lung development in laser capture micro-dissected alveolar tissue containing ~4,000 cells per sample
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2016-06-23 | MSV000079850 | MassIVE
ProteomicsCoupling microdroplet-based sample preparation, multiplexed isobaric labeling, and nanoflow peptide fractionation for deep proteome profiling of tissue microenvironment
Existing spatially-resolved proteomics technologies cannot provide deep proteome coverage due to limited sensitivity and poor sample recovery. Herein, we seamlessly combined laser capture microdissection with a low-volume sample processing technology that includes a microfluidic device named microPO...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-01-25 | MSV000093925 | MassIVE
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