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ProteomicsEssential role of translation factor eIF5a in cytokine and cell cycle regulation in primary mouse CD8 T lymphocyte
Translation of specific mRNAs is tightly regulated in primary T cells to ensure correct timing and precise delivery of protein syntheses in response to environmental cues. Translation factor eIF5a is known to be important for protein synthesis and is expected to regulate translation of certain diffi...
ORGANISM(S): Mus musculus (Mouse) 
2022-11-11 | PXD021063 | Pride
Cytokine Lymphocyte Hypusine Proliferation Eif5a 
ProteomicsCD8 T lymphocytes deploy embryonic cell cycle control mechanisms for rapid cell proliferation
Rapid proliferation of CD8 T cells is crucial for adaptive immunity against viral infection. CD8 T cells can complete division cycles in less than 6 hours, representing a physiological extreme for somatic mammalian cells. Embryonic stem cells utilize specialized cell cycle control mechanisms, includ...
ORGANISM(S): Mus musculus (Mouse) 
2026-09-14 | PXD077442 | Pride
Proteomics Mouse cell lines Dia Primary cells 
ProteomicsCyclin A triggers Mitosis either via Greatwall or Cyclin B
Two mitotic Cyclins, A and B, exist in higher eukaryotes, but their specialised functions in mitosis are poorly understood. Using degron tags we analyse how acute depletion of these proteins affects mitosis. Loss of Cyclin A in G2-phase prevents the initial activation of Cdk1. Cells lacking Cyclin B...
ORGANISM(S): Homo sapiens (Human) 
2020-05-21 | PXD012100 | Pride
Human Phosphoproteomics Cyclin Cell cycle Mitosis 
ProteomicsProteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS
The temporal regulation of protein abundance and post-translational modifications is a key feature of cell division. Recently, we analysed gene expression and protein abundance changes during interphase under minimally perturbed conditions (Ly et al. 2014; Ly et al. 2015). Here we show that by using...
ORGANISM(S): Homo sapiens (Human) 
2017-10-18 | PXD007787 | Pride
Human Facs Cell cycle 
ProteomicsCDK4/6 inhibitors induce repication stress to cause long-term cell cycle withdrawal
CDK4/6 inhibitors arrest the cell cycle in G1-phase. They are licenced to treat breast cancer and are also undergoing clinical trials against a range of other tumour types. To facilitate these efforts, it is important to understand why a temporary cell cycle arrest in G1 causes long-lasting effects ...
ORGANISM(S): Homo sapiens (Human) 
2021-12-20 | PXD023435 | Pride
Cdk4/6 cancer replication stress cell cycle senescence 
ProteomicsCDK4/6 inhibitor-mediated cell overgrowth triggers an osmotic stress response
Cell size and the cell cycle are intrinsically coupled and abnormal increases in cell size are associated with senescence and permanent cell cycle arrest. The mechanism by which overgrowth primes cells to withdraw from the cell cycle remains unknown. We investigate this here using CDK4/6 inhibitors ...
ORGANISM(S): Homo sapiens (Human) 
2023-11-27 | PXD036519 | Pride
Lc-ms/ms Dia Cell growth Cell cycle 
ProteomicsProteomic Analysis of the Response to Cell Cycle Arrests in Human Myeloid Leukemia Cells
Previously (Ly et al. 2014), we analyzed protein abundance changes across a ‘minimally perturbed’ cell cycle in human cells (NB4), using centrifugal elutriation to differentially enrich distinct cell cycle phases. Here, we compare data from elutriated cells with NB4 cells arrested at comparable ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080841 | MassIVE
ProteomicsThe chromatin assembly factor complex 1 (CAF1) and 5-Azacytidine (5-AzaC) affect cell motility in Src-transformed human epithelial cells
Tumor invasion into surrounding stromal tissue is a hallmark of high-grade, metastatic cancers. Oncogenic transformation of human epithelial cells in culture can be triggered by activation of v-Src kinase, resulting in increased cell motility, invasiveness and tumorigenicity and provides a valuable ...
ORGANISM(S): Homo sapiens (Human) 
2016-12-20 | PXD005494 | Pride
Epigenetics Proteomics Invasion 
ProteomicsHs_GlobalSubcellular_SILAC
Characterisation of PRR11
ORGANISM(S): Homo Sapiens (human) 
2013-12-31 | PAe005033 | PeptideAtlas
ProteomicsSCF-Fbxo42 promotes synaptonemal complex assembly by downregulating PP2A-B56
Meiosis creates genetic diversity by recombination and segregation of chromosomes. The synaptonemal complex assembles during meiotic prophase I and assists faithful exchanges between homologous chromosomes, but how its assembly/disassembly is regulated remains to be understood. Here we report how ...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2020-12-07 | PXD022755 | Pride
Drosophila 
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