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ProteomicsNicotinamide Mononucleotide Requires SIRT3 to Improve Cardiac Function and Bioenergetics in a Friedreich’s Ataxia Cardiomyopathy Model
Martin et al. JCI Insigh (2017). Increasing NAD+ levels by supplementing with the precursor nicotinamide mononucleotide (NMN) improves cardiac function in multiple mouse models of disease. While NMN influences several aspects of mitochondrial metabolism, the molecular mechanisms by which increased N...
ORGANISM(S): Mus musculus (Mouse) 
2017-07-21 | PXD006754 | Pride
Nmn Friedreich’s ataxia Heart failure Sirt3 Acetylation 
ProteomicsProximity biotinylation of CDK1 associated proteins in U2OS cells
BirA ligase tagged CDK1 and BirA alone were expressed in U2OS cells. Proximal proteins to CDK1 were labelled by incubating cells with Biotin for 24 hours and biotinylated proteins extracted using Streptavidin pulldown.
ORGANISM(S): Homo sapiens (Human) 
2021-05-12 | PXD024796 | Pride
Human Lc-msms Proximity biotinylation Cdk1 
ProteomicsU2OS Adhesion Isolations +/- CytochalasinD and Neomycin
U2OS cells preferentially utilise integrin alphaV beta5 in adhesion complexes when grown under standard cell culture conditions for 3 days. AlphaV beta5 can be found in both classical 'Focal' adhesions and in novel 'Reticular' adhesions that do not associate with F-actin or other known adhesion prot...
ORGANISM(S): Homo sapiens (Human) 
2019-06-03 | PXD008680 | Pride
Human Cytod Reticular U2os Proteomics 
ProteomicsHeLa and U2OS long term culture adhesion isolations
Integrin adhesion complexes have been previously isolated and analysed by mass-spectrometry from cells largely spread on matrix proteins over a couple of hours. Here we wished to determine the composition of adhesion complexes from cells cultured for 72 hours under standard cell culture conditions a...
ORGANISM(S): Homo sapiens (Human) 
2019-06-03 | PXD008645 | Pride
Human Cancer cell Proteomics Integrin Adhesion complex Cell culture 
TranscriptomicsInhibition of endogenous MTF-1 signaling in zebrafish embryos identifies novel roles for MTF-1 in development
The MTF-1 transcription factor is considered to be a master regulator of zinc homoestasis. We previously characterized a constitutively nuclear, dominant-negative zebrafish MTF-1/eGFP fusion protein (dnMTF-1). In this study, in vitro transcribed dnMTF-1 mRNA was microinjected into zebrafish embryos ...
ORGANISM(S): Danio rerio 
2014-04-25 | E-GEOD-51298 | biostudies-arrayexpress
TranscriptomicsThe role of carbon starvation in the induction of enzymes that degrade plant-derived carbohydrates in Aspergillus niger
Fungi are an important source of enzymes for saccharification of plant polysaccharides and production of biofuels. Understanding of the regulation and induction of expression of genes encoding these enzymes is still incomplete. To explore the induction mechanism, we analysed the response of the indu...
ORGANISM(S): Aspergillus niger 
2014-08-22 | E-GEOD-57315 | biostudies-arrayexpress
TranscriptomicsRNA-Seq based characterization of long non-coding RNA involved in respiratory viruses pathogenesis
We aimed at systematically inferring the regulatory functions of host lncRNAs in response to influenza A virus (IAV) and severe acute respiratory syndrome coronavirus (SARS-CoV) in the mouse model, using a ‘guilt-by-association’ approach which relies on finding which lncRNAs have similar expression ...
ORGANISM(S): Mus musculus 
2014-05-05 | E-GEOD-52405 | biostudies-arrayexpress
GenomicsA SNF2 protein targets variable copy number repeats and thereby influences allele-specific expression
ATRX is an X-linked gene of the SWI/SNF family whose role in vivo is currently unknown. Mutations in ATRX cause syndromal mental retardation. ATRX binds to tandem repeat (TR) sequences both in heterochromatin (e.g. telomeres) and euchromatin. Genes associated with these TRs can be dysregulated when ...
ORGANISM(S): Homo sapiens 
2010-11-02 | E-GEOD-22162 | biostudies-arrayexpress
UnknownIncidence, aetiology, and sequelae of viral meningitis in UK adults: a multicentre prospective observational cohort study.
Not available
S-EPMC6105576 | biostudies-literature
ProteomicsAlzheimer's Disease Isomerization
Amino acid isomerization is a spontaneous chemical modification potentially related to the underlying causes of Alzheimer’s disease (AD). We demonstrate that data-independent acquisition mass spectrometry can be used to characterize isomerization in complex protein mixtures. Examination of a large c...
ORGANISM(S): Homo Sapiens 
2022-03-16 | PXD025668 | panorama
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