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ProteomicsDynamics of allosteric regulation of the phospholipase C-gamma isoform
we used hydrogen deuterium exchange masss spec (HDX-MS) to define the exchange kinetics of PLC-y1 as it interacts with the kinase domain of FGFR1, and liposomes containing PIP2, in order to understand how PLC-y isozymes operate at membranes. We show that the binding of FGFR1k to PLC-y1 increases deu...
ORGANISM(S): Rattus norvegicus (Rat) 
2022-06-14 | PXD030492 | Pride
Hdxms Plc 
ProteomicsExtreme MetaboHealth scores in three cohort studies associate with plasma protein markers for inflammation and cholesterol transport
The MetaboHealth score is a highly informative health indicator in ageing studies and yet contains only a small number of metabolites. Here we estimate the heritability of the score in 726 monozygotic (MZ) and 450 dizygotic (DZ) twin pairs, and test for association with plasma proteins by comparing ...
ORGANISM(S): Homo sapiens (Human) 
2025-09-15 | PXD057946 | Pride
Plasma proteomics Metabolomics Aging Metabohealth scores Masss spectrometry Cytokines Immunoassay 
Proteomicsphospho-proteomic data on the LATS1 interactome from cells treated with two proapoptotic signals: FAS and etoposide
Using this approach, we obtained phospho-proteomic data on the LATS1 interactome from cells treated with two proapoptotic signals: FAS and etoposide, which both activate LATS kinase activity [28].To identify the LATS1 interactome we transiently expressed GFP-LATS1 in HeLa cells, immunoprecipitated G...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD018903 | Pride
Human Lats1 Hela 
ProteomicsCarrier protein-enabled host cell proteome analysis (CP-HCP) with integrated isobaric tag interference filtering.
Here, we proposed that the use of an HCP carrier proteome could enhance the acquisition of spectra from HCPs amidst a highly abundance therapeutic protein background to perform global HCP analysis. We provide proof of principle of this approach, which we term carrier proteome-HCP (CP-HCP) analysis, ...
ORGANISM(S): Escherichia coli Bacteria 
2026-01-12 | PXD049386 | Pride
Carrier proteome Host cell proteome E.coli 
ProteomicsPlasma membrane-derived extracellular microvesicles mediate non-canonical intercellular NOTCH signaling
ARMMs (arrestin domain-containing protein 1 [ARRDC1]-mediated microvesicles) are extracellular vesicles that bud directly atthe plasma membrane. However, little is known about the physiological function of these vesicles. Here we report that secreted ARMMs contain active NOTCH receptors and mediate ...
ORGANISM(S): Homo sapiens (Human) 
2017-10-11 | PXD006416 | Pride
Armms Notch Arrdc1 
ProteomicsBenchmarking of different methods for obtaining high quality secretomics data
The main drawback of secretome analyses are contaminating proteins derived from culture medium or proteins released from dying cells which are hard to distinguish from proteins actively secreted by cells. Therefore, several approaches have been applied allowing to remove contamination from secretome...
ORGANISM(S): Homo sapiens (Human) 
2020-10-19 | PXD018895 | Pride
Supernatant Secretomics Secreteome Benchmarking Conditioned medium 
MetabolomicsMapping immune variation and var gene switching in naive hosts infected with Plasmodium falciparum
Falciparum malaria is clinically heterogeneous and the relative contribution of parasite and host in shaping disease severity remains unclear. We explored the interaction between inflammation and parasite variant surface antigen (VSA) expression, asking whether this relationship underpins the variat...
2020-12-02 | MTBLS1188 | MetaboLights
ProteomicsTranslational regulation contributes to the secretory response of chondrocytic cells following exposure to Interleukin-1B
Chondrocytes undergo changes to their protein translational capacity during osteoarthritis progression, but a study of how disease-relevant signals affect chondrocyte protein translation at the transcriptomic level has not previously been performed. In this study we describe how the inflammatory cyt...
ORGANISM(S): Homo sapiens (Human) 
2019-09-27 | PXD011740 | Pride
Human Interleukin-1b Secretion Chondrocytes Inflammation Ribosome profiling 
ProteomicsMsc1 is a novel nuclear envelope protein that reinforces the DNA damage response in late mitosis
Precise double-strand break (DSB) signaling and repair is paramount for maintaining genome stability. Homologous recombination (HR) is the chosen DSB repair pathway when cyclin-dependent kinase (CDK) activity is high, as it correlates well with the availability of an intact sister chromatid to be us...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-08-10 | PXD043515 | Pride
Lc-ms/ms 
ProteomicsAccurate Prediction of Kinase-Substrate Networks Using Knowledge Graphs
Phosphorylation of specific substrates by protein kinases is a key control mechanism for vital cell-fate decisions and other cellular processes. However, discovering specific kinase-substrate relationships is time-consuming and often rather serendipitous. Computational predictions alleviate these ch...
ORGANISM(S): Homo sapiens (Human) 
2022-02-20 | PXD018905 | Pride
Statistical relational learning Cell signaling Kinase-substrate predictions Machine learning Linkphinder 
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