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Previous systems-based proteomic approaches have characterized alterations in protein co-expression networks of unfractionated asymptomatic (AsymAD) and symptomatic Alzheimer’s disease (AD) brains. However, it remains unclear how sample fractionation and sub-proteomic analysis influences the organi...
ORGANISM(S): Homo sapiens (Human) 
2019-09-02 | PXD014376 | Pride
Pollen germination and subsequent pollen tube elongation are essential for successful land plant reproduction. These processes are achieved through well-documented activation of membrane trafficking and cell metabolism. Despite this, our knowledge of the dynamics of cellular phospholipids remains sc...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2022-10-27 | PXD037046 | Pride
Cellular lipid metabolism is subject to strong homeostatic regulation, but players involved in and mechanisms underlying these pathways remain mostly uncharacterized. Here, we develop and exploit a “Feeding-Fishing” approach coupling membrane editing using optogenetic lipid-modifying enzymes (feedin...
ORGANISM(S): Homo sapiens (Human) 
2026-02-23 | PXD070378 | Pride
Membrane lipids and their metabolism have key functions in neurotransmission. Here we provide a quantitative lipid inventory of mouse and rat synaptic junctions. To this end, we developed a multiomics extraction and analysis workflow to probe the interplay of proteins and lipids in synaptic signal t...
2021-09-27 | MTBLS1531 | MetaboLights
Cellular lipid metabolism is subject to strong homeostatic regulation, but players involved in and mechanisms underlying these pathways remain mostly uncharacterized. Here, we develop and exploit a “Feeding-Fishing” approach coupling membrane editing using optogenetic lipid-modifying enzymes (feedin...
ORGANISM(S): Homo sapiens (Human) 
2026-02-23 | PXD070270 | Pride
Synaptosome (Syn) and synaptic junctions (SJ) are widely used in omics experiments to investigate protein content and networks of synapses. Synapses are a neuronal compartment wit a high lipid content. Liquid-liquid extraction (LLE), typically used in lipidomics, shows great potential for multiomics...
ORGANISM(S): Rattus rattus (Black rat) 
2025-09-08 | PXD058138 | Pride
This study used subcellular fractionation combined with proteomics for a comprehensive overview of the protein localizations of R. salmoninarum proteins within enriched subcellular fractions of cytosolic, membrane, extracellular and membrane vesicle (MV) proteins. With this approach, 1,674 protein g...
ORGANISM(S): Renibacterium salmoninarum ATCC 33209 
2022-02-24 | PXD025586 | Pride
Abstract of associated manuscript: Daptomycin is the first of a new class of cyclic lipopeptide antibiotics used against multidrug-resistant Gram-positive pathogens. The proposed mechanism of action involves disruption of the functional integrity of the bacterial membrane in a Ca2+-dependent manner....
ORGANISM(S): Bacillus subtilis 
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