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ProteomicsA microsomal proteomics view of H2O2- and ABA-dependent responses
The plant hormone abscisic acid (ABA) modulates a number of plant developmental processes and responses to stress. In planta, ABA has been shown to induce reactive oxygen species (ROS) production through the action of plasma membrane-associated NADPH oxidases. Although quantitative proteomics studie...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-08-22 | PXD006513 | Pride
Hydrogen peroxide (h2o2)  abscisic acid (aba)  microsomal proteomics  quantitative proteomics  mass spectrometry 
ProteomicsSimple Strategy of Integrating the Micro-centrifugation-based Extraction and 4-Hexylphenylazosulfonate for the Preparation of Plant Microsomal Proteins for Bottom-Up Proteomics
The preparation of high-quality microsomal proteins (MPs) is of critical importance to high-throughput microsomal proteomics. The isolation of MPs for proteomic analysis has to date relied on Ultracentrifugation-based methods (UBMs), however, these methods are labor-intensive, time-consuming, and un...
ORGANISM(S): Oryza sativa subsp. japonica (Rice) 
2021-09-29 | PXD025132 | Pride
Rice leaves Perseus Label-free quantitative proteomics Ultracentrifugation Micro-centrifugation Azo Maxquant Microsomal membrane extraction Plasma membrane Sds 
ProteomicsRegulation of the microsomal proteome by salicylic acid and deficiency of phosphatidylinositol‐4‐kinases β1 and β2 in Arabidopsis thaliana
Phosphatidylinositol-4-kinases β1 and β2 (PI4Kβ1/PI4Kβ2), which are responsible for phosphorylation of phosphoinositol to phosphatidylinositol-4-phosphate, have an important role in vesicular trafficking in plants. Moreover, PI4Kβ1/PI4β2 negatively regulate biosynthesis of phytohormone salicylic aci...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-02-24 | PXD019737 | Pride
Arabidopsis thaliana Microsomal fraction Phosphatidylinositol-4-kinases β1 and β2 Salicylic acid Label-free quantification Lc-ms/ms 
ProteomicsConsequences of Cyp2c44 deletion on proteome of the microsomal fraction of primary polyoma middle T (PyMT) tumors
Here we compared the proteomes of microsomal fractions from primary tumors isolated from PyMT mice versus PyMTxCyp2c44-/- mice.
ORGANISM(S): Mus musculus (Mouse) 
2020-03-03 | PXD017303 | Pride
Breast cancer Macrophage Wdfy1 Lymphangiogenesis Lc-msms Metastasis 
UnknownProteomic Profiling of the Microsomal Root Fraction: Discrimination of Pisum sativum L. Cultivars and Identification of Putative Root Growth Markers.
Not available
S-EPMC5372229 | biostudies-literature
UnknownQuantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry.
Not available
S-EPMC1444949 | biostudies-literature
ProteomicsSubcellular Proteomic Analyses Reveal REEP5 Knockdown in the Mouse Heart Disrupts Mitochondrial Networks
This study investigates the role of REEP5 in maintaining organelle integrity and redox homeostasis in cardiac tissue (specifically cardiomyocytes). Using AAV9-mediated shRNA knockdown in postnatal mice, we acheived a cardiac-specific depletion of REEP5. Hearts were harvested 4 weeks post-injection a...
ORGANISM(S): Mus musculus (Mouse) 
2026-03-30 | PXD065280 | Pride
UnknownNatural Antioxidant By-Product Mixture Counteracts the Effects of Aflatoxin B1 and Ochratoxin A Exposure of Piglets after Weaning: A Proteomic Survey on Liver Microsomal Fraction.
Not available
S-EPMC10143337 | biostudies-literature
UnknownMicrosomal monooxygenase as a multienzyme system: the role of P450-P450 interactions.
Not available
S-EPMC3079778 | biostudies-literature
ProteomicsDifferentially expressed proteins in benign and malignant adrenocortical tumors
We have compared the microsomal protein fractions from benign and malignant adrenocortical tumors. Protein extracts were trypsinized, peptides separated by HiRIEF (high resolution isoelectric focusing) and analysed by LC-MS.
ORGANISM(S): Homo sapiens (Human) 
2014-02-11 | PXD000604 | Pride
Microsomal fraction Hirief Lc-ms Isoelectric focusing Adrenocortical tumors Cancer 
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