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TranscriptomicsHeLa cell mitochondrial microRNAs
MicroRNAs (miRNAs) are small non-coding RNAs that associate with Argonaute 2 protein to regulate gene expression at the post-transcriptional level in the cytoplasm. However, recent studies have reported that some miRNAs localize to and function in other cellular compartments. Mitochondria harbour th...
ORGANISM(S): Homo sapiens 
2011-10-05 | E-GEOD-24761 | biostudies-arrayexpress
TranscriptomicsLooking for mitochondrial associated RNA
We investigated the peripheral mitochondrial localization of nuclear-encoded mRNAs (MLR) in various conditions in which translation was inhibited or the mRNA binding protein context altered (Delta puf3). We used cell fractionation protocols together with microarray to assess the distribution of mRNA...
ORGANISM(S): Saccharomyces cerevisiae 
2008-06-01 | E-GEOD-9393 | biostudies-arrayexpress
ProteomicsC1QBP mitochondrial interactome
The study of protein-protein interactions is an essential process to understand the biological functions of proteins and the underlying mechanisms. Co-immunoprecipitation coupled with mass spectrometry (CoIP-MS) is one of the most extensively used high-throughput techniques to discover novel protein...
ORGANISM(S): Homo Sapiens 
2015-11-04 | MSV000079369 | MassIVE
TranscriptomicsBiochemical fractionation of HEK293 nuclei and RNA-seq of chromatin-associated and soluble-nuclear RNA
Biochemical fractionation of HEK293 nuclei and RNA-seq of chromatin-associated and soluble-nuclear RNA. Nuclei from three biological replicates were isolated by detergent lysis, fractionated, then three chromatin and three soluble RNA samples were converted to cDNA using Illumina TruSeq stranded pro...
ORGANISM(S): Homo sapiens 
2015-03-04 | E-GEOD-66478 | biostudies-arrayexpress
ProteomicsSequential Fractionation Strategy Identifies Nine Missing Proteins in The Mitochondrial Proteome of Commonly Used Cell Lines
Identification of mitochondrial proteins with a bottom up approach after organelle enrichment and 1D PAGE separation for 5 different cell lines. The bands were cut and the proteins reduced, alkylated and digested with trypsin according to a conventional protocol. Acquisition were performed on the Or...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-05-28 | MSV000082409 | MassIVE
ProteomicsMYC deregulation sensitizes cancer cells to N-myristoyltransferase inhibition - Mitochondrial fractionation
Human N-myristoyltransferases (NMTs) catalyze N-terminal protein N myristoylation and are promising targets in cancer, with an emerging mechanistic rationale for targeted therapy. Here, we screened 245 cancer cell lines against IMP-1320, a potent NMT inhibitor (NMTi), and conduced pathway-level anal...
ORGANISM(S): Homo sapiens (Human) 
2025-07-30 | PXD056941 | Pride
N-myristoyltransferase Proteomics Mitochondrial fractionation N-myristoylation 
TranscriptomicsHuman mitochondria hub of small RNAs: Analysis by deep Sequencing
We report here that human mitochondria contain small RNA including microRNA, piRNA, tRNA, rRNA, and RNA repeats. Mitochondria from human cells were purified and RNA isolated. Small RNAs were purified, library generated and analyzed by Illumina Hiseq 2000 system. The sequencing generated 19.5 and 17....
ORGANISM(S): Homo sapiens 
2013-03-05 | E-GEOD-32185 | biostudies-arrayexpress
ProteomicsSIRT3 interactions and mitochondrial proteome during HCMV infection
SIRT3 has been shown to inhibit HCMV infection, but the mechanism under the antiviral effect is not clear. To identify the mediator of SIRT3 antiviral function, we aim to identify and quantify SIRT3 interactions during HCMV infection. A set of large-scale IPs were performed to determine the specific...
ORGANISM(S): Homo sapiens (Human) 
2021-04-01 | PXD018995 | Pride
Hcmv Mitochondrial proteome Ip-ms Sirt3 
ProteomicsSILAC-based subcellular fractionation (PM,Golgi,soluble) upon DNAJC15 and DNAJC19 knockdown in HeLa cells
Mitochondria adapt to cellular stress to ensure cell survival. The stress-regulated mitochondrial peptidase OMA1 orchestrates these adaptive responses, which limit mitochondrial fusion and promote mitochondrial stress signaling and metabolic rewiring. Here, we show that cellular stress adaptation in...
ORGANISM(S): Homo sapiens (Human) 
2025-12-16 | PXD061185 | Pride
Dnajc19 Hela Silac Dnajc15 Subcellular 
UnknownRapid fractionation of mitochondria from mouse liver and heart reveals in vivo metabolite compartmentation.
Not available
S-EPMC7614208 | biostudies-literature
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