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MetabolomicsMetabolomic profiling of primary mouse proximal tubule cells (mPTCs) derived from 24-week-old CTR and Atg7 PT-KO kidneys
To explore the role of ATG7 in the kidney PT, we crossed mice homozygous for floxed alleles of Atg7 with Ggt1-Cre mice expressing Cre recombinase under the rat Ggt1 (gamma-glutamyltransferase 1) gene promoter. This generated Atg7fl/fl; γGt1cre/cre mice (hereafter referred to as Atg7 PT-KO), in which...
2026-09-30 | MTBLS15857 | MetaboLights
ProteomicsUnique insights in cervicovaginal Lactobacillus iners and L. crispatus proteome changes associated with microbiome dysbiosis
Cervicovaginal lavage (CVL)supernatants were heat inactivated for 30 minutes at 56°C before further processing. Total protein concentrations were determined using the Pierce Coomassie Plus (Bradford) Protein Assay (Thermo Scientific, Rockford, IL, USA). Sample protein content and volume were normali...
ORGANISM(S): Homo sapiens (Human) Lactobacillus iners Lactobacillus crispatus 
2016-03-15 | PXD003176 | Pride
Label free proteomics Cervicovaginal microbiome dysbiosis 
ProteomicsHDX-MS analysis of the full human proteasome in complex with regulators
Hydrogen-Deuterium eXchange coupled to Mass Spectrometry (HDX-MS) analysis to investigate the solvent accessibility of the full human 20S proteasome alone or in complex with PA28 regulatory particles. Our work reveals a reciprocal crosstalk between the inner and outer rings of the human 20S proteaso...
ORGANISM(S): Homo sapiens (Human) 
2020-09-11 | PXD018921 | Pride
: proteasome Hydrogen-deuterium exchange Immunoproteasome Structural biology Pa28 alpha beta Bioinformatics Pa28 gamma Structural ms 
ProteomicsProteomic analysis of isolated integrin-associated adhesion complexes in Trastuzumab -sensitive and -resistant BT474 cells
Peptide analysis by LC-MS/MS was performed using a nanoACQUITY™ (Waters) Ultra Performance Liquid Chromatography (UPLC) column, coupled to an LTQ-Orbitrap XL (Thermo Fisher) equipped with a nanoelectrospray source (Proxeon).
ORGANISM(S): Homo sapiens (Human) 
2024-11-03 | PXD055603 | Pride
Adhesion Breast cancer Trastuzmab Itgb6 Erbb2 Her2 Herceptin 
ProteomicsProteomic Analysis of isolated integrin-associated adhesion complexes in BT474 cells
Peptide analysis by LC-MS/MS was performed using a nanoACQUITY™ (Waters) Ultra Performance Liquid Chromatography (UPLC) column, coupled to an LTQ-Orbitrap XL (Thermo Fisher) equipped with a nanoelectrospray source (Proxeon).
ORGANISM(S): Homo sapiens (Human) 
2024-11-03 | PXD055411 | Pride
Trastuzumab Adhesion Breast cancer Receptor trafficking Erbb2 Her2 
ProteomicsProteomic Analysis of isolated integrin-associated adhesion complexes in HER2-18 CELLS
Peptide analysis by LC-MS/MS was performed using a nanoACQUITY™ (Waters) Ultra Performance Liquid Chromatography (UPLC) column, coupled to an LTQ-Orbitrap XL (Thermo Fisher) equipped with a nanoelectrospray source (Proxeon).
ORGANISM(S): Homo sapiens (Human) 
2024-11-03 | PXD055405 | Pride
Trastuzumab Adhesion Breast cancer Receptor trafficking Itgb6 Erbb2 Her2 
ProteomicsQuantitation of plasma protein concentrations by mass spectrometry without the use of isotopic standards. C4PR_LIV
We applied a non targeted mass spectrometric assay (LCMSE), to quantify 55 abundant plasma proteins in specimens from 31 healthy volunteers. Quantitation was done by HI3 peptide measurement using a single internal standard to estimate protein concentrations. Results for 7 apolipoproteins were compar...
ORGANISM(S): Homo sapiens (Human) 
2015-10-20 | PXD000347 | Pride
Lcmse Quantitation Mass spectrometry Plasma proteins Proteomics Immunoassay 
UnknownSubzero Celsius separations in three-zone temperature controlled hydrogen deuterium exchange mass spectrometry.
Not available
S-EPMC5675777 | biostudies-literature
ProteomicsProteomics analysis of B. bigemina
Protein from B. bigemina PR cell lysates was analysed by 1D SDS PAGE and in solution digestion. Digested peptides were analysed by on-line nanoflow liquid chromatography using the nanoACQUITY-nLC system (Waters MS technologies, Manchester, UK) coupled to an LTQ-Orbitrap Velos (ThermoFisher Scientifi...
ORGANISM(S): Babesia bigemina Babesia sp. 
2014-06-16 | PXD000629 | Pride
Label free 1d sds page B. bigemina 
ProteomicsA Tandem Mass Tag (TMT) proteomic analysis during the early phase of experimental pancreatitis
We performed in a rat pancreatitic model a quantitative shotgun proteomic profiling of the soluble and whole membrane fractions from the pancreas during the early phase of cerulein (Cer)-induced AP compared to controls. Three control and three pancreatitic samples from both the soluble and the whole...
ORGANISM(S): Rattus norvegicus (Rat) 
2018-05-22 | PXD007096 | Pride
Cerulein Rat pancreas Subcellular fractions Acute pancreatitis Tmt (tandem mass tags) 
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