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ProteomicsMycobacterial strain-specific temporal modulations of newly synthesized macrophage secretome
THP-1 macrophages were infected with four strains of Mycobacterium tuberculosis to study the temporal dynamics of newly synthesized proteins in the secretome. Temporal snapshots of secretome reflect the macrophage response to pathogenicity which in combination with intracellular events, completes th...
ORGANISM(S): Homo sapiens (Human) 
2016-08-10 | PXD004281 | Pride
Boncat Mtb infection Hyperplexing Newly synthesized Silac Temporal Itraq Secretome 
ProteomicsAutomated method for enrichment of newly synthesized proteins based on AHA labeling
In this study, a novel high throughput method was successfully developed for the automated enrichment of newly synthesized proteins (NSPs) from complex environments, like cell culture media supplemented with fetal bovine serum (FBS). The proposed automated method allows for the robust and effective ...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD025255 | Pride
High throughput Newly synthesized proteins Aha Lc-ms Interferon Melanoma Enrichment Proteomics Click chemistry 
ProteomicsNewly synthesized proteome analysis of interferon gamma treated Hela cells
Using a newly-developed workflow for quantitative newly synthesized proteome analysis (QuaNPA), featuring automated sample processing and multiplexed DIA (plexDIA) analysis, changes in the newly synthesized proteome of IFN-gamma treated Hela cells were monitored over time.
ORGANISM(S): Homo sapiens (Human) 
2023-10-19 | PXD038915 | Pride
Dia Nascent proteome analysis Automated sample processing Interferon gamma Silac 
ProteomicsApplying plexDIA analysis to newly synthesized proteome samples generated using a semi-automated enrichment protocol
Samples generated with a newly development semi-automated enrichment protocol for newly synthesized proteins, using different amounts of protein input, were analysed using data-independent acquisition (DIA) and processed with the plexDIA software features of DIA-NN (https://www.nature.com/articles/s...
ORGANISM(S): Homo sapiens (Human) 
2023-10-19 | PXD043817 | Pride
Silac Plexdia Quanpa 
ProteomicsSystematic evaluation of protein synthesis inhibition through global quantification of newly synthesized protiens
Manipulating protein synthesis is commonly applied in bio-related research to uncover protein functions and cellular activities. Multiple inhibitors with distinct mechanisms are widely used, but it is extraordinarily challenging to systematically determine and evaluate their inhibition efficiencies,...
ORGANISM(S): Homo sapiens (Human) 
2023-05-24 | PXD035992 | Pride
Bioorhogonal chemistry  protein synthesis inhibtion  protein inhibition efficiency  mass spectromety  multiplexed proteomics  newly synthesized proteins 
Proteomicssemi-automated enrichment of AHA-containing newly-synthesized proteins with magnetic alkyne agarose beads
Development of an improved workflow for newly synthesized proteome analysis, based on combined pulsed metabolic labelling with L-azidohomoalanine (AHA) and semi-automated click chemistry-based enrichments with magnetic alkyne agarose beads.
ORGANISM(S): Homo sapiens (Human) 
2023-10-19 | PXD036886 | Pride
Automation Silac Click chemistry Nascent proteome 
ProteomicsNewly Synthesized protein after VapC5 induction in Mycobacterium abscessus ATCC19977
Analyzing newly synthesized protein after VapC5 induction in mycobacterium abscessus 19977
ORGANISM(S): Mycobacteroides abscessus subsp. abscessus 
2023-07-20 | PXD025047 | Pride
Lc-ms Mycobacterium abscessus atcc19977 
GenomicsIdentification of newly synthesized proteins in microbial communities using BONCAT and click chemistry
Identification of newly synthesized proteins in microbial communities using BONCAT and click chemistry
PRJEB70937 | ENA
GenomicsTranscriptome-based mapping of m6A sites in newly synthesized mRNA population in U2OS cells.
Transcriptome-based mapping of m6A sites in newly synthesized mRNA population in U2OS cells.
PRJNA902822 | ENA
GenomicsEffects of m6A modification on turnover of newly synthesized mRNAs at the transcriptome level.
Effects of m6A modification on turnover of newly synthesized mRNAs at the transcriptome level.
PRJNA902821 | ENA
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