Sort   by:  
 Page size 
ProteomicsNonionic surfactants can modify the thermal stability of globular and membrane proteins interfering with the thermal proteome profiling principles to identify protein targets
The membrane proteins are essential targets to understand cellular function. The unbiased identification of membrane protein targets is still the bottleneck for a system- level understanding of cellular response to stimuli or perturbations. It has been suggested to enrich the soluble proteome with m...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-03-20 | PXD037153 | Pride
Rat liver tissue Igepal Micelles Nonionic surfactants Thermal proteome profiling Nonidet-p40 substitute 
ProteomicsNatural human tRNAAla anticodon variants mistranslate the genetic code
Transfer RNAs (tRNAs) play an essential role in protein synthesis by linking the nucleic acid sequences of gene products to the amino acid sequences of proteins. Although only 32 tRNAs are needed to decode all 61 sense codons in the genetic code, there are > 400 functional tRNA genes in the humans. ...
ORGANISM(S): Homo sapiens (Human) 
2025-03-07 | PXD053463 | Pride
Anticodon Transfer rna Alanine Mistranslation Missense suppression Protein synthesis 
ProteomicsOncogene-dependent sloppiness in mRNA translation
mRNA translation is a highly conserved and tightly-controlled mechanism for protein synthesis. However, despite protein quality control mechanisms, amino-acid shortage in melanoma induces aberrant proteins by ribosomal frameshifting. The extent and the underlying mechanisms related to this phenomeno...
ORGANISM(S): Homo sapiens (Human) 
2021-09-28 | PXD022707 | Pride
Ribosomal frameshifting Cancer Tryptophan depletion Immunoprecipitation Human Translation Lc-ms/ms 
TranscriptomicsHaematopoietic loss of KDM6A impairs cardiac recovery in heart failure via epigenetic reprogramming of myeloid cells (scATAC-SEQ)
Clonal haematopoiesis (CH), the expansion of blood-cell clones carrying acquired mutations, is an independent risk factor for cardiovascular disease. Although mutations in DNMT3A and TET2 are well studied, the cardiovascular consequences of KDM6A mutations remain unclear. KDM6A is an X-linked histon...
ORGANISM(S): Mus musculus 
2026-09-04 | E-MTAB-17569 | biostudies-arrayexpress
TranscriptomicsSingle-cell ATAC sequencing of SIINFEKL-reactive T cells in GL261 murine brain tumors in MHC class II deficient tumor-mircoenvironments
Single cell ATAC sequencing of SIINFEKL-reactive immune cell from intracranial murine GL261-SIINFEKL tumors 20 days after inoculation. SIINFEKL-reactive T cells were sorted based on dextramer staining. We show that loss of major histocompatibility complex (MHC) class II (MHCII)-restricted antigen pr...
ORGANISM(S): Mus musculus 
2023-01-11 | E-MTAB-12512 | biostudies-arrayexpress
ProteomicsRepurposing Herpesvirus Protein ORF57 to Inhibit TDP-43 Aggregation
The integrated stress response (ISR) is a major element of translation control. The ISR is beneficial in responding to acute stresses, but in human disease a chronic, persistent stress response can contribute to the disease process. Many studies manipulate the ISR by targeting just one protein. Howe...
ORGANISM(S): Homo sapiens (Human) 
2024-08-09 | PXD039246 | Pride
Translational stress response Alzheimer’s disease Als Protein aggregation Tdp-43 
ProteomicsStructural, biochemical and functional characterization of the essential host factor SETD3 in complex with the enterovirus CV-B3 2A protease
Enteroviruses cause a number of medically relevant and widespread human diseases with no approved antiviral therapies currently available. We have previously identified the actin histidine methyltransferase SETD3 as critically important for enterovirus infection through a protein-protein interaction...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD024127 | Pride
Ap-ms Host-pathogen interactions Enterovirus Setd3 2a protease 
ProteomicsA ciliopathy complex restrains centriolar length and builds distal appendages to initiate ciliogenesis.
Mitosis segregates into each daughter cell two centrioles, the older of which is uniquely capable of generating a cilium. How this older centriole, called the mother centriole, initiates ciliogenesis remains poorly understood. We have identified an evolutionarily conserved complex comprised of CEP90...
ORGANISM(S): Homo sapiens (Human) 
2021-06-15 | PXD022372 | Pride
Human Apms Gfp 
ProteomicsStrain- and cell type-specific influenza A virus (IAV)-host protein-protein interaction (PPI) network identified by affinity purification-mass spectrometry (AP-MS)
Influenza A Virus (IAV) is a recurring respiratory virus with antiviral therapies of limited use. Understanding host proteins essential for IAV infection can identify targets for alternative host-directed therapies (HDTs). Using affinity purification-mass spectrometry and global phosphoproteomic and...
ORGANISM(S): Homo sapiens (Human) 
2023-07-25 | PXD036077 | Pride
Virus-host H5n1 Human Ppi H1n1 A549 Nhbe H3n2 Thp-1 Ap-ms Influenza a virus 
TranscriptomicsDisruption of ADNP-KDM1A-GTF2I complex drives neural differentiation imbalance in Helsmoortel-Van der Aa syndrome [multi-omic]
Here, to characterize HVDAS in a developmental setting, we generated cortical brain organoids of 5 control and 5 HVDAS lines and profiled them by single-cell RNA- and ATAC-seq. Organoids were grown for 30 days using the protocol 10.1016/j.stem.2019.08.002. To reduce batch effects and costs, and maxi...
ORGANISM(S): Homo sapiens 
2026-01-27 | E-MTAB-16422 | biostudies-arrayexpress
Sort   by:  
 Page size