Proteins remaining in the metabolomic extract from 3 common extraction modalities were quantified by DIA on the Oribtrap Eclipse following tryptic digest.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090)
SureQuant pTyr, a targeted, tyrosine phosphorylation (pTyr) mass spectrometry method, was applied to profile the pTyr signatures of human colorectal tumor samples. Using internal standard guided acquisition with the SureQuant acquisition mode on the Oribtrap Exploris 480 mass spectrometer (Thermo Sc...
SureQuant pTyr, a targeted, tyrosine phosphorylation (pTyr) mass spectrometry method, was applied to profile the pTyr signatures of human colorectal tumor samples. Using internal standard guided aquisition with the SureQuant acquisition mode on the Oribtrap Exploris 480 mass spectrometer (Thermo Sci...
Candidiasis is the most common invasive fungal infection in clinical sets. The causative agent C. albicans has a complex metabolism for energy production in which mitochondria play important roles for its virulence to the host. Three fungal- specific mitochondrial ETC complex CI subunits - Goa1p, ...
1. Identification of tryptic and chymotryptic fragments in ALECT2 fibrils (Figure 1, Q Exactive HF)
For tryptic: Sample ID 1176342
For Chymotryptic: Sample ID 1176352
2. Intact mass analysis of ALECT2 fibrils from the kidney (Supplementary figure 1 and supplementary table 1, X500B QTOF)
Sample ID:...
Supplemental proteomics data Abstract: Inhibition of ubiquitin E3-ligases is therapeutically attractive; however, the absence of an active-site pocket impedes computational approaches to inhibitor identification. In a large unbiased physical screen, we discovered inhibitors that bind a cryptic cavi...
Covalent chemistry coupled with activity-based protein profiling (ABPP) offers a versatile way to discover ligands for proteins in native biological systems. Here, we describe a set of stereo- and regio-chemically defined spirocycle acrylamides and their analysis in human cancer cells by cysteine-di...
