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ProteomicsFully automated workflow for integrated sample digestion and Evotip loading enabling high-throughput clinical proteomics
Protein identification and quantification is an important tool for biomarker discovery. With the increased sensitivity and speed of modern mass spectrometers, sample-preparation remains a bottleneck for studying large cohorts. To address this issue, we prepared and evaluated a simple and efficient w...
ORGANISM(S): Homo sapiens (Human) 
2024-06-12 | PXD048325 | Pride
Automation Plasma proteomics Dia Biomarkers 
ProteomicsHigh-Throughput Proteomics Sample Preparation Using 96-Channel Pipettor and Magnetic Pin Device
The advent of high-throughput proteomics has created an urgent need for parallel increases in sample processing productivity. However, workflows—particularly those involving PTM profiling—remain highly complex and consist of multiple steps that demand exceptional reproducibility. Many laboratories a...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2026-02-17 | PXD069607 | Pride
Oasis hlb Proteomics Sp3 Phosphoproteomics Pac High-throughput samples preparation 
UnknownLaser Capture Proteomics: spatial tissue molecular profiling from the bench to personalized medicine.
Not available
S-EPMC10720974 | biostudies-literature
ProteomicsAutomated Phosphopeptide Enrichment for Gram Positive Bacteria
Protein phosphorylation in prokaryotes has gained more attention in recent years as several studies linked it to regulatory and signalling functions indicating an importance similar to protein phosphorylation in eukaryotes. Studies on bacterial phosphorylation have so far been conducted using manual...
ORGANISM(S): Listeria monocytogenes EGD 
2021-08-26 | PXD025459 | Pride
Bravo assaymap  fe(iii)-imac  tio2  automation  phosphopeptide enrichment  phosphoproteomics  quantification  listeria monocytogenes  bacillus subtilis  streptococcus pyogenes 
ProteomicsReproducible Automated Phosphopeptide Enrichment using Magnetic TiO2 and Ti-IMAC
We describe the application of magnetic TiO2 and Ti-IMAC for uniform automated phosphopeptide enrichment. Combining hyper-porous magnetic microspheres with a magnetic particle-handling robot enables rapid (45 minutes), reproducible (r2 = 0.80) and high-fidelity (> 90% purity) phosphopeptide purifica...
ORGANISM(S): Mus musculus (Mouse) 
2014-09-22 | PXD000892 | Pride
Phosphoproteomics automation 
ProteomicsAn engineered insulin analog with dual insulin and IGF-1 receptor agonism and distinct signaling
Insulin and insulin-like growth factors (IGF-1 and IGF-2) regulate metabolism, growth, and development via related receptors. In contexts such as brain function or fetal development, coordinated signaling by all three hormones is essential. We report the engineering of [GluB10, D-HisB24, GlyB31, Tyr...
ORGANISM(S): Homo sapiens (Human) 
2026-04-09 | PXD067251 | Pride
Neuroblastoma Sh-sy5y Insulin analog Human Lc-ms/ms 
ProteomicsPhosphorylation ratio determination in fresh frozen and formalin-fixed paraffin embedded tissue with targeted mass spectrometry
Formalin-fixed paraffin embedded (FFPE) tissues are routinely prepared and collected for diagnostics in pathology departments. Therefore, FFPE tissues are the most accessible research sources in pathology archives. In this study we investigated whether we can apply a targeted and quantitative parall...
ORGANISM(S): Homo sapiens (Human) 
2020-08-25 | PXD017943 | Pride
Quantitative proteomics Cancer proteomics Targeted mass spectrometry Phosphorylation ratio Phosphoproteomics Prm Ffpe Clinical proteomics 
ProteomicsThe phosphatases TCPTP, PTPN22, and SHP1 play unique roles in T cell phosphotyrosine maintenance and feedback regulation of the TCR
The protein tyrosine phosphatases (PTPs) TCPTP, PTPN22, and SHP1 are critical regulators of the activating phophotyrosine (pY) site on the initiating T cell kinase, LckY394, but the broader implications of these phophatases in T cell receptor (TCR) signalling and T cell biology remain unclear. By co...
ORGANISM(S): Homo sapiens (Human) 
2025-08-01 | PXD058624 | Pride
Shp1 Phosphoproteomics Tcptp Ptpn22 T cell signaling 
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