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ProteomicsTotal proteome analysis of WT vs TRAP1 KO in HCT-116 and HEK-293T cells grown on different carbon sources.
The molecular chaperone TRAP1, the mitochondrial isoform of cytosolic HSP90, remains poorly understood with respect to its pivotal role in the regulation of mitochondrial metabolism. To characterize the impact of TRAP1 in maintaining the cellular proteome under different energy sources we carried ou...
ORGANISM(S): Homo sapiens (Human) 
2020-01-16 | PXD015828 | Pride
Oxidative phosphorylation Trap1 Mitochondrion Hsp90 Molecular chaperone 
ProteomicsGlobal proteome changes of normal NIH 3T3 versus Ras-transformed NIH cells treated with 3 different Hsp90 inhibitors (geldanamycin, PU-H71 and pochoxime A)
Heat shock protein 90 (Hsp90) is a major hub in the protein network that maintains cellular homeostasis and function. The qualitative and quantitative changes and rewiring of this protein network in tumor cells make them vastly dependent on Hsp90, and as a consequence its inhibition creates a profou...
ORGANISM(S): Mus Musculus (mouse) 
2019-02-11 | PXD009055 | JPOST Repository
TranscriptomicsTranscription profiling of wild type and delta-sba1 yeast treated with radicicol to study gene regulation by sba1
We wanted to study regulation of gene regulation by sba1 (ortholog of mammalian p23) in yeast. sba1/p23 binds to Hsp90 and acts as a co-chaperone. We have a small molecule, radicicol, an analog of geldanamycin to inhibit the binding of p23 to Hsp90. Consequently, we have analyzed the gene expression...
ORGANISM(S): Saccharomyces cerevisiae 
2009-10-14 | E-TABM-573 | biostudies-arrayexpress
TranscriptomicsGenomics of signalling crosstalk of estrogen receptor alpha in breast cancer cells
The estrogen receptor a (ERa) is a ligand-regulated transcription factor. However, a wide variety of other extracellular signals can activate ERa in the absence of estrogen. The impact of these alternate modes of activation on gene expression profiles has not been characterized. We show that estroge...
ORGANISM(S): Homo sapiens 
2008-05-22 | E-GEOD-10466 | biostudies-arrayexpress
ProteomicsTranslational reprogramming in response to accumulating stressors ensures critical threshold levels of Hsp90 for mammalian life
The cytosolic molecular chaperone Hsp90 is essential for eukaryotic life. It is involved in multiple branches of proteostasis and as a molecular capacitor in morphological evolution. Although reduced Hsp90 levels cause phenotypic variations and correlate with aging, whether eukaryotic cells and orga...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-09-20 | PXD031456 | Pride
Hsp90α Stress response Natural selection Development. Hsp90β Stress adaptation Proteostasis Senescence Aging 
ProteomicsModification of the ubiquitinome by Vps11 and Vps18
We quantified by SILAC the modification of the ubiquitinome in human HEK293T cells that overexpressed Vps11 or Vps18
ORGANISM(S): Homo sapiens (Human) 
2019-04-18 | PXD009178 | Pride
Ptmscan Lc-ms Vps11 Vps18 Ubiquitin 
ProteomicsThe Hsp70/hsp90 Co-chaperone Hop/stip1 Shifts the Proteostatic Balance From Folding Towards Degradation
Hop/Stip1/Sti1 is thought to be essential as a co-chaperone to facilitate substrate transfer between the Hsp70 and Hsp90 molecular chaperones. Despite this proposed key function for protein folding and maturation, it is not essential in a number of eukaryotes and bacteria lack an ortholog. We set ou...
ORGANISM(S): Homo sapiens (Human) 
2020-11-09 | PXD012774 | Pride
Molecular chaperone Proteostasis Hop Proteasome Protein folding Hsp70 Protein quality control Hsp90 Protein degradation 
ProteomicsThe mitochondrial HSP90 paralog TRAP1 forms an OXPHOS-regulated tetramer and is involved in maintaining mitochondrial metabolic homeostasis
The molecular chaperone TRAP1, the mitochondrial isoform of cytosolic HSP90, remains poorly understood with respect to its pivotal role in the regulation of mitochondrial metabolism. Most studies have found it to be an inhibitor of mitochondrial oxidative phosphorylation (OXPHOS) and an inducer of t...
ORGANISM(S): Homo sapiens (Human) 
2020-01-16 | PXD015789 | Pride
Oxidative phosphorylation Hsp90 Atp synthase Trap1 Molecular chaperone Mitochondria Glutamine Proteomics 
TranscriptomicsGPR30-mediated estrogen signaling in Estrogen Receptor alpha and beta negative SKBR3 breast cancer cells
Gene expression analyses were carried out to identify genes regulated by 17-beta estradiol (E2) and Hydroxytamoxifen (OHT) through GPR30 in SKBR3 cells, a breast cancer cell-line which expresses GPR30 but lacks Estrogen Receptor alpha or beta. Keywords: Gene expression analysis, Non-genomic signali...
ORGANISM(S): Homo sapiens 
2009-02-19 | E-GEOD-11567 | biostudies-arrayexpress
ProteomicsMolecular chaperone Hsp90 supports adaptive cell size increase in response to chronic stress
Hsf1, well known for its role in acute stress responses, is required for the cell size increase, and that the molecular chaperone Hsp90 is essential for coupling the cell size increase to augmented translation through a translational "rewiring stress response". We propose that this protective proces...
ORGANISM(S): Homo sapiens (Human) 
2023-12-01 | PXD039672 | Pride
Hsf1 Stress adaptation Cytoplasmic density Hsp90 Chronic stress Proteostasis Aging Senescence Cell size Translation 
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