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ProteomicsImproved Sensitivity in Low-Input Proteomics using Micro-Pillar Array-based Chromatography
Capitalizing on the massive increase in sample concentrations which are produced by extremely low elution volumes, nano-LC-ESI-MS/MS is currently the most sensitive analytical technology for the comprehensive characterization of complex protein samples. However, despite tremendous technological impr...
ORGANISM(S): Homo sapiens (Human) 
2019-10-23 | PXD014124 | Pride
Nano-lc-esi-ms/ms nano-flow hplc hela cell digest 
ProteomicsA protocol for robust discovery proteomics using nano liquid chromatography using pillar-array column technology and high-resolution mass spectrometry with data- independent acquisition
The project establishes an easy-to-follow protocol to set up bottom-up proteomics experiments using state-of-the-art pillar-array columns and mass spectrometry. The protocol touches upon key points of the method development, discusses the trade-off between proteome depth and analysis time and demons...
ORGANISM(S): Homo sapiens (Human) 
2026-01-13 | PXD069993 | Pride
Nanolc ms Discovery proteomics Timstof Pillar-array column 
ProteomicsPillar Array Columns for Peptide Separations
Reversed-phase chromatography is the most common technique for separation of tryptic peptides. In this manuscript, we describe the optimization of sample loading and separation parameters for a novel micro machined column and provide a detailed description on the performance and reproducibility of t...
ORGANISM(S): Homo sapiens (Human) 
2019-07-18 | PXD013158 | Pride
Column Pillar Separation 
ProteomicsComparative proteomic profiling of human cell lines using capillary and micro-pillar array column
Chromatographic column selection can impact proteomic profiling, yet comparative studies remain limited. Here, we evaluate the performance of a conventional flame-pulled Accucore resin-packed capillary column and a microfabricated pillar array column (μPAC) in a sample multiplexed global proteome pr...
ORGANISM(S): Homo sapiens (Human) 
2025-11-24 | PXD063964 | Pride
Accucore Chromatography Tmt Μpac Ascend 
ProteomicsEvaluating the Robustness of Micro-Pillar Array Columns for High-Throughput Proteomics
Nanoflow liquid chromatography capable of delivering consistent and reliable results across extensive sample sets is essential for the advancement of mass spectrometry-based proteomics. Micro-Pillar Array Columns (μPACs) represent a significant breakthrough, offering durability and performance stabi...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2025-11-24 | PXD067649 | Pride
Μpac Tandem mass tags Column durability Lc-ms/ms Chromatographic performance 
ProteomicsUltra-sensitive proteomics using second generation micro pillar array LC technology with Orbitrap Exploris™ 480 and FAIMS
In the light of the ongoing single-cell revolution, scientific disciplines are combining forces with the ultimate goal to retrieve as much relevant data as possible from trace amounts of biological material. For single cell proteomics, this implies optimizing the entire workflow from initial cell is...
ORGANISM(S): Homo sapiens (Human) 
2021-06-28 | PXD024017 | Pride
Micro pillar Low-input proteomics Single sell proteomics 
Proteomics15 cm Aurora Gen3 column benchmarking on an Exploris 480
A comprehensive proteome map is essential to elucidate molecular pathways and protein functions. Although great improvements in sample preparation, instrumentation and data analysis already yield-ed impressive results, current studies suffer from a limited proteomic depth and dynamic range there-for...
ORGANISM(S): Homo sapiens (Human) 
2024-02-13 | PXD045457 | Pride
Vanquish neo Orbitrap exploris 480 Trap-and-elute 
ProteomicsBenchmarking of 5.5 cm, 50 cm and 110 cm micropillar array columns as compared to packed bed columns
A comprehensive proteome map is essential to elucidate molecular pathways and protein functions. Although great improvements in sample preparation, instrumentation and data analysis already yield-ed impressive results, current studies suffer from a limited proteomic depth and dynamic range there-for...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2024-02-13 | PXD039576 | Pride
E. coli Yeast Direct injection Micropillar array chromatography Vanquish neo Hela Orbitrap exploris 480 Trap-and-elute 
ProteomicsComparative Proteomic Analysis of Capillary and Micropillar Array Columns Using DIA: Insights from the TKO Yeast Standard
The effective separation of complex peptide mixtures is a cornerstone of mass spectrometry-based proteomics analysis that enhances the accuracy and depth of proteomic analyses. Here we compare datasets collected of whole cell tryptic peptides which were fractionated by either conventional flame-pull...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2025-11-18 | PXD063991 | Pride
Accucore Data-independent acquisition Μpac Tko Ascend 
ProteomicsWide Window Acquisition and AI-based data analysis to reach deep proteome coverage for a wide sample range, including single cell proteomic inputs – Co-immunoprecipitation samples
A comprehensive proteome map is essential to elucidate molecular pathways and protein functions. Although great improvements in sample preparation, instrumentation and data analysis already yielded impressive results, current studies suffer from a limited proteomic depth and dynamic range therefore ...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-13 | PXD037985 | Pride
Mus musculus Co-immunoprecipitation Micropillar array chromatography Smarca5 Orbitrap exploris 480 Ultimate3000 rslcnano Protein-protein interactions Trap-and-elute 
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