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UnknownEnhanced Recombinant Protein Production of Soluble, Highly Active and Immobilizable PNGase F.
Not available
S-EPMC9259526 | biostudies-literature
UnknownSpontaneous and site-specific immobilization of PNGase F via spy chemistry.
Not available
S-EPMC10523939 | biostudies-literature
ProteomicsPNGase-F treated and untreated peptides from primary human mesenchymal stem cells for protein/PTM quant benchmarking
Multivariate regression modelling provides a statistically powerful means of quantifying the effects of a given treatment while compensating for sources of variation and noise, such as variability between human donors and the behaviour of different peptides during mass spectrometry. However, methods...
ORGANISM(S): Homo sapiens (Human) 
2019-03-12 | PXD012782 | Pride
Bayesenproteomics Pngase-f Mesenchymal stem cell 
ProteomicsGlycoproteomes of naive and LPS stimulated human monocytes and THP-1 cells
CD14+ Monocytes from healthy volunteers were purified by MACS (negative selection) and FACSorting and either left untreated or stimulated for 24h and 48h with LPS. THP-1 cells were stimulated for 4h, 24h and 48h with LPS. Glycoproteins were captured with hydrazide chemistry and tryptic and PNGase F-...
ORGANISM(S): Homo sapiens (Human) 
2017-04-24 | PXD004034 | Pride
Monocytes glycoproteomics 
ProteomicsGlycoproteome of CEABAC10 and C57BL6_NRj mice with and without systemic candidiasis
Co-housed young adult CEABAC10-transgenic mice (CEA) with C57BL/6NRj background and their wild type littermates (WT) were injected with 1 × 10*4 CFU C. albicans/g body weight via the lateral tail vein (Ca). Control mice received endotoxin-free buffered saline solution (PBS). Mice were sacrificed aft...
ORGANISM(S): Mus musculus (Mouse) 
2026-05-25 | PXD061893 | Pride
Mouse serum ceabac10 ceacam glycoproteomics 
UnknownAnalysis of PNGase F-Resistant N-Glycopeptides Using SugarQb for Proteome Discoverer 2.1 Reveals Cryptic Substrate Specificities.
Not available
S-EPMC6055662 | biostudies-literature
ProteomicsSepioloidea lineolata MSMS
Sepioloidea lineolata, the striped pyjama squid (family Sepiadariidae), is a small species of benthic squid distributed along the Southern Indo-Pacific coast of Australia. All sepiadariid squids are known to secrete large volumes of viscous slime when stressed. The proteome of S. lineolata slime wa...
ORGANISM(S): Sepioloidea lineolata 
2022-02-28 | PXD010283 | Pride
Glycosylation Squid Comparison Slime Cephalopod 
ProteomicsOVA N-glycans PGC-LC-MSMS - Large-scale identification and visualization of N-glycans with primary structures using GlySeeker
With PNGase F digestion, PGC enrichment,OVA N-glycans were analyzed using PGC nanoLC-ESI-MS/MS.Datasets were analyzed by the N-glycan database search engine called GlycanGoggle developed in our group.
ORGANISM(S): Gallus gallus (Chicken) 
2019-01-17 | PXD006400 | Pride
Pgc-lc-msms Ova N-glycans 
ProteomicsLectin-based affinity enrichment and characterization of N-glycoproteins from the human tear film by mass spectrometry
Glycosylation of proteins is one of the most common post-translational modification (PTM) and plays important regulatory functions in diverse biological processes such as protein stability or cell signaling. Accordingly, glycoproteins are also a consistent part of the human tear film proteome mainta...
ORGANISM(S): Homo sapiens (Human) Sus scrofa domesticus (domestic pig) 
2023-01-25 | PXD038280 | Pride
N-glycoproteins Mass spectrometry Pngase f Tear film Multi-lectin column 
ProteomicsComparative glycomics study of N-glycans in breast cancer cell line MCF7 versus normal cell line
This study designed to characterize the differences in N-glycan expressions between normal and breastcancer cells, MCF10A and MCF7. Permethylated N-glycans released by PNGase F were enriched and then analyzed by LC-ESI/MS. The in-house-developed software GlySeeker was used to search IDs of N-glycan...
ORGANISM(S): Homo sapiens (Human) 
2022-03-02 | PXD010454 | Pride
Lc-esi/ms Breast cancer Mcf7 cell 
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