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ProteomicsPNGaseF-generated N-glycans adduct onto peptides in the gas phase
Glycoproteomics has recently increased in popularity due to instrumental and methodological advances. That said, O-glycoproteomic analysis is still challenging for various reasons, including signal suppression and splitting, search algorithm limitations, and co-occupancy of N- and O-glycopeptides. T...
ORGANISM(S): Homo sapiens (Human) 
2026-06-29 | PXD055856 | Pride
Adducts Glycoproteomics Mucinomics Pngasef Mass spectrometry N-glycans 
ProteomicsOn-tissue spatially-resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscape
Here we present an approach to identify N-linked glycoproteins and deduce their spatial localization using a combination of MALDI N-glycan MSI and spatially-resolved glycoproteomics. We subjected glioma biopsies to on-tissue PNGaseF digestion and MALDI-MSI and found that the glycan HexNAc4-Hex5-NeuA...
ORGANISM(S): Canis lupus 
2023-03-11 | PXD025826 | Pride
Glioblastoma Spatially-resolved Glycoproteomics Haptoglobin Sialic-acid Maldi-msi Hypersialylation Glycan-imaging 
TranscriptomicsRNA-seq analysis of Caenorhabditis elegans exposed to bacterial PNGase enzymes
Peptide:N-glycosidase (PNGase) enzymes produced by bacteria have the potential to influence host cellular physiology. To investigate host transcriptional responses to bacterial PNGase activity, we performed bulk RNA sequencing on whole Caenorhabditis elegans exposed to active PNGaseDJ, PNGaseF, or a...
ORGANISM(S): Caenorhabditis elegans 
2026-01-05 | GSE315009 | GEO
ProteomicsVeneer is a webtool for rapid, standardized, and transparent interpretation, annotation, and reporting of mammalian cell surface glycoprotein capture data
uCSC data of B cells with and without PNGaseF; 4 PNGaseF vendor injections
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-01-19 | MSV000093894 | MassIVE
UnknownNew Enzymatic Approach to Distinguish Fucosylation Isomers of N-Linked Glycans in Tissues Using MALDI Imaging Mass Spectrometry.
Not available
S-EPMC8908332 | biostudies-literature
ProteomicsReceptor Binding, Immune Escape, and Cross-Species Transmission of the Recently Surging SARS-CoV-2 variant BA.3.2.2 (RE.2.2)
Glycoproteomics data of RE.2.2 Spike Protein by nano-LC-MSMS using five protease treatment with PNGaseF in 18O-water and O-glycoprotease
ORGANISM(S): Homo Sapiens Severe Acute Respiratory Syndrome Coronavirus 2 
2026-05-09 | PXD078197 |
UnknownMatrix assisted laser desorption ionization imaging mass spectrometry workflow for spatial profiling analysis of N-linked glycan expression in tissues.
Not available
S-EPMC3969840 | biostudies-literature
ProteomicsGrass carp serum IgM LC-ESI-MS/MS and MALDI-MS
Glycosylation represents a major post-translational modification of proteins that can influence their structure and function.Telesot immunoglobulin M (IgM) is an especially important product of the immune system because it is the main Abs in seum and plays a critical role against defense infection. ...
ORGANISM(S): Ctenopharyngodon idella 
2018-11-29 | PXD010308 | Pride
Lc-ms/ms) Grass carp Immunoglobulin m Maldi-ms 
GenomicsRNA-seq analysis of Caenorhabditis elegans exposed to bacterial PNGase enzymes
RNA-seq analysis of Caenorhabditis elegans exposed to bacterial PNGase enzymes
PRJNA1394779 | ENA
UnknownReal-Time Analysis on Drug-Antibody Ratio of Antibody-Drug Conjugates for Synthesis, Process Optimization, and Quality Control.
Not available
S-EPMC5552727 | biostudies-literature
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