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MetabolomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we d...
2018-07-19 | MTBLS94 | MetaboLights
MetabolomicsIntegrating two chromatographies in a co-fractionation mass spectrometry approach improved protein-metabolite interactions (PMIs) mapping capabilities and revealed novel protein-ligand pairings in Escherichia coli.
The protein-metabolite interactome remains understudied. We previously developed a co-fractionation mass-spectrometry-based approach called PROMIS to address this gap. PROMIS relies on size separation chromatography of protein-metabolite complexes from lysates, followed by proteomics and metabolomic...
2025-04-25 | MTBLS9098 | MetaboLights
ProteomicsDynamic maps of protein-protein-metabolite complexes in S. cerevisiae
The project aims to create dynamic maps of protein-protein-metabolite complexes in S. cerevisiae across growth phases using PROMIS (PROtein–Metabolite Interactions using Size separation). It is a biochemical, untargeted, proteome- and metabolome-wide method to study protein-protein and protein–metab...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-10-25 | PXD026639 | Pride
Protein-metabolite complexes Ligand Protein-protein complexes 
ProteomicsDynamic maps of protein-protein-metabolite complexes of Arabidopsis Thaliana seedlings
The project aimed to create dynamic maps of protein-protein-metabolite complexes of Arabidopsis thaliana seedlings using PROMIS (PROtein–Metabolite Interactions using Size separation). The approach involves using size exclusion chromatography (SEC) to separate complexes, followed by LC-MS-based pro...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-10-14 | PXD026713 | Pride
Protein-metabolite complexes Ligand Protein-protein complexes 
ProteomicsDiscovery of Regulatory Complexes in Arabidopsis via Protein–Metabolite Mapping During Pseudomonas Infection
Protein–metabolite interactions (PMIs) are critical regulators of cellular processes, yet their roles in plant–pathogen interactions remain poorly understood. Here, we applied PROMIS (Protein–Metabolite Interactions using Size separation) to map the Arabidopsis interactome during infection with Pseu...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Pseudomonas syringae pv. tomato str. DC3000 Bacteria 
2026-05-05 | PXD069625 | Pride
Nata1 Promis Protein-metabolite interactions 
ProteomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Herein, we describe a method, dubbed PROMIS that allows simultaneous, global analysis of endogenous protein–small-molecule and protein-protein complexes. To this end a cell-free native lysate was subjected to size-exclusion chromatography followed by quantitative metabolomic and proteomic analysis. ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-06-06 | PXD009714 | Pride
Protein-metabolite Size exclusion chromatografy Deconvolution Correlation Interactions 
UnknownStudies of metabolite-protein interactions: a review.
Not available
S-EPMC4032809 | biostudies-literature
ProteomicsA map of protein-metabolite interactions reveals principles of chemical communication
Application of a novel mass spectrometry-based high-throughput workflow (LiP-SMap) and data resource based on limited proteolysis (LiP) of complex samples. Proteome-wide limited proteolysis sites were obtained in different conditions to infer ligand-induced and protein-protein interaction induced co...
ORGANISM(S): Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2018-01-04 | PXD006543 | Pride
Small molecules Limited proteolysis Lip-smap Dia-ms E.coli 
UnknownPROMIS, global analysis of PROtein-metabolite interactions using size separation in Arabidopsis thaliana.
Not available
S-EPMC6093232 | biostudies-literature
UnknownDifferential radial capillary action of ligand assay for high-throughput detection of protein-metabolite interactions.
Not available
S-EPMC3174574 | biostudies-literature
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