Sort   by:  
 Page size 
ProteomicsSelection of Heating Temperatures Improves the Sensitivity of Proteome Integral Solubility Alteration (PISA) Assay
The thermal shift assay is a robust method of discovering protein-ligand interactions by measuring the alterations in protein thermal stability under various conditions. Several thermal shift assays have been developed and their throughput has been advanced greatly by the rapid progress in tandem ma...
ORGANISM(S): Homo sapiens (Human) 
2020-04-06 | PXD017346 | Pride
Proteome integral solubility alteration (pisa) assay Human cell line lysate Lc-ms/ms 
ProteomicsOne-Pot Time-Induced Proteome Integral Solubility Alteration (OPTI-PISA) assay for automated and sensitive drug target identification
Proteome Integral Solubility Alteration (PISA) assay is widely used for identifying drug targets, but it is labour-, sample and time-consuming. Aiming at enabling automation and greatly reducing the sample amount, we developed One-Pot Time-Induced (OPTI)-PISA. Here we demonstrate OPTI-PISA performan...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD050241 | Pride
Drug target Proteome integral solubility alteration (pisa) One pot Automation 
ProteomicsSystem-wide profiling of drug residence time by proteome integral solubility alteration assay
Most drugs used in the clinic and drug candidates target multiple proteins, and thus detailed characterization of their efficacy targets is required. While current methods rely on quantitative measurements at thermodynamic equilibrium, kinetic parameters such as the residence time of a drug on its t...
ORGANISM(S): Homo sapiens (Human) 
2023-01-06 | PXD034351 | Pride
Target engagement Drug development Thermal proteome profiling. Efficacy target Lead compound optimization Target prioritization Binding affinity Precision medicine Target occupancy 
ProteomicsProteome integral solubility alteration assay combined with multi-criteria decision-making analysis for developing adverse outcome pathways
To simplify chemical risk assessment, the adverse outcome pathway (AOP) method has arisen as a framework to predict the impact of chemical exposure on human and environmental health. The development of this predictive tool requires knowledge of the molecular interaction between chemicals and protein...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD033056 | Pride
Hepatocytes Bioactive compound Pollutants Proteome integral solubility alteration assay Tcdd Analytical hierarchy process Hepg2 
ProteomicsStreamlined analysis of drug targets by proteome integral solubility alteration indicates organ-specific engagement
Proteins are the primary targets of almost all small molecule drugs. However, even the most selectively designed drugs can potentially target several unknown proteins. Identification of potential drug targets can facilitate design of new drugs and repurposing of existing ones. Current state-of-the-a...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2024-10-16 | PXD050784 | Pride
Drug target Small molecule Target identification Chemical proteomics Tpp Pisa 
ProteomicsProteome Integral Solubility Alteration (PISA): a high-throughput proteomics assay for target deconvolution
Various agents, including drugs as well as non-molecular influences, induce alterations in the physic-chemical properties of proteins in cell lysates, living cells and organisms. These alterations can be probed by applying a stability-modifying factor, such as elevated temperature, to a varying degr...
ORGANISM(S): Homo sapiens (Human) 
2020-04-01 | PXD013182 | Pride
Protein stability Target deconvolution Tmt Thermal profiling Quantitative proteomics 
UnknownSelection of Heating Temperatures Improves the Sensitivity of the Proteome Integral Solubility Alteration Assay.
Not available
S-EPMC7294736 | biostudies-literature
ProteomicsProteome Integral Solubility Alteration assay in zebrafish embryo for exploring exposure to bioactive compounds including pollutants and chemicals mixtures
The One Health initiative connects human and animal health with their shared environment. The development of novel methodology to unbiased identification of mechanisms of action of chemicals and chemicals mixtures would address one of today´s challenges of environmental and human toxicology. Zebrafi...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2022-02-28 | PXD021670 | Pride
Thermal proteome profiling Bioactive compound Pollutants Drug target Zebrafish embryo Biodiscovery Novel drug Proteome integral solubility alteration assay Chemical mixtures 
ProteomicsProteome integral solubility alteration assay for monitoring antibody-antigen interactions in cells
Proteome Integral Solubility Alteration (PISA) assay is normally used for characterization of the interactions between a small molecule and the cellular proteome. Here we modified PISA uniquely to identify the antigen (tumor necrosis factor alpha) in activated monocytes using the monoclonal antibody...
ORGANISM(S): Homo sapiens (Human) 
2026-05-20 | PXD065457 | Pride
Pisa Antibody Antigen 
ProteomicsProteome integral solubility alteration high-throughput proteomics assay identifies Collectin-12 as a non-apoptotic microglial caspase-3 substrate.
Caspases are a family of proteins mostly known for their role in the activation of the apoptotic pathway leading to cell death. In the last decade, caspases have been found to fulfil other tasks regulating the cell phenotype independently to cell death. Microglia are the immune cells of the brain re...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-10 | PXD032835 | Pride
Caspase-3 Solubility Pisa Non-apoptotic Sustrates Colec12 Proteomics Microglia 
Sort   by:  
 Page size