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ProteomicsPulse-chase SILAC of Mycobacterium tuberculosis infected THP-1 macrophages
Pulse chase SILAC was used to identify protein turnover within human macrophages infected with mycobacterium tuberculosis CDC1551, a ppe38-71 mutant strain, a complemented strain and an uninfected control.
ORGANISM(S): Homo sapiens (Human) 
2021-06-15 | PXD021166 | Pride
Lc-ms/ms Tuberculosis Silac Macrophages 
ProteomicsA novel pulse-chase SILAC strategy measures changes in protein decay and synthesis rates induced by perturbation of proteostasis with an Hsp90 inhibitor.
We developed a pulse-chase variant of SILAC (stable isotope labeling by amino acids in cell culture pcSILAC). pcSILAC can quantitate in one experiment and for two conditions the relative levels of proteins newly synthesized in a given time as well as the relative levels of remaining preexisting prot...
ORGANISM(S): Homo sapiens (Human) 
2013-12-10 | PXD000538 | Pride
Hsp90 T-cells Pulse-chase Silac Synthesis rate Decay rate 
ProteomicsPulse-chase SILAC analysis of mitochondrial complex assembly
Pulse (P) - Chase (C) SILAC analysis of mitochondrial complex assembly Bogenhagen et al (2018) Cell Reports Pulse-chase times in hours (P#C#)
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-01-03 | MSV000084750 | MassIVE
Proteomics3T3-L1 adipocyte protein turnover
Insulin is a potent regulator of protein metabolism. Here we describe a time-resolved map of insulin-regulated protein turnover in 3T3-L1 adipocytes using metabolic pulse-chase labelling and high-resolution mass spectrometry.
ORGANISM(S): Mus musculus (Mouse) 
2016-10-07 | PXD003696 | Pride
Proteostasis Pulse-chase silac Protein synthesis Insulin 
UnknownPulse-chase SILAC-based analyses reveal selective oversynthesis and rapid turnover of mitochondrial protein components of respiratory complexes.
Not available
S-EPMC7049976 | biostudies-literature
ProteomicsProtein turnover dynamics of IAV-infected HeLa cells
The abundance of a protein is defined by its continuous synthesis and degradation, a process known as protein turnover. Here, we systematically profiled the turnover of proteins in influenza A virus (IAV) infected cells using a pulse-chase SILAC-based approach combined with downstream statistical mo...
ORGANISM(S): Homo sapiens (Human) 
2025-04-09 | PXD047063 | Pride
Iav Pulse-chase Silac Hela Protein turnover Lc-msms 
ProteomicsNIH 3T3 AHA p-c, SILAC p-c, inhibitor, and enrichment control experiments
We developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. These experiments were performed with 0, 1, 2, 4, 8, 16 and 13 h of chase. Data from 3 biological replicates ar...
ORGANISM(S): Mus musculus (Mouse) 
2016-10-06 | PXD004929 | Pride
Azidohomoalanine Mouse Silac Degradation Aneuploidy 
UnknownA novel pulse-chase SILAC strategy measures changes in protein decay and synthesis rates induced by perturbation of proteostasis with an Hsp90 inhibitor.
Not available
S-EPMC3842330 | biostudies-literature
ProteomicsImproving SILAC quantification with data independent acquisition to investigate bortezomib-induced protein degradation
Stable isotope labeling by amino acids in cell culture (SILAC) coupled to data-dependent acquisition (DDA) is a common approach to quantitative proteomics with the desirable benefit of reducing batch effects during sample processing and data acquisition. More recently, using data-independent acquisi...
ORGANISM(S): Homo Sapiens 
2022-03-29 | PXD022659 | panorama
UnknownSpatial Stable Isotopic Labeling by Amino Acids in Cell Culture: Pulse-Chase Labeling of Three-Dimensional Multicellular Spheroids for Global Proteome Analysis.
Not available
S-EPMC9248019 | biostudies-literature
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