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ProteomicsConfirmation of direct interaction between H3K56cr and GLYR1 by H3K*56cr Site-Link in living cells
As we have found that H3K56cr can crosslink with GLYR1 in living cells, we now perform H3K*cr Site-Link in GLYR1 overexpressed cells. The subsequent 365-nm light irradiation could activate the diazirine and covalently capture potential effector proteins on native chromatin in living cells. The cross...
ORGANISM(S): Homo sapiens (Human) 
2023-03-06 | PXD033960 | Pride
Plink Glyr1 H3k56cr 
ProteomicsSite-Link for identification of chromatinized proteome defined by site-specific histone acylations in living cells
By coupling the mass spectrometry, we developed the Site-Link strategy based on the GCE method for analyzing the interacting proteome of histone lysine acylations in living cells. As K* could mimic the natural lysine to be recognized by natural lysine synthase, histones bearing the site-specifically...
ORGANISM(S): Homo sapiens (Human) 
2023-03-06 | PXD033959 | Pride
K*56pabk* Site-link K*37cr K*56ac 
ProteomicsLife-time study of premodified histone for Identification of chromatinized proteome defined by site-specific histone acylations in living cells
C13, N15 isotope labelled heavy lysine bearing the modification group, such as cr and bhb, were site-sepcifically incorporated into histone H3K56 in living cells for measurement of the modification life of H3K56cr and H3K56bhb without iinterference caused by endogenous histone modification. Utilizai...
ORGANISM(S): Homo sapiens (Human) 
2023-03-06 | PXD033958 | Pride
Heavy lysine H3k56 Life time Bhb Cr 
ProteomicsIdentification and profiling of dynamic changes of site-specific histone acylations in HEK293T cells under metabolites treatment
1. Systematic survey of PTM site and stoichiometry on histone proteins has been lagged behind due to the lack of efficient quantitative peptide comparison methodology on histone proteins. Our quantitative mass spectrometry-based proteomics approach, Site-Profiling, used SILAC-based peptide ratio ana...
ORGANISM(S): Homo sapiens (Human) 
2023-03-06 | PXD029508 | Pride
Hek293t Histone acylation Site-specific Lc-ms/ms 
ProteomicsSite-Link for identification of chromatinized proteome defined by site-specific histone acylations in living cells
By coupling the mass spectrometry, we developed the Site-Link strategy based on the GCE method for analyzing the interacting proteome of histone lysine acylations in living cells. As K* could mimic the natural lysine to be recognized by natural lysine synthase, histones bearing the site-specifical...
ORGANISM(S): Homo sapiens (Human) 
2023-03-06 | PXD029505 | Pride
Hek293t Histone acylation Site-specific Proteome Lc-ms/ms 
ProteomicsIdentification of site-specific incorporation of Kacyl/K*acyl in full-length histones in E. coli and mammalian cells
With the evolution of the PylRS system, we identified the PylRS variants for incorporation of all synthesized Kacyl and Kacyl* in the full-length H3 protein both in E. coli and mammalian cells, which covered the tail domain (eg. K23, K27, K56) as well as the globular domain that are difficult for ch...
ORGANISM(S): Homo sapiens (Human) 
2023-03-06 | PXD029504 | Pride
Purified proteins Hek293t E. coli Lc-ms/ms 
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